• 主题
高级搜索  >
历史搜索 清空历史
您现在的位置：首页> 中文期刊>基础医学>医药卫生>现代生物医学进展

现代生物医学进展

现代生物医学进展

CSTPCD

Progress in Modern Biomedicine
《现代生物医学进展》是国家科技部中国科技论文统计源期刊，中国科技核心期刊。国内统一刊号： CN 23-1544/R 国际标准刊号：ISSN 1671-2285 月刊 邮发代号：14-12 定价：9元/期 本刊网址: http://swcx.periodicals.net.cn http://swcx.chinajournal.net.cn .本刊原刊名为《生物磁学》,（详见科技部信息所网站：http://cstpcd.istic.ac.cn），据科技部信息所2005年版的中国科技期刊引证报告，本刊影响因子0.734，在本学科（生物学）排名列第9位，在1608种统计源核心期刊总排名列第169位.刊名变更是本刊的自然过渡,已经国家新闻出版总署新出报刊[2006]4号批准。本刊原刊名为《生物磁学》,（详见科技部信息所网站：http://cstpcd.istic.ac.cn），据科技部信息所2005年版的中国科技期刊引证报告，本刊影响因子0.734，在本学科（生物学）排名列第9位，在1608种统计源核心期刊总排名列第169位.刊名变更是本刊的自然过渡,已经国家新闻出版总署新出报刊[2006]4号批准。本刊已经经国务院新闻办、国家新闻出版总署审核备案，已被科技部中国科技论文与引文数据库(CSTPCD)、中国科技文献数据库(CSTDB)、中国期刊全文数据库(CJFD)、中国学术期刊综合评价数据库、《中国期刊网》、《中国学术期刊》(光盘版)、科技部中文科技期刊数据库，《中国生物学文摘》,中国生物学文献数据库,中国生物医学文献数据库(CBM disc)、中文生物医学期刊文献数据库(CMCC)等权威数据库收录。
更多
  • 发文量：25739
  • 被引量：13390
  • H指数：1
  • 开始收录时间：1999(1)
  • CNKI综合因子：0.803
  • 维普期刊影响因子： 1.59
  • 万方期刊影响因子：1.7
  • 创刊时间：2001
  • 国内刊号/CN：23-1544/R
    国际刊号/ISSN：1673-6273
  • 发行周期：旬刊
    邮发代号：14-12
  • 主管单位：黑龙江省卫生厅
    主办单位：黑龙江省森工总医院 哈尔滨医科大学附属第四医院
现代生物医学进展-联系信息
  • 主编：徐万海
  • 电话：0451-82583800 53658268
  • 邮箱：biomed_54@126.com;liudhui_21@126.com；biomagnetism@
  • 地址：哈尔滨市54号信箱（(哈尔滨市花园街184号403）
  • 邮编：150001
现代生物医学进展-获奖情况
  • 暂无获奖信息
  • 收录汇总
  • 刊内文献检索
  • 投稿信息

