摘要:目的 探讨细菌感染性疾病患者白细胞体积(V)、传导性(C)、激光散射性(S)的变化及其临床意义.方法 利用Beckman-Coulter LH750全自动血液分析仪的体积、高频传导、激光散射(VCS)技术测定55例细菌感染性疾病患者以及64例健康人的中性粒细胞(NE)、淋巴细胞(LY)及单核细胞(MO)的体积、核浆比例、胞浆颗粒特性以及它们各自的平均分布宽度(SD).结果 与健康对照组比较,感染组中的NEV、NEV-SD、NEC、NEC-SD、LYV、LYV-SD、LYC-SD、LYS、MOV、MOV-SD、MOC均增高(P<0.05),而NES则减少(P<0.05).在感染组以WBC 10×109/L为界分成的2组中,除NEV、NEV-SD在WBC>10×109/L组中较WBC≤10×109/L组增高外(P<0.05),其他改变的差异均无统计学意义(P>0.05).结论 细菌感染可使外周血白细胞(包括WBC不升高的情况)的VCS参数发生相应改变,检测其VCS参数可作为了解细菌感染程度的辅助指标之一.%Objective To investigate the change and the clinical significance of volume (V), conductibility (C), reflecting karyo-plasmic ratio,and laser light scattering (S), reflecting cytoplasmic granules, of white blood cells (WBC) in patients with bacterial infections. Methods Automatic blood cell analyzer, Beckman-Coulter LH750, with VCS parameters, was used to analyze the volume, karyoplasmic ratio,cytoplasmic granules and related standard deviation (SD) parameters of neutrophils (NE), lymphocytes (LY) and monocytes (MO) of 55 cases of patients with bactereial infections and 64 cases of healthy controls. The volume,conductibility, laser light scattering and related standard deviation of cells, mentioned above, were abbreviated as NEV, NEV-SD, NEC,NEC-SD, NES, NES-SD, LYV, LYV-SD, LYC, LYC-SD, LYS, LYX-SD, MOV, MOV-SD, MOC, MOC-SD, MOS and MOS-SD. Results Compared with the healthy controls, the parameters of NEV, NEV-SD, NEC, NEC-SD, LYV, LYV-SD, LYC-SD, LYS,MOV, MOV-SD and MOC of patients group increased significantly (P<0.05), but NES decreased significantly (P<0.05), whereas the changes of rest parameters were insignificant (P>0.05). Patients group was then divided into 2 subgroups,according to the result of WBC count. Compared with subgroup 1 (WBC count ≤ 10 × 109/L), the NEV and NEV-SD of subgroup 2 (WBC count >10 × 109/L) decreased significantly (P<0.05), and the changes of the other parameters were not significant (P>0.05). Conclusion In patients with bacterial infections, the VCS parameters of WBC change correapondingly with the diseases. The VCS parameters of WBC could be used to identify the severity of bacterial infections.