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首页> 外文期刊>International journal of toxicology >Studies of the toxicological potential of tripeptides (L-valyl-L-prolyl-L-proline and L-isoleucyl-L-prolyl-L-proline): VII. Micronucleus test of tripeptides-containing casein hydrolysate and Lactobacillus helveticus-fermented milk powders in rats and
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Studies of the toxicological potential of tripeptides (L-valyl-L-prolyl-L-proline and L-isoleucyl-L-prolyl-L-proline): VII. Micronucleus test of tripeptides-containing casein hydrolysate and Lactobacillus helveticus-fermented milk powders in rats and

机译：三肽（L-戊基-L-脯氨酰-L-脯氨酸和L-异亮氨酰-L-脯氨酰-L-脯氨酸）的毒理学潜力研究：VII。含三肽酪蛋白水解物和瑞士乳杆菌发酵奶粉的微核试验

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The objective of these in vivo experiments was to assess the mutagenic potential of tripeptides as reflected by the ability of the test compound to induce the formation of micronuclei in mouse polychromatic erythrocytes. The test agents used in these experiments were (1) powdered Aspergillus oryzae protease casein hydrolysate (CH) and (2) powdered Lactobacillus helveticus-fermented milk (FM). Both test agents contain two tripeptides, L-valyl-L-prolyl-L-proline (VPP) and L-isoleucyl-L-prolyl-L-proline (IPP). Male Sprague-Dawley rats (five per group) were administered doses of 0, 500, 1000, or 2000 mg (0, 3, 6, or 12 mg VPP plus IPP)/kg body weight (BW)/day CH by oral gavage for 2 days. Male CD-1 mice (six per group) received a single oral gavage dose of 0, 500, 1000, or 2000 mg (0, 0.8, 1.6 or 3.3 mg VPP plus IPP)/kg BW of FM. Positive-control agents were cyclophosphamide (10 mg/kg, intraperitoneal [i.p.]) in rats and mitocycin C (2 mg/kg, i.p.) in mice. Twenty-four hours after the second dose of CH, or the dose of cyclophosphamide to rats, or FM or mitocycin C to mice, bone marrow cells were fixed and examined for the presence of polychromatic erythrocytes (PCEs) and the presence or absence of micronucleated PCEs (MNPCEs). Administration of CH to rats and FM to mice produced neither changes in body weights nor signs of systemic toxicity. Similarly, neither CH nor FM caused statistically significant variations in the incidences of either PCEs or MNPCEs. Both positive-control agents caused unequivocal increases in the incidence of MNPCEs and cyclophosphamide significantly reduced the percent of rat erythrocytes appearing as PCEs. The results of these micronucleus assays conducted with either powdered CH or FM in rats and mice, respectively, show that neither form of the tripeptides possesses the potential to induce micronuclei formation in these rodent species.
机译：这些体内实验的目的是评估由测试化合物诱导小鼠多色红细胞中微核形成的能力所反映的三肽诱变潜力。这些实验中使用的测试剂是（1）米曲霉蛋白酶酪蛋白水解粉（CH）和（2）瑞士乳杆菌发酵奶粉（FM）。两种测试剂均包含两个三肽：L-戊基-L-脯氨酰-L-脯氨酸（VPP）和L-异亮氨酰-L-脯氨酰-L-脯氨酸（IPP）。通过口服管饲法对雄性Sprague-Dawley大鼠（每组五只）给予0、500、1000或2000 mg（0、3、6或12 mg VPP加上IPP）/ kg体重（BW）/天CH的剂量2天。雄性CD-1小鼠（每组六只）接受0、500、1000或2000 mg（0、0.8、1.6或3.3 mg VPP加上IPP）/ kg BW FM的单次口服管饲剂量。阳性对照剂是大鼠中的环磷酰胺（10 mg / kg，腹膜内[i.p.]）和小鼠中的丝裂霉素C（2 mg / kg，i.p。）。第二次服用CH或给大鼠服用环磷酰胺或给小鼠服用FM或丝裂霉素C后二十四小时，固定骨髓细胞并检查是否存在多色红细胞（PCE）和是否存在微核PCE（MNPCE）。对大鼠施用CH和对小鼠施用FM均不会产生体重变化或全身毒性的迹象。同样，CH和FM均未引起PCE或MNPCE发生率的统计学显着变化。两种阳性对照剂均引起MNPCE发生率的明确增加，并且环磷酰胺显着降低了以PCE形式出现的大鼠红细胞的百分比。分别在大鼠和小鼠体内用粉末状CH或FM进行的这些微核分析的结果表明，三肽的两种形式均不具有在这些啮齿动物物种中诱导微核形成的潜力。

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