CD

摘要： 模拟Pb、Cd污染土壤,加入自制的固化剂,进行了毒性浸出试验。试验结果表明,加入质量分数为4%的固化剂后,对于铅为2500 mg/kg,镉为50 mg/kg污染土壤,铅的浸出质量浓度是4.47 mg/L,镉的浸出质量浓度是1.05 mg/L,满足固体废物浸出液中危害成分浓度限值要求。浸出液中的Pb、Cd浸出浓度比模拟污染土样品分别降低85.6%,53.1%,说明Pb、Cd污染土壤添加固化剂做固化稳定处理,是一种较好的方法。

摘要： 以松枝为原料,采用350°C限氧热裂解法制备生物炭,利用元素分析、SEM-EDS、BET-N_(2)、FT-IR、Boehm滴定等分析方法对生物炭进行表征。结果表明:松枝生物炭为多孔结构、平均孔径为8.10nm、比表面积为1.74m^(2)·g^(-1),表面含有—C=O、—OH等含氧官能团。通过盆栽实验,研究松枝生物炭对土壤Cd形态转化及绿豆植株对Cd富集吸收的影响。结果表明:生物炭可提高土壤pH和有机质含量,同时降低土壤弱酸提取态Cd的含量,促进Cd由活性高的弱酸提取态向活性低的残渣态转化,显著降低Cd的生物有效性;施用生物炭可提高绿豆生物量,生物炭5%处理效果最佳,最大提高13.47%;绿豆地上部、地下部Cd含量均显著降低,生物炭5%处理降幅最大,分别为81.86%和43.86%。

摘要： 东南景天(Sedum alfredii Hance)是一种典型的Zn、Cd、Pb的超积累植物,也是一种极佳植物修复材料.本文采用盆栽实验方法,研究东南景天组培苗在几种不同Zn、Cd污染土壤上的生长情况,及其对土壤Zn、Cd的去除效果.预期研究结果可为今后利用东南景天组培苗治理Zn、Cd污染提供一些理论依据.取得主要研究结果:1)东南景天组培苗完整地保留了扦插苗对于重金属的耐受性,并且对于高质量分数的Zn、Cd的环境适应性更强.在3#污染土壤(w(Zn)约2000 mg·kg^(−1))中,组培苗地上部分生物量是对照组的2.3倍;在4#污染土壤(w(Cd)约1344 mg·kg^(−1))中,组培苗地上部分生物量相比于对照组的生物量增加了16.4%.2)东南景天组培苗对复合污染土壤中的Zn、Cd有很强的吸收积累能力.在1#和2#复合污染土壤中,组培苗对Zn的富集系数分别达到9.69和11.09,对Cd的富集系数分别达到12.67和85.36.3)复合污染土壤中的Zn、Cd会发生一定的交互作用,从而影响东南景天组培苗对Zn、Cd的吸收和富集.土壤中低质量分数的Cd会促进植物对Zn的吸收,但Zn却会抑制植物对Cd的吸收富集.此外,生长在复合污染土壤中的东南景天组培苗体内Zn、Cd重金属的分布也会受到影响.

摘要： 为了降低重金属污染土壤的风险,需要研究有效的修复技术.采用可生物降解螯合剂谷氨酸N,N-二乙酸(GLDA)为淋洗剂,通过开展土柱淋洗实验,考察了GLDA对Cd和Zn的修复效果和对土壤理化性质的影响.研究结果表明:GLDA对Cd、Zn的淋出率(61.7%、22.3%)与乙二胺四乙酸(EDTA)的相近,酸可提取态和可还原态的Cd和Zn显著减少,Cd、Zn迁移性和环境风险有效降低.FeS沉淀淋洗液中的Cd和Zn,可以实现GLDA回收利用,再生与新鲜的GLDA对Cd、Zn具有相近的提取能力.GLDA淋洗后采用水冲洗土壤,可以减少GLDA的使用量,也会降低GLDA在土壤中的残留.GLDA淋洗对土壤pH、速效磷、速效钾均有不同程度的改变,并显著增加土壤细颗粒的比例,对土壤结构和质地会造成一定的影响.

摘要： 为探究柚皮生物炭(PC)的理化性质及对Cd的吸附效应,以柚皮为原材料在300,450和600°C绝氧环境下制备柚皮生物炭(分别记为PC_(t1),PC_(t2)和PC_(t3))。通过分析PC理化性质、PC对水环境中和土壤环境中Cd吸附效果以及PC_(t3)对土壤Cd吸附机理,综合研究柚皮生物炭对Cd的吸附效应。结果表明:随着热解温度的增加,PC产率下降,灰分含量和pH值升高。元素含量分析表明,随热解温度增加,C元素占比增加,H和O元素占比则减少,PC的芳香性增大,亲水性和极性减小。SEM分析表明,PC与柚皮吸附剂PS相比,拥有更密集的孔隙结构和表面褶皱。FTIR分析表明,通过热解,PC芳香结构逐渐完善,其表面的羟基、羧基、酯基等含氧官能团参与对Cd的吸附,PC_(t3)对水中Cd的吸附效率达到99.51%。PC加入土壤中培养后,土壤pH值升高,土壤有效态Cd含量下降,PC_(t3)-5使土壤中有效态Cd最高减少15.42%。等温吸附试验和吸附动力学试验表明,PC_(t3)对土壤Cd的吸附能力用Freundlich等温吸附方程和准二级动力学方程拟合最好,PC_(t3)对土壤Cd的吸附以化学作用为主,且为多层异构表面的吸附模式。因此,柚皮生物炭作为一种对土壤Cd的廉价高效吸附剂,具有广泛的应用前景。