刊期浏览

相关中文期刊

相关中文会议

相关外文期刊

相关外文会议

最新期刊

热门期刊

现代生物医学进展 >2023年第23期
搜索

学科

年度

全选（0）
排序：
共4384条结果
  • 摘要：Objective:To analyze the allergic ocular surface disease clinical features and relevant treatment methods (To analyze clinical features and the relevant treatment methods of allergic ocular surface diseases).Methods:100 patients with allergic ocular surface diseases,were retrospectively analyzed,by analyzing the clinical features of allergic ocular surface disease,includingmedical history,symptoms,signs and the clinical data of diagnosis and treatment of the corresponding.Results:50 patients with allergic ocular history,90 patients showed eye itch,89 patients showed foreign body sensation,88 patients showed conjunctival congestion,76 patients with upper eyelid conjunctival papilla,70 patients with follicular rate upper tarsal conjunctiva.45 patients with perennial allergic conjunctivitis,30 patients with specific conjunctivitis,10 patients with giant papillary conjunctivitis,80 patients with allergic conjunctivitis,the positive rate of eosinophils into 100％.After treatment,57 patients were alleviated,7 cases were improved,30 cases of patients with the disease under control,and 9 cases of critically ill patients in the acute stage of application of hormone treatment,the clinical symptom of 6 cases had no obvious improvement.Conclusion:The clinical symptoms were allergic ocular surface disease greatly,make accurate judgment on the clinical features of allergic eye disease patients and treatment is correct,there is certain clinical significance in restoration of allergic eye disease patients.%目的:对过敏性眼表疾病的临床特点和相关的诊治方法进行分析探讨.方法:选取100例过敏性眼表疾病的患者,对其相应的临床资料进行回顾性分析、通过对过敏性眼表疾病的病史、症状、体征等临床特点进行统计,进而对诊治方法进行分析探讨.结果:50例患者有过敏性眼病史,90例患者表现为眼痒,89例患者表现有异物感,88例患者表现为结膜充血,76例患者表现有上眼睑结膜乳头,70例患者表现有上眼睑结膜滤泡.45例患者为常年过敏性结膜炎,30例患者为特异性结膜炎,10例患者为巨乳头结膜炎,80例患者为过敏性结膜炎,嗜酸性粒细胞的阳性率为100％.经过治疗后,57例患者得到缓解,7例患者得到明显改善,30例患者病情得到控制,且有9例重症患者在急性期应用了激素治疗,6例患者临床症状无明显改善.结论:过敏性眼表疾病的临床症状表现差异较大,对过敏性眼表疾病患者的临床特点做出准确的判断并给出正确及时的治疗,对过敏性眼表疾病患者的恢复有一定的临床意义.
  • 摘要：目的:探讨彩色高频超声多普勒在男性乳腺增生症中的应用价值.方法:收集我院2010年8月至2012年9月门诊及住院疑似男性乳腺增生症患者63例,应用彩色高频超声对63例(77只)男性乳腺增生症患者检查,分析其声像图特点并采用自身前后对照的方法与临床走访或病理诊断相比较.结果:超声诊断男性乳腺增生60例(74只),疑为男性乳腺癌2例,疑为增生伴炎性改变1例.临床或病理证实男性乳腺增生63例(77只).彩色高频超声多普勒在诊断男性乳腺增生上与临床金标准相比,符合率为96.1％.结论:彩色高频超声诊断男性乳腺增生症是一种准确、无创、简便的方法,有较高的应用价值.%Objective:To discuss the application value of High frequency color Doppler ultrasound in gynecomastia.Methods:63 males (77 breasts) with gynecomastia were examined using high frequency color Doppler ultrasound,analyzed the imaging features and compared with clinical gold standard diagnosis.Results:60 cases (74 breasts) gynecomastia were diagnosed by Ultrasound,2 cases were suspected breast cancer,1 cases was suspected inflammatory changes.63 cases(77 breasts) were diagnosed by clinical or pathologic.High frequency color doppler ultrasound in the diagnosis of male on hyperplasia of mammary glands compared with clinical gold standard,the coincidence rate was 96.1％.Conclusion:The diagnosis of High frequency color Doppler ultrasound for gynecomastia is a accurate,non-invasive and simple method and has higher application value.
  • 摘要：目的:肺动脉高压患者的预后非常差并且目前对肺动脉高压的治疗缺乏有效的方法.因此,对肺动脉高压的研究十分迫切,而建立稳定、操作简便、稳定的肺动脉高压模型是研究肺动脉高压的基础.本实验的目的就是采用不同剂量野百合碱(monocrotaline,MCT)诱导建立SD大鼠肺动脉高压模型,探讨肺动脉高压模型的制作方法及其稳定性.方法:清洁级SD雄性大鼠75只,随机分为C组、M1、M2、M3和M4组,每组各15只.其中M1～M4组大鼠分别一次性腹腔注射MCT 30、40、50和60 mg/kg,诱导制作肺动脉高压动物模型;C组为对照组,给予相同剂量溶剂腹腔注射.腹腔注射4周后比较各组大鼠的生存率、检测平均肺动脉压力(mPAP),肺动脉收缩压(PASP),右心肥大指数(RVHI),并取肺组织行苏木素-伊红染色,观察肺的病理改变,采用肺小动脉形态计量学指标综合判断肺动脉高压模型的稳定性.结果:C组无死亡,M1～M4组大鼠的生存率分别为87％,87％,67％,40％.M1～M4各组SD大鼠平均肺动脉压、肺动脉收缩压、右心肥大指数依次增大(P＜0.05),肺小动脉形态计量学指标检测显示肺血管中膜厚度百分比依次增大(P＜0.05),而M3与M4组各测量结果无明显差异.结论:腹腔注射50 mg/kg、60 mg/kg剂量野百合碱均可引起大鼠肺动脉压力升高和肺血管重构,均可诱导稳定的肺动脉高压模型,而50 mg/kg剂量有更高的生存率.所以50mg/kg剂量野百合碱腹腔注射是成功诱导大鼠肺动脉高压模型的合适剂量.%Objective:The prognosis of patients with pulmonary arterial hypertension (PAH) is poor,and at present there is no effective treatment for PAH.Therefore,the study for PAH is very urgent,and to build stable,user-friendly controlled and applicative pulmonary arterial hypertension animal model is the basis for researching pulmonary arterial hypertension.The objective of our experiment is to establish PAH model by using different doses of monocrotaline (MC.T) in SD rats and to explore the optimal method for model establishment.Methods:Seventy-five SPF Sprague-Dawley male rats were randomly divided into 5 groups,and there was 15 rats in each group.Different doses of monocrotaline of 30,40,50 and 60 mg / kg were given to rats in Group M1 to M4 through intraperitoneal injection respectively in order to establish the model of pulmonary arterial hypertension; and rats in Group C (control group) were given the same solvent.The survival rate,mean pulmonary arterial pressure(mPAP),pulmonary arterial systolic pressure(PASP),right ventricle hypertrophy index(RVHI),the microstructural alteration of small pulmonary vessel were measured and tissue sections of lung were made after intraperitoneal injection for 4 weeks.Results:The rats in group C were all alive.The survival rate of rats in M1 ～M4 were 87％,87％,67％ and 40％,respectively.The mean pulmonary arterial pressure,pulmonary arterial systolic pressure and right ventricle hypertrophy index in M1 ～M4 increased gradually (P＜0.05),the morphometry indices of small pulmonary arteries reveal relative medial thickness increased gradually (P＜0.05),but there is no significant difference in M3 and M4.Conclusion:Dose of MCT of 50 mg/kg and 60mg/kg will cause to rise pulmonary arterial pressure and remodel vascular of rats,both of which can induce the stable pulmonary arterial pressure model,while dose of 50mg/kg has higher survival rate.So,intraperitioneal injection of monocrotaline with dose of 50 mg/kg is the appropriate dose to induce rats pulmonary arterial pressure model.
  • 摘要：目的:观察氧化苦参碱(oxymatrine,OMT)对骨髓来源细胞增殖的影响.方法:应用MTr比色法、流式细胞仪检测法、集落形成法和免疫细胞活性测定等方法,检测OMT对白血病细胞K562的抑制作用;骨髓造血干细胞的集落形成实验和脾细胞对肿瘤细胞的杀伤的生物活性试验,检测OMT对小鼠免疫和造血的影响.结果:(1)不同浓度的OMT可明显抑制白血病细胞K562细胞的增殖、集落形成,导致细胞凋亡(P＜0.05),且作用呈浓度依赖性.(2)OMT可以促进小鼠骨髓粒系造血,且在0.2475 mg/mL时达到峰值.(3)OMT可抑制小鼠的免疫功能.结论:OMT可抑制白血病K562细胞的增殖并诱导其凋亡,同时抑制小鼠的免疫功能,促进小鼠骨髓CFU-GM的形成,表现出对骨髓来源细胞生长的双向调节作用.%Objective:To study the effect of Oxymatrine on the proliferation of cells derived from bone marrow.Methods:MTT assay,flow cytometry analysis,cell colony formation assay and cell immune activity determination method were used to study the effect of Oxymatrine on Chronic myeloid leukemia cell line K562.Cell colony formation assay of Bone marrow hematopoietic stem cells and killing effect of spleen cells on tumor cells were used to detect the influence of Oxymatrine on immune and hematopoietic system of mouse.Results:Oxymatrine significantly inhibited the proliferation of K562 cells,induced the apoptosis and inhibited the formation of cell colony in a concentration dependent manner.Meanwhile,it also suppressed the immune function and promote the hematopoietic function of mouse.Conclusion:Oxymatrine could inhibit the proliferation and induce the apoptosis in K562 cells,meanwhile suppress the immune function of mouse and promote the formation of CFU-GM of bone marrow of mouse,which showed two-way regulation on cells derived from bone marrow.
  • 摘要：目的:考察非小细胞肺癌患者化疗后对HHPG-19K的耐受性及该药物的安全性.方法:随机将招募受试者30例平均分成设五个试验组:HHPG-19K 3个剂量组(60 μg/Kg、100 μg/Kg、200 μg/Kg)、阳性对照组(惠尔血,即G-CSF 5 μg/Kg/天)和阴性对照组,对比5组的安全性观察指标.结果:3个剂量组均出现不良事件,占总人数的100％;另外,实验室检查值的异常主要有ALP、ALT及AST升高等.不良事件的类型和严重程度均为轻中度,与阳性对照组无差异(P＜0.05).而阴性对照组发生不良事件为13％,少于剂量组与阳性对照组(P＜0.05).结论:试验用药物HHPG-19K在非小细胞肺癌化疗患者中具有较好的耐受性,且不产生耐药性抗体,其中100 μg/kg安全性更好.%Objective:To investigate the tolerance of the patients with non-small cell lung cancer after chemotherapy for HHPG-19 k and the drug safety.Methods:Randomly recruit 30 cases and divide into five treatment group in average:HHPG-19k three dose group (60 μg/Kg,100 μg/Kg,200 μg/Kg),positive control group (nuier blood,namely g-CSF5 μg/Kg/day) and negative control group,compared to 5 groups of safety observation index.Results:Three dose groups all appear adverse events,accounting for 100 ％ of the total number of; In addition,laboratory examination of abnormal value mainly ALP,ALT and AST rise,etc.Adverse events type and severity both are mild and moderate,and has no difference with the positive control group (P＜0.05).And the proportion of adverse events in the negative control group is 13％,less than the dose group and the positive control group (P＜0.05).Conclusion:Test the use of drugs HHPG -19 k in non-small cell lung cancer patients with chemotherapy has a good tolerance,and do not produce resistance antibody,of which 100 μ g/kg better safety.
