The sterile seedlings of Hibiscus esculentus L.were obtained via sterile germination.The stem segments with one leaf and one axillary bud could be repeatedly subcultured on the medium"MS+1 0?mg/L IBA", and a propagation coefficient is 3-4 in each subculture. KT had a strong inhibiting effect on the germination and growth of the axillary buds of Hibiscus esculentus L., and a strong dedifferentiating effect on the stem segments. About 25% of the calli, cultured on the medium"MS+0 5-1 0?mg/L IBA" for 50-60 days,transformed into somatic embryoids, which were visible to the naked eye. Most of the somatic embryoids will develope into the intact plantlets on the same medium 30 days later.
展开▼
机译：石斛兰"绿色星辰×出水芙蓉"杂交种子无菌萌发与组培快繁研究Sterile Germination of the Hybrid Seeds of Den. Burana Green Star × Den. Rainbow-compactum and Rapid Propagation of F1 Plantlets via Tissue Culture
