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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >L-NAME prevents GM1 ganglioside-induced vasodilation in the rat brain.
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L-NAME prevents GM1 ganglioside-induced vasodilation in the rat brain.

机译：L-NAME可防止GM1神经节苷脂诱导的大鼠脑血管舒张。

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摘要

Monosialoganglioside (GM1) is a glycosphingolipid present in most cell membranes that displays antioxidant and neuroprotective properties. It has been recently described that GM1 induces vasodilation. However, the mechanisms underlying GM1-induced vasodilation were not evaluated to date. Therefore, in this study we investigated whether the nonspecific NOS inhibitor l-NAME prevents GM1-induced vasodilation in rats. The systemic injection of GM1 (50mg/kg, i.p.) increased the outer diameter of pial vessels by 50% in anesthetized animals at 30min, and this effect was fully prevented by the administration of the nitric oxide synthase inhibitor N(G)-nitro-l-arginine methyl ester (l-NAME, 60mg/kg, i.p. 15min before GM1 injection). A 30min exposure of cerebral cortex slices to GM1 (100microM) increased the content of nitrite plus nitrate (NOx) by 50%. Addition of l-NAME (100microM) to the incubation medium fully prevented GM1-induced NOx increase. Conversely, a 60min exposure of slices to GM1 (100microM) decreased NOx content, revealing a biphasic effect of GM1. Our results suggest that NO plays an important role in the vasodilation induced by GM1.
机译：单唾液酸神经节苷脂（GM1）是存在于大多数细胞膜中的糖鞘脂，具有抗氧化和神经保护特性。最近已经描述了GM1诱导血管舒张。但是，迄今为止尚未评估GM1诱导的血管舒张的潜在机制。因此，在这项研究中，我们调查了非特异性NOS抑制剂1-NAME是否能阻止GM1诱导的大鼠血管舒张。全身注射GM1（50mg / kg，ip）在30分钟时可使麻醉动物的乳头血管外径增加50％，并且通过使用一氧化氮合酶抑制剂N（G）-硝基- l-精氨酸甲酯（l-NAME，60mg / kg，在GM1注射前腹腔注射15分钟）。大脑皮层切片暴露于GM1（100microM）30分钟后，亚硝酸盐和硝酸盐（NOx）的含量增加了50％。将l-NAME（100microM）添加到孵育培养基中完全阻止了GM1诱导的NOx的增加。相反，将切片暴露在GM1（100microM）中60分钟会降低NOx含量，从而揭示GM1的双相效应。我们的结果表明，NO在GM1诱导的血管舒张中起重要作用。

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