  • 摘要：Objective:To investigate the optimum conditions for the extraction of flavonoids from turmeric by using methodology.Methods:On the basis of one-factor-at-a-time experiments,Box-Benhnken central composite design was applied to investigate the effects of ultrasonic power,ultrasonic time,liquid-to-solid ratio and ethanol concentration on extraction yield of flavonoids.Results:According to Box-Benhnken principle of design,the response surface method included four response factors and three levels determined good conditions,which were ultrasonic power 400.76 w,ultrasonic time 29.53 min,liquid-to-solid ratio 1:42.19 and ethanol concentration 79.60 ％.In these conditions,the extraction yield of flavonoids was 11.15 (mg/g).Conclusion:Considering the practical problems,the best extraction technology parameter for ultrasonic power 400 w,ultrasonic time 30 min,liquid-to-solid ratio 1:40 and ethanol concentration 80 ％ were confirmed.Three experiments were operated,results showed that flavonoids extraction ratio was 11.09 (mg/g),and this condition was very close to the predicted value,absolute error was 0.057 ％,so the experimental results and the model has a good agreement.%目的:利用响应面法对黄姜总黄酮的提取工艺进行优化.方法:在单因素的基础上以超声功率、超声时间、料液比、乙醇浓度为响应因素,黄姜总黄酮的提取率为响应值,根据中心组合实验原理,采用四因素三水平的响应面分析法.结果:确定提取最佳工艺参数为超声时间29.53 min、超声功率为400.76w、料液比为1:42.19、乙醇浓度为79.60％,在此条件下总黄酮提取率的预测值为11.15 (mg/g).结论:考虑到实际操作的问题,将提取最佳工艺参数修正为超声时间30 min、超声功率400w、料液比1:40、乙醇浓度80％,进行3次平行试验,结果总黄酮提取率为11.09 mg/g,绝对误差为0.057％,说明实验结果与模型吻合很好.
  • 摘要：Objective:To observe the impact of plating position of the reconstruction plate on complicated clavicle midshaft fracture.Methods:Eighty-eight patients with complicated clavicle midshaR fracture that was classified as type C,according to the AO/OTA classification,who were treated by open reduction and internal fixation with a reconstruction plate.Thirty-seven patients were treated with the reconstruction plate in an anterior position,while the rest fifty-one patients were in a superior position.The mean time to union,incidence of complications (nonunion,delayed union,hardware failure,hypertrophic scarring) and Constant Murley Score were observed and analyzed to make comparisons between the two groups.Results:The mean time to union was 11.8 weeks in the anterior-plating group and 12.7 weeks in the superior-plating group (P＞0.05),while the Constant Murley Score was 93.6 and 90.4 respectively (P＞0.05).The complications in the anterior-plating group were delayed union in 1 case,nonunion in 1 case,screw loosening in 2 cases,and hypertrophic scarring in 4 case.The complications in the superior-plating group were delayed union in 2 cases,plate breakage succeeding to nonunion in lcase,screw loosening in 3 case,and hypertrophic scarring in 7 case (P＞0.05).Conclusion:When treating complicated clavicle midshaft fracture with a reconstruction plate,there was no significant differences between anterior-plating and superior-plating.%目的:分析前置与上置重建钢板治疗锁骨中段复杂骨折的临床疗效.方法:回顾性分析88例闭合性锁骨中段复杂骨折采取重建钢板治疗的患者.钢板置于锁骨前方组共37例,男21例,女16例,年龄21～59岁,平均40.7岁.钢板置于锁骨上方组共51例,男38例,女13例,年龄19～62岁,平均42.5岁.随访14～35个月,平均19.3个月.对骨折愈合时间、并发症(延迟愈合、不愈合、内固定失效、肥大瘢痕)、Constant-Murley评分进行两组间比较.结果:钢板前置组和上置组的骨折愈合时间分别平均为11.8周和12.7周(P＞0.05),Constant-Murley评分分别平均为93.6分和90.4分(P＞0.05).钢板前置组的并发症为延迟愈合1例,不愈合1例,螺钉松动2例,肥大瘢痕4例.钢板上置组并发症为延迟愈合2例,不愈合继发钢板断裂1例,螺钉松动3例,肥大瘢痕7例.结论:采用重建钢板治疗锁骨中段复杂骨折时,在保证骨折断端两侧至3枚双皮质骨螺钉的情况下,钢板置于前方和置于上方对骨折愈合时间、并发症、Constant-Murley评分等临床疗效无明显影响.
  • 摘要：Objective:To evaluate the effect of the double capsule pressure infusion bag used in the patients with hemorrhagic shock.Methods:According to the inclusion/exclusion criteria,40 patients with hemorrhagic shock were randomly assigned to one of the two groups from January,2009 to June,2012.The Double capsule pressure infusion bag was used in the experimental group,while syringe with three-way pipe was used in the control group to increase the blood pressure.Results:The time for the infusion of 250 mL of the experimental group was much less than that of control group (P＜0.01),and the time of recovery of SBP/SaO2 of the experimental group was less than that of control group (P＜0.05),while the difference of the mean value of the systolic blood pressure (SBP),heart rate (HR) and the temperature of the two group after the infusion of 1000 mL had no statistical significance (P＞0.05).The survival rate of the two group had no statistical significance (P＞0.05).Conclusions:It was rapid and effective to increase the blood pressure in patients with hemorrhagic shock using the double capsule pressure infusion bag.%目的:评价应用双囊气压输液袋抢救失血性休克病人的疗效.方法:根据纳入/排除标准选择2009年1月至2012年6月收入我科的创伤失血性休克病人40例做为研究对象,分别采用双囊气压输液袋(实验组)或传统注射器配合三通管快速输液(对照组)恢复患者血压,观察和比较两种方法处理的患者输完250 mL液体所需的时间,恢复正常收缩压和正常动脉血氧饱和度的时间,以及连续扩容1000mL后的收缩压值、心率、体温和患者存活率.结果:实验组输注250mL液体所需的时间明显少于对照组(P＜0.01),实验组恢复正常收缩压和正常动脉血氧饱和度的时间少于对照组(P＜0.05),而两组患者在扩容1000 mL后收缩压、心率、体温的平均值的差异无统计学意义(P＞0.05),两组患者的存活率的差异无统计学意义(P＞0.05).结论:应用双囊气压输液袋补液抢救失血性休克病人能更迅速、有效恢复患者的收缩压.
  • 摘要：Objective:To investigate the therapeutic effects and security of remote anatomical plate surgery with inside and outside on comminuted supracondylar and intercondylar humerus fractures.Methods:80 patients with comminuted supracondylar and intercondylar humerus fractures were chosen in the period from March 2009 to May 2012,and randomly divided into two groups including Y-shaped plate group with Y-shaped plate treatment and double-plate group with remote anatomical plate surgery with inside and outside; and the excellent and good rate clinical improvement effects and the complication incidence of both groups were compared.Results:The excellent and good rate clinical improvement effects of double-plate group (100.0％)were significantly better than Y-shaped plate group (80.0％)(P＜0.05).And the complication incidence of double-plate group (5.0％) were significantly better than Y-shaped plate group (22.5％)(P＜0.05).Conclusion:Compared with Y-shaped plate,inner side and outside of the distal humerus the remote anatomical plate surgery with inside and outside on comminuted supracondylar and intercondylar humerus fractures possess excellent effects,can efficiently relieve the pain,and improve the activities scope of elbow,and reduce the incidence risk of complication.%目的:探讨内、外侧远端解剖钢板手术治疗肱骨髁上及髁间粉碎性骨折临床效果及安全性.方法:选取我院骨科2009年3月-2012年5月收肱骨髁上及髁间粉碎性骨折患者80例,采用随机数字表法分为Y型钢板组和双钢板组,每组各40例;分别采用Y型钢板内固定治疗和内外远端解剖钢板内固定治疗;比较两组患者术后临床改善优良率及术后并发症发生情况等.结果:Y型钢板组和双钢板组患者临床改善优良率分别为80.0％,100.0％;双钢板组患者临床改善优良率明显优于Y型钢板组,组间比较差异显著(P＜0.05);同时Y型钢板组和双钢板组患者术后不良并发症发生率分别为22.5％,5.0％;双钢板组患者术后并发症发生率明显低于Y型钢板组,组间比较差异显著(P＜0.05).结论:相较于Y型钢板,内、外侧肱骨远端解剖钢板手术治疗肱骨髁上及髁间粉碎性骨折临床效果确切,能够有效缓解疼痛,改善肘关节活动范围,同时术后并发症风险降低,具有较高临床应用价值.
  • 摘要：Objective:To investigate the expression and clinicopathological significance of angiopoietin-2 and α5β1 integrin in the breast cancer.Methods:180 cases of breast cancer and 80 cases of benign breast lesion admitted in Hunan Tumor Hospital from January 2005 to December 2011 were selected,the immunohistochemical method was applied to detect the Angiopoietin-2 and α5β1 Integrin expression,and relation of their expressions to survival and lymph node metastasis was analyzed.Results:The positive rates of Angiopoietin-2 expression in breast cancer and normal breast tissue were respectively 80.56％(145/180) and 11.25％(9/80),the positive rates of α5β1 Integrin expression in breast cancer and normal breast tissue were respectively 77.22％(139/180) and 16.25％(13/80),which were significantly higher in the breast cancer than those in the normal breast tissue (P＜0.01),but no significant correlationwas found between the expressions of Angiopoietin-2 andα5β1 Integrin in.The breast cancer (P＞0.05).The positive rate of Angiopoietin-2 expression was 97.93％(95/97) in breast cancer with lymph node metastasis and 60.03％(50/83)in breast cancer without lymph node metastasis,which was significantly higher in the breast cancer with lymph node metastasis than that in the breast cancer without lymph node metastasis (P＜0.05).However,the positive rate of α5β1 Integrin was 83.5％(81/97) in the breast cancer with lymph node metastasis and 69.87％ (58/83) in the breast cancer without lymph node metastasis,no,marked difference was found between them(P＞0.05).The survival time of Angiopoietin-2-negative and/or α5β1 Integrin-negative breast cancer were longer than Angiopoietin-2-positive and/or α5β1 Integrinpositive breast cancer (P＜0.01).Conclusion:The up-regulation of Angiopoietin-2 and α5β1 Integrin expressions may play an important role in the development of breast cancer and could be used as prognostic indicators for the evaluation of prognosis of patients with breast cancer.%目的:探讨Angiopoietin-2和α5β1 Integrin 在乳腺癌中表达及其临床病理学意义.方法:选择2005年1月至2011年12月湖南省肿瘤医院收治的180例乳腺癌病例和80例乳腺良性病变者为研究对象,应用免疫组织化学技术检测乳腺癌组织和乳腺良性病变者正常乳腺组织中Angiopoietin-2和α5β1 Integrin的表达,并分析其与患者的生存期及淋巴结转移的相关性.结果:Angiopoietin-2在乳腺癌和正常乳腺组织中的阳性表达率分别为80.56％(145/180)和11.25％(9/80),α5β1 Integrin在乳腺癌和正常乳腺组织中的阳性表达率分别为77.22％(139/180)和16.25％(13/80),乳腺癌中Angiopoietin-2和α5β1 Integrin的阳性表达率均显著高于在正常乳腺组织中的表达(P＜0.05),但乳腺癌组织中Angiopoietin-2和α5β1 Integrin 的表达无显著相关性(P＞0.05).有淋巴结转移及无淋巴结转移的乳腺癌中Angiopoietin-2的阳性表达率分别为97.93％(95/97)和60.03％(50/83),差异有统计学意义(P＜0.05);而有淋巴结转移及无淋巴结转移的乳腺癌中α5β1 Integrin的阳性表达率分别83.5％(81/97)和69.87％(58/83),差异无统计学意义(P＞0.05).Angiopoietin-2和/或α5β1 Integrin 阴性表达的乳腺癌患者生存期分别显著高于Angiopoietin-2和/或α5β1 Integrin阳性的乳腺癌患者(P＜0.01),差异有统计学意义(P＜0.01).结论:Angiopoietin-2和α5β1 Integrin 的表达上调可能在乳腺癌的发生发展过程中起重要作用,并可能作为预测乳腺癌患者预后的参考指标.
