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首页> 外文期刊>Journal of Biotechnology >Applicability of Euglena gracilis for biorefineries demonstrated by the production of alpha-tocopherol and paramylon followed by anaerobic digestion
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Applicability of Euglena gracilis for biorefineries demonstrated by the production of alpha-tocopherol and paramylon followed by anaerobic digestion

机译：通过生产α-生育酚和副甲酰胺，然后进行厌氧消化证明了Euglena gracilis在生物精炼厂中的适用性

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In this study the use of Euglena gracilis biomass for ce.-tocopherol, paramylon and biogas production in a value-added chain was investigated. Therefore, we analyzed the dry cell weight and product concentrations at different growth phases during heterotrophic, photoheterotrophic and photoautotrophic cultivation in a low-cost minimal medium. Furthermore, the specific biogas yields for differently derived biomass with and without product recovery were investigated. We demonstrate that growth phase and cultivation mode not only have a significant impact on product formation, but also influence the yield of biogas obtained from anaerobic digestion of Euglena gracilis biomass. The maximum dry cell weight concentration ranged from 12.3 +/- 0.14 g L-1 for heterotrophically to 3.4 +/- 0.02 g L-1 for photoautotrophically grown Euglena gracilis cells. The heterotrophically grown biomass accumulated product concentrations of 5.3 +/- 0.12 mg L-1 of alpha-tocopherol and 9.3 +/- 0.1 g L-1 of paramylon or 805 +/- 10.9 mL of biogas g(-1) (per gram volatile solids). The results for photoautotrophically grown cells were 8.6 +/- 0.22 mg L-1 of alpha-tocopherol and 0.78 +/- 0.01 g L-1 of paramylon or 648 +/- 7.2 mL of biogas g(-1). For an energy-saving downstream procedure the extracting agent methanol does not have to be removed strictly. Samples with residual methanol showed a significantly increased biogas yield, because the solvent can be used as an additional substrate for methane production by archaebacteria. (C) 2015 Elsevier B.V. All rights reserved.
机译：在这项研究中，研究了在一条增值链中使用Euglena gracilis生物质生产ce-生育酚，副mylon和沼气的方法。因此，我们分析了在低成本基本培养基中异养，光异养和光自养培养期间不同生长阶段的干细胞重量和产物浓度。此外，研究了在有或没有产品回收的情况下，不同来源的生物质的特定沼气产量。我们证明，生长期和耕种方式不仅对产品形成有重要影响，而且还影响了从裸藻的生物质厌氧消化获得的沼气产量。干细胞的最大干重浓度范围从异养的12.3 +/- 0.14 g L-1到光养自养的Euglena gracilis细胞的3.4 +/- 0.02 g L-1。异养生长的生物质累积的产物浓度为5.3 +/- 0.12 mg L-1的α-生育酚和9.3 +/- 0.1 g L-1的对mylon或805 +/- 10.9 mL的沼气g（-1）（每克挥发性固体）。光合自养细胞的结果是8.6 +/- 0.22 mg L-1的α-生育酚和0.78 +/- 0.01 g L-1的对mymylon或648 +/- 7.2 mL沼气g（-1）。对于下游的节能程序，不必严格去除萃取剂甲醇。残留甲醇的样品显示沼气产率显着提高，因为该溶剂可以用作古细菌生产甲烷的另一种底物。 （C）2015 Elsevier B.V.保留所有权利。

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