摘要：
为研究不同月份配种奶山羊妊娠期外周血中生殖激素与生长激素的变化规律,随机选择8月、10月、12月、翌年2月妊娠的关中奶山羊各10只,分别于配种后20 d、75 d、130 d及分娩时采集其静脉血并分离血清,采用ELISA试剂盒检测血清中雌激素(E2)、孕酮(P4)、促卵泡生成素(FSH)、促黄体素(LH)、催乳素(PRL)和生长激素(GH)水平变化规律.结果表明,不同季节怀孕奶山羊在整个妊娠期间,同种激素的变化趋势一致,随着妊娠时间的延长,外周血中E2、P4、PRL和GH含量逐渐升高,FSH和LH含量递减,分娩前E2和P4含量下降,LH回升,FSH仍然下降,PRL和GH继续上升.不同月份妊娠期间外周血中E2、P4、P4、FSH、LH、PRL、GH的均值变化范围分别为17.45～52.90 ng/L,1899.39～6305.63 pmol/L,4.45～6.99 IU/L,142.33～207.09 pg/L,367.37～626.88 ng/L,15.55～28.71 μg/L,奶山羊妊娠月份的不同对其激素水平有一定影响,但影响不大.说明奶山羊在不同月份配种妊娠后对其生殖内分泌无显著影响.%To understand the changes of reproductive hormone and growth hormone of pregnant dairy goats mating in different months,ten dairy goats from central Shaanxi plain pregnant in August,October,December,and next February were randomly selected,and their venous blood was taken after mating 20 d,70 d,130 d and at delivery.Then the blood was centrifuged to take serum,the levels of Estrogen (E2),Progesterone(P4),Follicle-stimulating hormone(FSH),Luteinizing hormone(LH),Prolactin (PRL) and growth hormone (GH) in serum were measured by ELISA kit.The results showed that the changes of the same hormones of the pregnant dairy goats in different seasons were consistent during the whole pregnant period.With the extension of pregnancy,the E2,P4,PRL and GH contents of the serum gradually increased,while the FSH and LH content gradually declined;the E2,P4 content of the serum decreased,LH increased and FSH did not change significantly before delivery.The PRL and GH level of the serum gradually increased during the whole pregnancy period.The range of E2,P4,FSH,PRL and GH changes in peripheral blood were 17.45～52.90 ng/L,1899.39～6305.63 pmoL/L,4.45～6.99 IU/L,142.33～ 207.09 pg/L,367.37～626.88 ng/L,15.55～28.71 tμg/L respectively.Pregnancy in different months had little influence on their hormone levels,which indicated that dairy goats mating in different months do not have significant influence on reproductive endocrinology.