  • 摘要：目的:探讨糖尿病周围神经病变患者生活质量现状和影响因素.方法:采用自编问卷、抑郁自评量表(SDS)、焦虑自评量表(SAS)、社会支持评定量表(SSRS)、匹兹堡睡眠质量指数问卷(PSQI)和汉化版简明健康调查表(SF-36)量表对2010年10月～2012年10月在我院内分泌科住院治疗的50例糖尿病周围神经病变患者和同期50例在我院健康体检者进行测评,采用SPSS16.0软件进行分析找出糖尿病周围神经病变患者生活质量现状和影响因素.结果:糖尿病周围神经病变患者的生活质量明显低于健康体检者,有显著性差异(P＜0.05).多元线性逐步回归分析发现:文化程度高、患者对疾病了解多、社会支持评定量表总分高与糖尿病周围神经病变患者生活质量正相关,而慢性并发症种类多、有睡眠问题、焦虑抑郁标准分高与糖尿病周围神经病变患者生活质量负相关.结论:糖尿病周围神经病变患者生活质量较低,其生活质量受多方面因素的影响,我们应该采取针对性的干预措施来改善患者的生活质量.%Objective:To investigate the status of the quality of life and its influential factors of diabetic peripheral neuropathy patents.Methods:The clinical data of fifty diabetic peripheral neuropathy patients who were treated in the department of endocrinology in our hospital from October 2010 to October 2012 and fifty healthy control in the same period were collected,they were surveyed with questionnaire including self-made general questionnaire,Self-Rating Anxiety Scale,Self-Rating Depression Scale,Social Support Rating Scale(SSRS),Pittsburgh Sleep Quality Index (PSQI) and quality of life scale for diabetic peripheral neuropathy patients.Results:The index scores of all dimensions of quality of life scale for diabetic peripheral neuropathy patients in diabetic peripheral neuropathy patients were lower than those in the healthy control (P＜0.05).Multivariate stepwise regression analysis showed that degree of education,understanding degree of diabetic peripheral neuropathy,high scores of the social support rating scale were positive correlation with the quality of life in diabetic peripheral neuropathy patients,more kinds of chronic complications,having problems sleeping and high standard scores of anxiety and depression were negative correlation with the quality of life in diabetic peripheral neuropathy patients.Conclusion:The quality of life in patients with diabetic peripheral neuropathy were low,the influential factors of patients are affected by many factors,we should take the appropriate measures to improve the patient's quality of life.
  • 摘要：目的:建立CroweW型发育性髋关节脱位儿童骨盆三维有限元模型,对发育性髋关节脱位儿童真性髋臼及假性髋臼的生物力学进行初步分析.方法:采用单侧发育性髋关节脱位儿童骨盆CT扫描DICOM数据,通过Mimics10.0对图像DICOM数据进行重建,经Geomagic Proe5.0进行网格优化,在Hepermesh 10.0中进行有限元网格划分后输入ANSYS12.0中,在ANSYS中根据解剖部位建立骨盆主要韧带,行单腿站立载荷加载,计算该加载方式下骨盆的应力及位移分布情况.结果:模拟患者单腿(患侧)站立状态下身体重心通过假关节的中心,骨盆极度倾斜约45°,给予生理载荷,应力主要集中在假髋臼和骶髂关节面之间,耻骨上肢内侧是应力集中区但是应力小于骶髂关节周围部分;患侧骨盆位移以髂骨翼前侧向后侧逐渐减弱.结论:建立的有限元模型在静载荷下特征部位的应力及位移能够反映CroweⅣ型髋关节脱位儿童骨盆的力学结构特性,模型的准确性高,可以成为CroweⅣ型髋关节脱位儿童骨生物力学研究的工具,满足临床研究需要.%Objective:To construct a three-dimensional (3D) finite element pelvic model of child with Crowe Ⅳ developmental dislocation of the hip (DDH),and to investigate the stress around pseudo-acetabular and acetabular of DDH.Methods:One ten-year-old girl with DDH was scanned by multi-slices computerized tomography (MSCT) and the images of every cross-section were obtained.The three-dimensional images of the DDH child pelvis were reconstructed with the software Mimics 10.0.The three-dimensional model of the pelvis was imported into the ANSYS12.0 by the Geomagic Proe 5.0 and the Hempermesh 10.0.Digital three-dimensional structures of the DDH child pelvis,such as ligament,were added to the three-dimensional model with powerful pre-processing modular of ANSYS.Finally,the integrated three-dimensional finite elemental model of the DDH child pelvis was established.The boundary and loading conditions used Single-leg stance.The stress,strain and displacement nephogram were obtained under axial load.Results:Simulation patient standing on one leg with the physiological load,pelvis tilted by about 45°,and the distributions ofvon Mises stress was mainly between false acetabulum and sacroiliac joint surface.Pubic upper medial was also the stress concentration zone where the stress is less than the sacroiliac joint part.The distributions of displacement were from the iliac wing front side gradually weakened.Conclusions:The children pelvic finite element model of Crowe Ⅳ type development dislocation of the hip is valid and reasonable,which can be the biomechanics research tools to meet the needs of clinical research.
  • 摘要：Objective:To investigate the tolerance of children acute myeloid leukemia to high-dose cytarabine,so as to provide guidance for clinic.Methods:40 cases of acute myeloid leukemia patients admitted from January 2007 to January 2011 were selected as the clinical study objects,and randomly divided into group A and group B,each group of 20 patients.The two groups were given high-dose cytarabine.Patients of group A were given dosage of 3 g/m2 body surface area; group B were given dosage of 2 g/m2 body surface area.A week later observed was the therapeutic effect,nervous system toxicity and side effects of the two groups.Results:After one week of treatment,2 cases in group A (10.00％) had anemia,less than that in group B which had 8 cases of anemia (40.00％).Patients in group A showed severer degree m neurotoxic headache,lethargy,apathy and convulsions than in group B,as well as in aspects of nystagmus,dysdiadochokinesia and ataxia.Conclusion:High-dose of cytarabine has better efficacy and were generally tolerated,but resulted in severe adverse reactions.%目的:探讨儿童急性髓系白血病对于大剂量阿糖胞苷的耐受性,为临床用药提供指导.方法:从我院2007年1月～2011年1月收治的儿童急性髓系白血病患者中选取40例,将其作为临床研究对象,随机分成A、B两组,每组均20例患者.两组都给予大剂量阿糖胞苷,A组患者给予体表面积3 g/m2用量;B组患者给予体表面积2 g/m2用量.一周后,分别观察两组急性髓系白血病儿童的治疗效果、神经系统毒性和副作用.结果:治疗一周后,A组患者贫血例数2例(10.00％)较B组患者贫血例数8例(40.00％)少,A组患者所表现的神经毒性头疼、嗜睡、淡漠及惊厥方面较B组患者严重,A组患者所表现的副作用眼球震颤、轮替运动障碍、共济失调均较B组患者严重.结论:大剂量阿糖胞苷虽具有更好的疗效,基本耐受但其不良反应相对严重.
  • 摘要：Objective:Cell cycles of tumor cells' growth and development is closely related to a variety of regulatory factors of the abnormal.This essay is to study the Skp2 protein expression in the development process and the clinical pathological significance on the gingival carcinoma.Methods:46 cases with gingival cancer and 22 cases with normal gum tissue specimens were collected over the same period to prepare the tissue sections,and the Skp2 protein expression in the normal mucosa and gingival carcinoma with different pathological grades were detected by SP immunohistochemical methods.Results:There were statistical differences between the Skp2 protein expres-sion in normal oral mucosa tissue and gingival carcinoma,Skp2 in gingival cancer-positive rate (32.61％) was significantly higher than that in normal mucosa (4.55％) (x2=6.51,P＜0.05).The application of positive expression rate of the relationship of Skp2 with gingival cancer clinical stage,histological grade,gender and age.There were significantly associated between the Skp2 protein expression in nor-mal oral mucosa tissue and gingivai carcinoma (P＞0.05),but were unrelated with age,gender and other factors(P＞0.05).Conclusion:Skp2 protein in oral gingival cancer clinical grading and pathological grading is proportional to related.No significant correlation with age,gender,Skp2 may occur in the positive regulation effect plays in the development of important gingival carcinoma,detection of Skp2 is helpful to judge the malignant degree and prognosis of carcinoma of gingiva,provide a more reasonable scheme for clinical severity and treatment.%目的:肿瘤细胞的增殖和发展与多种细胞周期调控因子的异常密切相关,Skp-2通过泛素化降解p27,使肿瘤细胞G1-S期调控发生紊乱,从而参与肿瘤细胞的某些生物学性状,本实验探讨牙龈癌发生发展过程中Skp2蛋白的表达以及其与牙龈癌临床病理学之间的意义.方法:收集同期46例牙龈癌及22例正常牙龈组织标本,制备组织切片,应用SP免疫组化法检测正常黏膜及牙龈癌中Skp2蛋白的表达,以及Skp2蛋白在牙龈癌不同病理分级和临床分期中的表达.结果:Skp2蛋白在正常口腔粘膜组织与牙龈癌中的阳性表达率差异有统计学意义,Skp2蛋白在牙龈癌中的阳性率(32.61％,显著高于正常黏膜组织4.55 ％)(x2=6.51P＜0.05).应用阳性表达率表示Skp2与牙龈癌的临床分期、病理分级、性别、年龄之间的关系,结果显示Skp2与牙龈癌的临床分期及病理分级显著相关成正比例关系,差异具有统计学意义(P＜0.05),而与年龄、性别等因素不相关,无统计学差异(P＞0.05).结论:Skp2蛋白在口腔牙龈癌的临床分级和病理分级成正比例相关,与年龄、性别无显著相关,Skp2可能在牙龈癌的发生发展中起着重要的正向调控作用,检测Skp2有助于判断牙龈癌的恶性程度及其预后,为临床判断病变程度及治疗提供更加合理的方案.
  • 摘要：Objective:Exploration Pan Fushu joint acupoint application for the treatment of children with recurrent respiratory tract infections clinical efficacy.Methods:September 2009 September-2012 pediatric hospital treatment in 60 children with recurrent respiratory tract infections were randomly divided into two groups,the control group treated with conventional antibiotics and Point (Feishu,the smell of mutton,the days of the conflict,foot three Mile) patching (per 12 hours,every other day,15 times as a course of treatment) treatment; observation group on this basis,plus pan-Fusu treatment (3.5 mg daily fasting Dayton clothing,and even served 10 days,stopped 20 days for a course of treatment),patients were treated for three courses,the observation of the two groups were compared patient outcomes,immune indicators adverse reaction.Remults:The number of days of fever,cough on the number of days and the use of antibiotics was significantly less than that of the control group,the two groups was statistically significant (P ＜0.05); the immune indicators before and aiter treatment in the control group of various immune indicators before and after treatment did not change significantly,significantly elevated the observed serum IgG after treatment,with a statistically significant difference (P ＜0.05),IgA,elevated after treatment,but no significant differences largely unchanged,IgM after treatment.Conclusion:Pan Fushu combined with acupoint application for the treatment of children with recurrent respiratory tract infections significant clinical effect,treat the effective rate,has active clinical significance,worthy of promotion.%目的:探析泛福舒联合穴位贴敷对治疗儿童反复呼吸道感染的临床疗效.方法:选取2009年9月-2012年9月就诊于我院儿科的反复呼吸道感染的患儿60例随机分为两组,对照组给予常规抗生素加穴位(肺俞、膻中、天突、足三里)贴敷(每次12小时,隔日1次,15次为一个疗程)治疗;观察组在此基础上加用泛福苏治疗(3.5 mg,每天清晨空腹顿服,连服10天,停20天为一个疗程),两组均治疗3个疗程后,随诊6个月,观察对比两组患者治疗效果、免疫指标、用药不良反应、远期疗效等.结果:观察组在发热天数、咳嗽天数和抗生素使用时间上都明显少于对照组,两组相比具有统计学意义(P＜0.05);免疫指标治疗前后比较中,对照组各免疫指标治疗前后均没有明显改变,观察组血清IgG在治疗后明显升高,具有统计学差异(P＜.05),IgA在治疗后升高,但没有显著差异,IgM治疗后基本没有改变;观察组远期疗效明显优于对照组,两组相比具有统计学意义(P＜0.05).结论:泛福舒联合穴位贴敷对治疗儿童反复呼吸道感染临床效果显著,治疗有效率高,具有积极的临床意义,值得推广.
