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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Diacylglyceride lipase activity in rod outer segments depends on the illumination state of the retina.
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Diacylglyceride lipase activity in rod outer segments depends on the illumination state of the retina.

机译：杆外部段中的二酰基甘油酯脂肪酶活性取决于视网膜的照明状态。

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摘要

We have demonstrated that the competition between phosphatidic acid (PA) and lysophosphatidic acid (LPA), sphingosine 1-phosphate (S1P) and ceramide 1-phosphate (C1P) for lipid phosphate phosphatases (LPP) generates different levels of diacylglycerol (DAG) depending on the illumination state of the retina. The aim of the present research was to determine the diacylglyceride lipase (DAGL) activity in purified rod outer segments (ROS) obtained from dark-adapted retinas (DROS) or light-adapted retinas (BLROS) as well as in ROS membrane preparations depleted of soluble and peripheral proteins. [2-(3)H]monoacylglycerol (MAG), the product of DAGL, was evaluated from [2-(3)H]DAG generated by LPP action on [2-(3)H]PA in the presence of either LPA, S1P or C1P. MAG production was inhibited by 55% in BLROS and by 25% when the enzymatic assay was carried out in ROS obtained from dark-adapted retinas and incubated under room light (LROS). The most important events occurred in DROS where co-incubation of [2-(3)H]PA with LPA, S1P or C1P diminished MAG production. A higher level of DAGL activity was observed in LROS than in BLROS, though this difference was not apparent in the presence of LPA, S1P or C1P. DAGL activity in depleted DROS was diminished with respect to that in entire DROS. LPA, S1P and C1P produced a similar decrease in MAG production in depleted DROS whereas only C1P significantly diminished MAG generation in depleted BLROS. Sphingosine and ceramide inhibited MAG production in entire DROS and stimulated its generation in BLROS. Sphingosine and ceramide stimulated MAG generation in both depleted DROS and BLROS. Under our experimental conditions the degree of MAG production depended on the illumination state of the retina. We therefore suggest that proteins related to phototransduction phenomena are involved in the effects observed in the presence of S1P/sphingosine or C1P/ceramide.
机译：我们已经证明，磷脂酰磷酸酶（LPP）的磷脂酸（PA）与溶血磷脂酸（LPA），鞘氨醇1-磷酸（S1P）和神经酰胺1-磷酸（C1P）之间的竞争会产生不同水平的二酰基甘油（DAG），具体取决于视网膜的照明状态本研究的目的是确定从暗适应性视网膜（DROS）或轻适应性视网膜（BLROS）以及从中去除的ROS膜制剂中获得的纯化杆外段（ROS）中的二酰基甘油酯脂肪酶（DAGL）活性。可溶性和外周蛋白。在存在LPA的情况下，由LPP对[2-（3）H] PA的作用产生的[2-（3）H] DAG评估了DAGL的产物[2-（3）H]单酰基甘油（MAG） ，S1P或C1P。当在从黑暗适应的视网膜获得并在室温下孵育的ROS中进行酶促测定时，在BLROS中MAG的产生被抑制了55％，在酶促测定中被抑制了25％。最重要的事件发生在DROS中，其中[2-（3）H] PA与LPA，S1P或C1P共同孵育会降低MAG的产生。尽管在LPA，S1P或C1P存在下这种差异并不明显，但在LROS中观察到的DAGL活性水平高于BLROS。相对于整个DROS，耗尽的DROS中的DAGL活性降低。 LPA，S1P和C1P在枯竭的DROS中产生了类似的MAG产生减少，而只有C1P显着减少了枯竭的BLROS中的MAG产生。鞘氨醇和神经酰胺抑制整个DROS中MAG的产生，并刺激其在BLROS中的生成。鞘氨醇和神经酰胺可在耗尽的DROS和BLROS中刺激MAG的产生。在我们的实验条件下，MAG产生的程度取决于视网膜的照明状态。因此，我们建议与光转导现象有关的蛋白质参与在S1P /鞘氨醇或C1P /神经酰胺存在下观察到的效应。

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