摘要：
Objective To investigate the effects of penehyclidine hydrochloride injection combined with safflower injection on TLRs/NF-κB signaling pathway and T cell subsets in mononuclear cells of patients with organophosphorus pesticide poisoning. Methods 80 cases of organophosphate poisoning patients from June 2014 to June 2016 in onglu first people's hospital were selected,randomly divided into control group and observation group, the control group was treated with penehyclidine hydrochloride,observation group were treated with Honghua injection based on the treatment of control group. The effects were compared between two groups of TLRs/NF-κB in peripheral blood mononuclear cells pathway and T cell subsets in peripheral blood, and compared two groups of patients with cholinesterase activity. Results On the third,fifth and 7th day of treatment,cholinesterase activity in both groups was higher than that before treatment,the activity of cholinesterase in the observation group was significantly higher than that in the control group (P<0.05).After treatment,compared with before treatment,the levels of TLR2,TLR4 and NF-κB in the two groups were significantly decreased,and TLR2,TLR4,NF-κB levels in the observation group were lower than those in the control group, the difference was statistically significant (P<0.05),Th17,Th17/Treg levels in the observation group were significantly lower,and lower than the control group,Treg content was significantly higher,and higher than the control group,the difference was statistically significant (P<0.05). Conclusion Penehyclidine hydrochloride injection combined with safflower injection can help to reduce Th17,Th17/Treg levels,increased Treg cell content,regulate the inflammatory response, and promote recovery of cholinesterase activity.%目的 探讨盐酸戊乙奎醚注射液联合红花注射液对有机磷中毒患者外周血单个核细胞核外周血T细胞亚群的影响.方法 选取上海瑞金医院桐庐分院第一人民医院2014年6月~2016年6月收治的80例有机磷中毒患者,随机分为对照组与观察组,对照组患者采用盐酸戊乙奎醚注射液治疗,观察组患者在对照组基础上联合红花注射液,对比2组患者外周血单个核细胞TLRs/NF-κB信号通路及外周血T细胞亚群水平,比较2组患者胆碱酯酶活性.结果 治疗第3、5、7天,2组患者胆碱酯酶活性与治疗前比较均升高,且观察组各时间点胆碱酯酶活性均明显高于对照组(P<0.05).治疗后,与治疗前比较,2组患者TLR2、TLR4、NF-κB水平均显著降低,且观察组TLR2、TLR4、NF-κB水平低于对照组,差异均具有统计学意义(P<0.05);观察组Th17、Th17/Treg细胞均明显降低,且低于对照组,Treg含量明显升高,且高于对照组,差异具有统计学意义(P<0.05).结论 盐酸戊乙奎醚注射液联合红花注射液有助于降低Th17、Th17/Treg水平,提高Treg细胞含量,调节炎症反应,促进胆碱酯酶活性恢复.