  • 摘要：目的:探讨超声在围绝经期妇科疾病诊断中的作用,为临床诊断提供依据.方法:选取2012年2月至2012年8月间于我院接受诊断和治疗的处于围绝经期的阴道不规则出血患者128例,观察超声诊断与病理学诊断的差异,分析超声诊断的作用.结果:超声诊断与病理学检查结果的总符合率为95.31％,内膜良性病变类型与内膜癌和癌前病变类型的平均内膜厚度均明显高于内膜生理变化类型(P＜0.05),子宫内膜厚度≥10mm的发生内膜良性病变或内膜癌及癌前病变的64例(66.67％),厚度＜10 mm的患者发生内膜良性病变或内膜癌及癌前病变10例(31.25％),差异有统计学意义(P＜0.05).结论:超声在围绝经期妇科疾病诊断中的作用显著,值得在临床上推广应用.%Objective:To discuss the effect of ultrasound diagnosis on perimenopausal women with gynecological diseases.Method:128 patients with irregular bleeding in our hospital were selected.The difference between ultrasound diagnosis and pathological examination was observed and the effect was analyzed.Results:The coincidence rate between ultrasound diagnosis and pathological examination was 95.31％.The average endometrial thickness of endometrial benign lesions type and endometrial cancer and precancerous lesions type were all thicker than the endometrial physiological changes type (P＜0.05).The rate of the group which endometrial thickness thicker than 10 um was 75.00 ％ and it was obviously higher than that of the group which endometrial thickness thinner than 10 mm (P＜0.05).Conclusion:The ultrasound diagnosis had a significant effect on perimenopausal women with gynecological diseases and deserved promotion.
  • 摘要：Objective:To investigate the Geriatric Hospital Acquired Pneumonia,resistance and prognostic risk factors.Methods:the etiology,susceptibility and prognostic risk factors of elderly patients with HAP received by our hospital in January 2007-2012 in December were analyzed.Results:Pseudomonas aemginosa,and E.coli had the highest detection rate in pathogenic G-bacteria,and the G cocci with the highest detection rate were Staphylococcus aureus and Streptococcus pneumoniae; all pathogens had certain resistances to antibiotics,the prognosis of patients Logistic analysis showed combined underlying disease,age,outside the city of population exposure to patients with pneumonia as well as smoking,alcohol consumption were risk factors affected the prognosis of patients with HAP.Conclusion:By the detection of pathogens and resistance in the elderly HAP analysis,to choice a reasonable of antimicrobial drugs to improve the prognosis of patients.%目的:探讨老年医院获得性肺炎病原学、耐药性以及预后危险因素.方法:对我院2007年1月-2012年12月收治的老年HAP患者进行病原学、药敏分析以及对预后的危险因素进行分析.结果:在G-杆菌中铜绿假单胞菌以及大肠埃希菌是检出率最高的致病菌,G+球菌中金黄色葡萄球菌以及肺炎链球菌检出率最高;所有致病菌均对常规抗生素均有一定的耐药性,对患者的预后的Logistic分析其中合并基础疾病、年龄、外市人口、接触肺炎患者以及吸烟、饮酒是影响HAP患者预后的危险因素.结论:对于老年HAP中进行检测病原菌以及耐药性进行分析,继而合理选择抗菌药物,提高患者的预后.
  • 摘要：Objective:To investigate the laparoscopic radical resection of rectal cancer peri-operation nursing.Methods:420 cases of colon cancer patients were chosen as the research object during December 2009 and December 2012,and all patients were randomly divided into two groups,group of laparoscopic operation and traditional open operation group,and each group consisted of 210 cases of rectal cancer patients.Various clinical nursing index detailed in two group was detected.Results:The ASA score before the operation,Dukes staging and operation time and other aspects in the laparoscopic operation and traditional open operation group were not statistically significant (P＞0.05).In addition,anal exhaust time,ambulation,or length of time and other aspects in the laparoscopic operation patients are less than that in the traditional open operation group of patients (P＜0.05),and intraoperative blood loss and postoperative complications were much less than that in the traditional open operation group (P＜0.05).Conclusion:The patients with rectal cancer,perform the treatment of laparoscopic operation with a faster postoperative recovery,shorter hospitalization time,fewer complications and clinical advantages.To strengthen the patients during peri-operation period of psychological care,to ensure clinical therapeutic effect,and to promote the recovery of the patients important method,is worthy of promotion and application of.%目的:研究腹腔镜直肠癌根治术围手术期的护理.方法:本组研究的对象为我院2009年12月-2012年12月期间共收治的420例结肠癌患者,并随机将所有的患者分为两组,腹腔镜手术组与传统开腹手术组,每组分别由210例直肠癌患者组成,将两组患者的各项临床护理指标进行详细的观察与分析.结果:腹腔镜手术组与传统开腹手术组患者在手术之前的ASA评分、Dukes分.期以及手术时间等方面均无统计学意义(P＞0.05).然而,腹腔镜手术组患者无论在肛门排气时间、下床活动时间,还是住院时间等方面均少于传统开腹手术组患者(P＜0.05,),且术中的出血量以及术后的并发症也明显少于传统开腹手术组患者(P＜0.05).结论:对于直肠癌患者而言,进行腹腔镜手术治疗具有术后恢复快,住院时问短,并发症少等临床优点.而加强患者的围手术期心理护理,则保障临床治疗效果,促进患者康复的重要手段,值得推广与应用.
  • 摘要：胃癌是消化系统最常见的恶性肿瘤之一,全球每年胃癌新发病例数接近百万,严重影响着人类健康.近年来医学领域发展迅速,不管是胃癌的手术方式,还是放疗、化疗等治疗手段均获得了较大提高.但是由于缺乏有效早期诊断方法,大多数胃癌患者确诊时已进入疾病晚期,所以尽管医学技术不断发展,胃癌的死亡率依然居高不下.随着对胃癌分子机制的不断深入研究,一种直接作用于胃癌主要信号转导通路上某一特定靶点的新型治疗方式-分子靶向治疗逐渐成为当前肿瘤研究的热点.目前已有多种单克隆抗体及小分子靶向药物进入临床,用于人体多种肿瘤的治疗并呈现较好疗效.分子靶向治疗的出现为进展期肿瘤患者带来了新的希望.本文主要就进展期胃癌分子靶向治疗现状以及最新研究进展作一综述.%Gastric carcinoma,one of the most frequent malignancies of digestive system,has a seriously influence on human health.Nearly one million new cases are diagnosed each year.With the fast development of medicine,not only remarkably advances in surgical techniques,but also in chemoradiotherapy over the past few years.However,due to lack of clearly defined technique on early diagnosis,the majority of GC patients are diagnosed at an advanced stage of disease,the prognosis for GC patients with late-stage disease remains poor.With emerging understanding of the molecular pathways about GC,a new method-molecular targeted therapy that designed to interfere with specific molecular events in the key pathways of tumor growth and progression,has become the primary focus of therapeutic cancer research.Now there are some monoclonal antibodies and small molecular targeted drugs have been used to treat tumors of human.The clinical trails,to some extent,find that patients can benefit from targeted therapy which brings new hope for the patients with advanced cancer.In this review we describe the current status of targeted therapies in the treatment of GC.
  • 摘要：Objective:To investigate the expression of SUFU,GLI1 gene in normal pancreatic tissue,pancreatic cancer and adjacent carcinoma tissue.To analyze the expression ofmiR-223 and the relationship with the GLII,SUFU gene transcription,as well as the effect of SUFU and GLI1 gene on the pathogenesis of pancreatic cancer.Methods:To detect the SUFU and GLI1 gene transcription by the extraction of total RNA in the normal pancreatic tissue,pancreatic cancer tissue and cancer adjacent tissues with the method of Real time PCR,and to analyze the mRNA expression of SUFU and GLI1,as well as the correlation of them.Results:GLIlmRNA expression in pancreatic cancer tissue is 4.79 (1.19,9.89),the amount of expression in the tissue adjacent to carcinoma of pancreatic cancer was 2.01 (0.70,5.76)(P＜0.05).SUFU in pancreatic cancer tissue mRNA expression quantity of 1.34 (0.91,2.31),the expression of pancreatic tissue adjacent to carcinoma amount was 1.51 (1.23,2.56)(P＞0.05),pancreatic tissue adjacent to carcinoma in GLI1 mRNA expression and miR-223 in pancreatic cancer tissue adjacent to carcinoma existed a certain degree of correlation between the expression amount (P＜0.05).Conclusion:GLIlmRNA participated in the incidence of pancreatic cancer,and SUFU mRNA has lit-tle to do with pancreatic cancer.GLI1 gene and miR-223 in the development of pancreatic cancer may play a synergistic action.%目的:探明SUFU、GLI1基因在正常胰腺组织以及胰腺癌和癌旁组织中的mRNA表达,分析miR-223的表达与GLI1、SUFU基因转录之间的关系,以及SUFU、GLI1基因在胰腺癌发病过程中的作用.方法:对正常胰腺组织以及胰腺癌和癌旁组织进行总RNA的提取,采用Real-time PCR方法,检测SUFU、GLI1基因的转录情况,对SUFU、GLI1的mRNA的表达量与miRNA-223的表达量进行相关性分析.结果:胰腺癌组织中GLI1 mRNA的表达量为4..79 (1.19,9.89),胰腺癌癌旁组织中的表达量为2.01(0.70,5.76)(P＜0.05).胰腺癌组织中SUFU mRNA表达量为1.34 (0.91,2.31),胰腺癌癌旁组织的表达量为1.51(1.23,2.56)(P＞0.05),胰腺癌癌旁组织中GLI1 mRNA的表达量与miR-223在胰腺癌癌旁组织中的表达量之间存在一定程度的相关性(P＜0.05).结论:GLI1mRNA参与了胰腺癌的发生,而SUFU mRNA与胰腺癌发生关系不大.GLI1基因及miR-223在胰腺癌的发生中可能起到一定的协同作用.
  • 摘要：目的:比较电视胸腔镜手术与传统手术方法对非小细胞肺癌治疗的临床效果.方法:选择2010年5月至2012年6月本院收治的非小细胞肺癌患者84例,随机分为VATS组和OT组,各42例,VATS组行全胸腔镜肺叶切除术;OT组行传统开胸肺叶切除术.比较两组患者手术时间、淋巴结清扫数、术中出血量、术后引流时间、住院天数、术后疼痛和并发症发生情况,观察并比较两组患者术后3d血清CRP、TNF-α、IL-6、IL-10水平.结果:VATS组术后引流时间、住院时间明显短于OT组(P＜0.05);两组手术时间、淋巴结清扫数、术中出血量及并发症发生率比较无统计学差异(P＞0.05);VATS组术后1d、3d疼痛评分明显低于OT组(P＜0.05);VATS组术后3d血清CRP、TNF-α、IL-6、IL-10水平明显低于OT组(P＜0.05).结论:与传统手术相比,电视胸腔镜手术对患者创伤较小,患者术后炎症反应较低,痛苦较小,可作为非小细胞肺癌的推荐手术方式.%Objective:To compare the clinical effect of video-assisted thoracoseopic surgery and traditional operation method in non-small cell lung cancer.Methods:84 patients with non small cell lung cancer who were treated in our hospital from May 2010 to June 2012 were selected and randomly divided into VATS group and OT group,42 cases in each group.Patients in the VATS group were underwent video-assisted thoracoscopic surgery,while patients in the OT group were underwent thoracotomy combined with lobectomy.The resected lymph nodes,operation time,inttroperative bleeding volume,postoperative drainage time,hospitalization,postoperative pain and complications of patients were compared between two groups,and the postoperative 3 d serum CRP,TNF-α,IL-6 and IL-10 levels were detected.Results:The drainage time and hospitalization of patients in the VATS group was significantly shorter than those of the OT group (P＜0.05); There was no statistically significant difference about the operation time,intraoperative lymph node yield,the blood loss and the incidence of complications between two groups (P＞0.05); The pain of patients in the VATS group was significantly lower than that of the OT group after treating for one day and three days(P＜0.05); The CRP,TNF-α,IL-6,IL-10 levels of patients in the VATS group were significantly lower than those in the OT group after three days (P＜0.05).Conclusion:Compared with the traditional operation,small video-assisted thoracoscopic operation on patients can be used as a non small cell lung cancer with the advantages of mix trauma,less postoperative inflammatory reaction and less pain.
  • 摘要：目的:观察并对比二肽基肽酶-Ⅳ抑制剂西格列汀、胰岛素增敏剂罗格列酮分别联合降糖药物二甲双胍治疗老年2型糖尿病(T2DM)的临床疗效.方法:入选2010年4月-2012年10月间我科收治的老年T2DM患者70例,并随机单盲分为A(n=36)、B(n=34)两组,A组患者予西格列汀+二甲双胍方案,B组予罗格列酮+二甲双胍方案,服药12周后对比两组血糖水平、临床疗效并药物不良反应.结果:①两组患者服药12周后,血糖指标均较治疗前明显下降(P＜0.05),A组患者2hPG水平明显低于B组水平(P＜0.05).②A组患者显效率(50.0％)、总体有效率(91.7％)略高于B组(38.2％、85.3％),差异不具有统计学意义(P＞0.05).③两组患者均未出现严重药物不良反应,两组不良反应发生率(19.4％vs.26.5％)无统计学差异(P＞0.05).结论:两种用药方案均是治疗老年T2DM的有效方案,西格列酮+二甲双胍方案较之罗格列酮+二甲双胍方案在临床疗效中具备比较优势,尤其是对降低餐后血糖优势明显.%Objective:To observe and compare the clinical efficacy between sitagliptin and the rosigiitazone combined with mefformin respectively on the treatment of type 2 diabetes in elder patients.Methods:70 admitted cases from Apr.2010 to Oct.2012 were randomized into A (n=36) & B (n=34)group single blindly,group A was given sitagliptin plus metformin schme,and group B was applied with rosiglitazone plus metformin schme,both were treated for 12 weeks,After which blood glucose level,clinical efficacy and adverse drug reaction during treatment were noted and compared between 2 groups.Results:(①)Glycemic indexes of 2 group patients after 12 weeks'therapy were lower than pre-treatment (P＜ 0.05),2hPG level of group A was significantly lower than that of group B (P＜ 0.05).(②)Patients in group A with significant efficiency (50％),overall efficiency (91.7％) was slightly higher than that of group B (38.2％,85.3％),the difference was not statistically significant (P＞0.05).③There were no seriously adverse drug reactions in 2 groups,incidences of adverse reactions of 2 groups(19.4％vs.26.5％) showed no significant difference (P＞0.05).Conclusion:Both 2 schemes are effective in treatment of elderly patients with type 2 diabetes mellitus,sitagliptin plus metformin scheme compared with rosiglitazone plus metformin scheme has a comparative advantage in clinical efficacy,and especially has obvious advantage in reducing postprandial blood glucose.
  • 摘要：目的:探讨右心室间隔部(Right Ventricular Septum,RVS)起搏和右心室心尖部(Rightventricularape,RVA)起搏对心功能的影响,为临床提供参考.方法:采用前瞻性研究的方法,对我院自2008年8月-2011年8月收治的行起搏器治疗的72例患者随机均分为实验组和对照组,实验组采用RVS起搏,对照组采用RVA起搏,比较植入后15分钟和1年后测定两组间心室起搏参数差异及血流动力学参数左室射血分数(LVED、每搏量(sv)、心脏指数(CD、二尖瓣血流E峰和A峰最大充盈速度比值(E/A)差异.结果:植入后15分钟和1年后,实验组和对照组起搏参数起搏阈值、电极阻抗、心腔内R波幅度进行比较,差异无统计学意义(P＞0.05).植入后1年后,两组间的血流动力学参数比较,差异有统计学意义(P＜0.05).结论:RVS起搏优于RVA起搏,有望替代传统的右心室心尖部成为最佳的心室起搏部位.%Objeodve:To explore cardiac function influence on Right Ventricular Septum pacing and right ventricular apex pacing,it provide reference for clinical.Methods:By the prospective study method,72 patients with pacemaker treatment were randomly divided into the experimental group and the control group from August 2008 to August 2011,experimental group have RVS pacemaker,control group use RVA pacemaker implantation,after 15 minutes and 1 years later measurement between the two groups ventricular pacemaker parameter difference hemodynamic parameters and left ventricular ejection fraction (LVEF),each stroke volume (sv),cardiac index (CI),mitral valve flow E peak and A peak maximum filling speed ratio (E/A) were compared.Results:After 15 minutes and 1 years later,the experimental group and the control group pacemaker parameter pacemaker threshold,the electrode impedance,heart cavity R wave amplitude were compared,the difference was not statistically significant (P＞0.05).One year after implantation,between the two groups were compared in hemodynamic parameters,their difference was statistically significant (P＜0.05).Conclusion:RVS pacemaker is better than the RVA pacemaker,it is expected to replace the traditional right ventricular apex department,and it is the best ventricular pacemaker parts.
  • 摘要：Objective:To study the clinical effect of the anal minimally invasive surgical treatment of pediatric congenital hirschsprung disease.Methods:40 eases of congenital megacolon children,were randomly divided into control group and observation group.The control group in the abdominal perineal joint surgery treatment,and the observation group used minimally invasive surgical treatment of anal.Analysis operation time,intraoperative amount of bleeding,restore bowel evacuation time,length of hospital stay,complications,etc of two groups.Results:The amount of bleeding,intestinal recovery evacuation time,length of hospital stay of the observation group were significantly superior to control group,the difference was statistically significant (P＜0.05).The observation group operation time and control group was statistically significant difference (P＞0.05).The observation group complication rates significantly lower than those of the control group,the difference was statistically significant (P＜0.05).The observation group postoperative bowel movement control function is significantly better than control group,the difference was statistically significant (P＜0.05).Conclusions:The anal minimally invasive surgical treatment of pediatric congenital hirschsprung disease,curative effect is better than the abdominal perineal joint surgery,postoperative patients defecation function recovered well,operation condition good,complications,and less in,it is worth clinical application.%目的:探讨经肛门微创手术治疗小儿先天性巨结肠症的临床效果.方法:将我院收治的40例先天性巨结肠患儿,随机分为对照组和试验组,对照组采用经腹会阴联合手术治疗,试验组采用经肛门微创手术治疗.分析两组手术时间、术中出血量、恢复肠道排气时间、住院时间,并发症发生情况等.结果:组术中出血量、肠道恢复排气时间、住院时间均显著优于对照组,差异有统计学意义(P＜0.05).试验组手术时间与对照组差异无统计学意义(P＞0.05).试验组并发症发生率低于对照组,差异有统计学意义(P＜0.05).试验组术后排便控制功能显著优于对照组,差异有统计学意义(P＜0.05).结论:采用经肛门微创手术治疗小儿先天性巨结肠症,术后患者排便功能恢复好,手术情况佳,并发症少,疗效优于经腹会阴联合手术,值得临床推广应用.
  • 摘要：目的:研究联合应用重组人甲状旁腺素(1-34)和双磷酸盐对双侧卵巢切除(OVX)骨质疏松大鼠模型的治疗作用.方法:选用3月龄健康SD雌性大白鼠40只作为实验动物,随机分为五组:假手术组(S组)、卵巢切除组(o组)、OVX +RhPTH(1-34)组(R组)、OVX+阿仑磷酸钠组(A组)、OVX+联合用药组(RA组).术后12周开始给药,持续给药6周.给药后6周取血行相关生化指标检测,取股骨进行骨密度测定、骨组织形态计量学分析.结果:O组的大鼠股骨骨密度值较假手术组有明显减少,血清磷、骨钙素和血清雌二醇水平减少(P＜0.01),血清碱性磷酸酶水平增加(P＜0.01),使用甲状旁腺素和阿仑膦酸钠治疗后,大鼠股骨骨密度值、血清磷、骨钙素和血清雌二醇水平增加,血清碱性磷酸酶水平相应下降,尤其是两种药物联合应用后效果更为明显(P＜0.01);荧光双标显示,O组骨形成速率较R组及RA组低(P＜0.05),S组、O组、A组骨形成速率无明显差别(P＞0.05).结论:联合应用RhPTH(1-34)和双磷酸盐可以减少去卵巢大鼠骨量的丢失,预防骨质疏松的发生.%Objective:To investigate the effect of sequential application of rhPTH1-34 and bisphosphonates on osteoporosis in ovariectomized rats in order to provide evidence for its clinical application.Methods:Forty SD female rats at the age of 3 months were randomly divided into 5 groups:sham operation group (S group),ovariectomized group (O group),ovariectomized +RhPTH1-34 (R group),ovariectomized +Alendronate Sodium (A group),ovariectomized +RhPTH1-34 and Alendronate Sodium (RA group).The rats were given drugs after 12 weeks.Bone mineral density (BMD),biochemical indexes,and bone histomorphometry parameters were measured with computer image analysis in tetracycline bone double labeling fluorescence were measured after treatment 6 weeks later.Results:The bone mineral density of femur and concentration of P iron,E2,BGP in group O decreased significantly,and concentration of ALP increased significantly compared with S group (P＜0.01).RhPTH1-34 and Bisphosphonates improved BMD and biochemical indexes especially in RA group.The tetracycline bone double labeling fluorescence show that the mineral apposition rate in O group was lower than those in R and RA group.There were no significant differences of MAR among group S,O and A group (P＞0.05).Conclusion:Sequential Application of rhPTH1-34 and bisphosphonates was effective in treating osteoporosis in ovariectomized rats,especially in improving BMD of femur.
  • 摘要：目的:肺癌是严重危害人类健康的恶性肿瘤之一,中晚期肺癌以放化疗为主,毒副作用大,观察低毒副作用的人参皂苷Rg3和苏拉明联合对小鼠Lewis肺癌生长转移的抑制作用及机制,为临床安全高效治疗肺癌提供实验资料.方法:将40只接种好Lewis肺癌细胞的C57BL6小鼠随机分为5组,每组8只:A组:对照组、B组:顺铂组、C组:人参皂苷Rg3组、D组:苏拉明组、E组:人参皂苷Rg3联合苏拉明组.接种第4天后开始药物干预,第20天处死,取肺组织,计肺转移结节数,剥离.移植瘤,计瘤重,计算抑瘤率,通过RT-PCR及Western-blot检测移植瘤TGF-β1的mRNA及蛋白的表达.结果:各药物干预组的肺转移结节数及瘤重明显低于对照组(P＜0.05,P＜0.01),人参皂苷Rg3联合苏拉明组最明显;BCDE各组抑瘤率分别为:(19.69±5.61)％、(35.37±8.97)％、(35.85±7.55)％和(49.39±6.99)％,ABCDE各组的肺转移率分别为100％、100％、100％、87.5％和62.5％;各药物干预组的TGF-β1的mRNA及蛋白的表达均低于对照组(P＜0.01),且人参皂苷Rg3联合苏拉明组TGF-β1的表达明显低于人参皂苷Rg3组和苏拉明组(P＜0.05).结论:人参皂苷Rg3联合苏拉明具有抑制小鼠Lewis肺癌的生长转移作用,对晚期肺癌的抑制可能与下调TGF-β1的表达有关.%Objective:Lung cancer is a malignant tumor serious endangering human health.Radiotherapy and chemotherapy is the main treatment in metaphase or terminal lung cancer,but it has great toxic side effects.The aims of this study is to observe the inhibitory efficacy of Ginsenoside Rg3 in combination with Suramin on the growth and the metastasis of the Lewis lung cancer (LLC) in mice and the potential mechanisms,and supply the experiment data for the lung cancer clinical treatment with efficacy and safety.Methods:Highly metastasis Lewis lung cancer cells were inoculated into 40 C57BL6 mice to establish lung cancer models.The fourth day after inoculation,the mice were randomized into 5 groups (group A,B,C,D and E),and received normal saline alone,cisplatin,ginsenoside Rg3,suramin,ginsenoside Rg3 combined with suramin therapy respectively for 16 days.After treatment completed,the metastasis nodules on lung surface and the transplantation tumor weight were measured in every group,and transforming growth factor-β1 (TGF-β1) was detected by RT-PCR and western blot.Results:In cisplatin,suramin,ginsenoside Rg3,ginsenoside Rg3 combined with suramin groups,the transplantation tumor weight was suppressed obviously,with the tumor inhibitory rate of (19.69± 5.61)％,(35.37± 8.97)％,(35.85± 7.55)％ and (49.39± 6.99)％,respectively (P＜0.01).The lung metastasis nodules were also decreased significantly in these therapy groups,especially in the combination group (P＜0.05).The metastasis rate on lung surface was 100％,100％,100％,87.5％ and 62.5％ in group A,B,C,D and E respectively.The expression levels of TGF-β 1 mRNA and protein in the transplantation tumor both was down-regulated in cisplatin,suramin,ginsenoside Rg3,ginsenoside Rg3 combined with suramin groups than in control group,and was also obviously in combination group (P＜0.05).Conclion:Ginsenoside Rg3 in combination with Suramin can inhibit effectively the growth and metastasis of LLC mice,which is involved in the suppression of TGF-β1 expression.
  • 摘要：Objective:To investigate the correlation of death-associated protein kinase (DAPK) methylation and HPV infection with cervical lesions in Xinjiang Uighur,and provide references for the diagnosis and treatment of cervical lesions.Methods:30 cases ofchronic cervicitis,30 cases of CIN Ⅰ,30 cases of CIN Ⅱ/Ⅲ and 30 cases of cervical squamous cell carcinoma were selected,the methylation levels of DAPK were detected by MSP method and HPV infecion were determined by PCR,the relationship between which were analyzed.Results:(l)The methylation rate ofDAPK gene in chronic cervicitis,cervical intraepithelial neoplasia Ⅰ (CIN Ⅰ),CIN Ⅱ /Ⅲ and invasive squamous carcinoma of cervix were respectively 3.33％,10％,36.7％,63.3％.The methylation rate of DAPK in the SCC group was significantly higher than those in the other three groups (P＜0.05).(2)The positive rate of HPV infection in four groups were respectively 43.3％,56.7％,6.7％,93.3％,which was significantly higher in the SCC group than those in the other three groups(P＜0.05).(3)The level of methylation ofDAPK gene in cervical lesions and positive rate of HPV-DNA was positively correlated.Conclusion:The methylation of DAPK gene promoter and HPV infection played an important role in the development of cervical lesions in Xinjiang Uighur women,especially squamous cell carcinoma of the cervix.The methylation of DAPK gene promoter and HPV infection were positively correlated,combined detection of which may contribute to the diagnosis of squamous cell carcinoma of the cervix in Xinjiang Uighur women.%目的:探讨死亡相关蛋白激酶(death associated protein kinase,DAPK)甲基化及人乳头瘤病毒(humanpapillomavirus,HPV)感染与新疆维吾尔族妇女宫颈病变的相关性,为维吾尔族妇女宫颈病变的诊断和治疗提供参考依据.方法:选择新疆维吾尔自治区人民医院2008年至2009年收治的宫颈非癌组织30例、宫颈上皮内瘤变(CIN)Ⅰ 30例、CIN Ⅱ/Ⅲ30例、宫颈鳞癌组织30例患者的组织标本为研究对象,运用MSP法检测宫颈组织中DAPK基因启动子甲基化水平及PCR检测各组织中HPV的感染情况,对二者进行相关性分析.结果:(1)宫颈非癌组织、CINⅠ、CIN Ⅱ/Ⅲ、宫颈鳞癌组织中DAPK基因甲基化的检出率分别为3.33％、10％、36.7％、63.3％,宫颈鳞癌组的甲基化率明显高于其它三组,差异有统计学意义(P＜0.05);(2)四组HPV的阳性率分别为43.3％、56.7％、76.7％、93.3％,宫颈鳞癌组的 HPV感染率明显高于其它三组,差异有统计学意义(P＜0.05),HPV的感染率和宫颈病变程度成正相关(P＜0.05).(3)宫颈病变组织中DAPK基因的甲基化水平与HPV-DNA阳性率呈正相关.结论:DAPK基因启动子的甲基化及HPV感染在新疆维吾尔族妇女宫颈病变尤其是宫颈鳞癌的发生过程中可能起重要作用,二者呈正相关,联合检测二者可能有助于新疆维吾尔族妇女宫颈鳞癌的诊断.
  • 摘要：Objective:To investigate the changes of renal interlobular arterial resistive index (RI) in patients with primary hypertension,and research what renal arterial RI means at patients with hypertension.Methods:115 cases of primary hypertensive patients were selected.Renal arterial RI were detected in all cases by Color Doppler Flow Imaging.Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured on the same day of examination.Pulse pressure (PP),Mean pressure(MBP) was calculated.Blood urea nitrogen (BUN),serum creatinine (Scr) etc were measured in all Patients.Results:Renal interlobular arterial RI in hypertension patients showed that renal arterial RI positively correlated with Hypertension time,SBP,PP,age Ccr and negative correlated with DBP.No correlated with MBP BUM and Scr.Conclusion:Primary hypertensive patient's renal interlobular arterial RI correlated with Hypertension time,SBP DBP PP AGE Ccr,Renal interlobular arterial RI can reflect the state of arteriosclerosis in the body.%目的:分析原发性高血压患者小叶间肾动脉阻力指数(resistive index,RI)与临床指标的相关性,研究肾动脉阻力指数对高血压患者临床意义.方法:选择原发性高血压患者115例.用彩色多普勒超声仪测量肾小叶间动脉的RI,彩超检查的当天测量收缩压(SBP)和舒张压(DBP),计算脉压(PP)、平均血压(MBP);检测血清尿素氮(BUN)、血清肌酐(Scr)、血清尿酸(UA),估算内生肌酐清除率(Ccr);分析小叶间肾动脉RI与临床指标的相关性.结果:高血压患者小叶间肾动脉RI与Ccr、SBP、DBP、PP、年龄、高血压发病时间均有相关性.高血压分级、平均血压、BUN、Scr、UA与小叶间肾动脉RI无相关性.结论:原发性高血压患者的小叶间肾动脉RI与Ccr、SBP、DBP、PP、年龄、高血压发病时间相关,可以间接反映高血压对肾动脉的影响及全身动脉硬化的状况.
  • 摘要：目的:研究HPV-18病毒E2、E6与Brd4对宫颈癌及癌前病变的意义.方法:选择2009年3月至2011年3月我院接诊的17例宫颈炎患者,19例CIN Ⅰ级(轻度非典型增生)患者,14例CIN Ⅱ级(中度非典型增生)患者,15例CIN Ⅲ级(重度非典型增生及原位癌)患者,19例宫颈癌患者.分别采用RT-PCR对各组的E2 mRNA、E6mRNA阳性表达情况进行测定,采用蛋白印迹(Western Blot)法对各组患者的E2蛋白与Brd4表达情况进行测定.从而分析HPV-18病毒E2、E6与Brd4对宫颈癌及癌前病变的临床意义.结果:各项检测后,发现宫颈癌组患者的E2mRNA阳性表达率明显低于宫颈炎组、CIN组(P＜0.05);宫颈癌组患者的E6mRNA阳性表达率明显高于宫颈炎组、CIN组(P＜0.05).HPV-18病毒E2 mRNA与E6mRNA阳性表达情况呈负相关(P＜0.05).宫颈癌组患者的E2蛋白阳性率明显低于宫颈炎组、CIN组(P＜0.05);宫颈癌组患者的Brd4蛋白阳性率明显低于宫颈炎组、CIN组(P＜0.05).结论:HPV-18病毒E2、E6与Brd4对宫颈癌及癌前病变的检测有重要意义,可用于对于宫颈癌演变过程的监控.%Objective:To study the expression of human papillomavirus-18 E6,E2 and cellular bromodomain protein Brd4 in cervical intraepithelial neoplasia and cervical carcinoma.Methods:The patients from March 2009 to March 2011 were studied,there were 17 patients with cervicitis patients,19 cases ofCIN Ⅰ (mild dysplasia),14 patients ofCIN Ⅱ level (moderate dysplasia) patients,15 cases ofCIN Ⅲ level in patients with (severe dysplasia and carcinoma in situ),19 cases of cervical cancer.RT-PC R were used for each group E2 mR NA,E6 mR NA positive expression was measured using western blot (Western Blot) method of the E2 protein and Brd4 expression of the patients in each group were measured.To analyze the clinical significance of the HPV-18 virus E2,E6 the Brd4 of cervical cancer and precancerous lesions.Results:After tested,we found that the E2 mRNA positive expression rate of patients with cervical cancer group was significantly lower than cervicitis group and the CIN group (P＜0.05); positive expression rate of cervical cancer patients E6 mRNA cervicitis group was significantly higher than cervicitis group and CIN group (P＜0.05).HPV-18 virus E2 mRNA E6 mR NA-positive expression was negatively correlated (P＜0.05).The E2 protein of cervical cancer group was significantly lower than cervicitis group and CIN group (P＜0.05); Brd4 protein positive rate of cervical cancer patients was significantly lower than the cervicitis group and CIN group (P＜0.05).Conclusion:HPV-18 virus E2,E6 and Brd4 detection of cervical cancer and precancerous lesions is important,they can be used to monitor the process of cervical cancer acted.
  • 摘要：Objective:To investigate the effection and the adverse reactions of ropivacaine and ketamine in neonatal caudal block.Methods:From 45 patients who will be suffered caudal block,we divided them into A,B,C three groups randomly and each group have 15 people.The patients of group A will be injected in caudal block by ropivacaine,the patients of group B will be injected in caudal block by ropivacaine and ketamine subcutaneous injection,the patients of group C will be injected in caudal block by the miscible liquids of ropivacaine and ketamine.After the operation,we record the patients' pain and analgesic time in 1,2,4,6,8,12,24hour.FLACC method will be used to evaluate analgesic effect,and taking the score＜4 points as an effective analgesic time time.Results:Comparing the analgesic time and cases different point in time without pain patients of group A and group B,we find that no bigger difference between the two groups(P＞0.05).Compared with A,B two groups,the analgesic time of group C seems longer significantly.And after 4,6,8,12,24 h patients there are more patients who don't need analgesic increased obviously (P＜0.05).However,three groups of patients were not shown strong adverse reaction.Conclusion:Using ropivacaine and ketamine in neonatal caudal block effects better.%目的:探明罗哌卡因、氯胺酮混合液在新生儿骶管阻滞术后镇痛的疗效和不良反应.方法:选取45例即将行骶管阻滞的新生儿,随机分成A、B、C三组,每组15人,其中A组患者行罗哌卡因骶管阻滞,B组患者行罗哌卡因骶管阻滞并氯胺酮皮下注射,C组患者行罗哌卡因、氯胺酮混合液骶管阻滞.术后于1、2、4、6、8、12、24h分别记录各患者的镇痛情况以及镇痛时间,采用FLACC法评价镇痛效果,以评分＜4分的维持时间作为有效镇痛时间.并且观察术后48h内不良反应的发生情况.结果:A组与B组之间比较镇痛时间以及术后不同时间点不需要镇痛的患者例数,发现两组间并没有较大差别(P＞0.05).相比较于A、B两组,C组患者阵痛时间明显延长,并且术后4、6、8、12、24h患者不需要镇痛明显增多(P＜0.05).而在不良反应方面,三组患者均未表现出过强的不良反应.结论:罗哌卡因、氯胺酮混合液用于新生儿骶管阻滞效果较佳,且不良反应少.

    现代生物医学进展的期刊信息

    • 曾用名：中华生物磁学;生物磁学
    • 创刊时间：2001
    • 地区：CN
    • 语言：中文
    • 热门主题：黑龙江省森工总医院;哈尔滨医科大学附属第四医院
    • 学科分类：

    现代生物医学进展的获奖情况

    现代生物医学进展的收录情况

    • • 文摘杂志
    • • 农业与生物科学研究中心文摘
    • • 化学文摘（网络版）
    • • 哥白尼索引
    • • 乌利希期刊指南
    • • 中国科技核心期刊

    现代生物医学进展的投稿信息

    • 投稿方式：
    • 投稿须知：点击查看
    • 期刊官方网站：点击前往
    • 版面费：
    • 审稿费：元
    • 通讯地址：哈尔滨市54号信箱（(哈尔滨市花园街184号403）
    • 邮编：150001
    • 联系电话：0451-82583800 53658268
    • 邮箱：biomed_54@126.com;liudhui_21@126.com；biomagnetism@
    • 期刊栏目：综述报告；学术探讨；学术研究；科技前沿；实验研究；磁疗病例；磁医学；磁疗保健
    免费订阅本刊

    客服邮箱：kefu@zhangqiaokeyan.com

    京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有 出版物经营许可证

    • 客服微信

    • 服务号