摘要： 这张CD十分可疑(如下图)!巴赫,是我熟知的。身为“音乐的父亲”,巴赫不仅作品数量巨大、享誉盛名,也是始于那个年代,音乐家逐渐地超越了贵族庄园里厨师、花匠等服务人员的地位而成为艺术家。我学过巴赫的小提琴协奏曲和组曲PARTITA--后者很不容易拉的,印象深刻;唱片公司TELARC,我知道,从八十年开始,就开始收集他们的CD和LP。

摘要： 在华南西部的右江河谷地区按照10 km×3 km的网度采集71个浅层土壤样品,分析测试样品中Cd和Pb的含量,应用累积频率法和分形分析方法分析计算其环境地球化学基线值,以此为评价标准对本地区浅层土壤的Cd、Pb污染进行评价。结果表明:2种分析计算方法所得到的环境地球化学基线值相差不大,其中Cd的基线上限相差10.3%,基线值相差9.4%,Pb的基线上限相差3.6%,基线值相差6.7%,说明2种方法的计算结果都合理,本文取2种方法的算术平均值作为其环境地球化学基线值,即Pb为25.44 mg/kg,Cd为0.304 mg/kg。Cd的单因子污染评价结果显示轻度污染占67.61%、中度污染占23.94%、重度污染占5.63%,Pb的单因子污染评价显示轻度污染占53.52%、中度污染占25.35%、重度污染占11.27%;内梅罗综合污染评价显示轻度污染占52.11%、中度污染占33.88%、重度污染占8.45%,显示出本区以轻度污染为主,中度污染次之,少数地区达到了重度污染,且中、重度污染区主要分布在研究区东南部和西北部,在中西部地区有一个中度污染点。大多数样品Cd和Pb的地累积指数小于1,说明本区Cd和Pb的累积效应不很明显,但在研究区的东南部Cd和Pb的地累积指数以及中北部Pb的地累积指数达到了中度污染水平。这些均说明研究区以东铝土资源开发和研究区中部火电厂的生产活动对该区Cd、Pb污染有较大的影响。

摘要： 通过对贵州岩溶区19条风化剖面Cd分布特征的研究,初步揭示了贫Cd碳酸盐岩发育的土壤中Cd的富集机制及Cd含量的约束因素。①贫Cd基岩发育的土壤同样可以导致Cd的明显富集,甚至超常富集,且含量峰值通常位于土壤层底部(T1)。②Cd在基岩中通常优先赋存在酸不溶相,另一方面,由于基岩酸不溶物含量一般极低,酸溶相的Cd占全岩Cd的比例仍处于绝对优势。在富Cd的基岩酸不溶物基础上,叠加了来自酸溶相中Cd的贡献,是岩溶区土壤Cd普遍富集的原因。③土壤中Cd的含量与其在基岩或基岩酸不溶物中的含量均不相关。T1中Cd的含量受制于Cd在基岩酸不溶物中的含量、基岩酸溶相Cd占全岩中Cd的质量百分数、T1中Cd的亏损率的共同约束。有利于Cd在T1中超常富集的最佳条件是:Cd在基岩酸不溶物中含量高、基岩酸溶相Cd占全岩中Cd的比例大、T1中Cd的亏损率小。另外,对于由基岩酸不溶物残余累积形成的土壤层,T1为其发育起点,Cd在T1中含量高,通常在土壤层中也高,反映出一般风化剖面的发育特征。研究结果可以深化对岩溶环境Cd地球化学行为的认识,为区域上基于地质成因开展Cd污染风险评价及建立其清洁水平提供参考。

摘要： 研究通过盆栽实验,揭示海泡石对Cd污染土壤的钝化修复效果,研究土壤pH值、白菜生物量、Cd有效态含量和品质安全受海泡石浓度的影响,进而推广应用于大面积农田Cd污染土壤的处理。通过研究发现,海泡石在3个含量梯度的(1 mg·kg^(-1)、2 mg·kg^(-1)和5 mg·kg^(-1))污染土壤中,Cd对白菜的胁迫效应有所缓解,作物的生物量具有一定程度的增加,且抑制了白菜对Cd的吸收,白菜体内的镉含量下降了35.89%~47.42%,添加海泡石后,土壤pH的变化不大,总体维持在7.60~7.85,土壤脲酶活性具有显著增加,与未添加海泡石的土壤中脲酶活性增加了77.16%~268.1%。研究证明施加海泡石可以有效治理土壤重金属污染问题。

摘要： 电动修复是一门具有广阔发展前景的土壤修复技术,但其涉及相关机理复杂,目前该技术主要处于室内试验阶段。在电动修复过程中,预测重金属的迁移效果是指导实际应用的重要基石。本文通过质量守恒定律,推导出重金属电动修复的数学模型,使用数值模拟计算模型并设计实验进行验证,最后对电动修复Cd污染土壤的分布趋势进行分析。结果表明在一定条件下,使用数值模拟能够很好地描述电动修复土壤中Cd迁移趋势。

摘要： A greenhouse pot experiment was conducted to evaluate the effect of fungus, of citric acid, or of acombination of both, in the phytoremediation of a heavy metal contaminated soil, affected by Cd and Pb. Sixenzyme activities (namely dehydrogenase, phosphatase, urease, catalase, sucrase and amylase) in soil weredetermined in this study. Detrended Canonical Correspondence Analysis (DCCA) was taken to express therelationship between all remediation methods and soil enzyme activities in the ordination diagram to assessthe remediation effect in soil health degree. Results showed that soil enzyme activities were graduallyinhibited with increasing heavy metal concentration. In the samples without any remediation, single Pbpollution showed smaller influence on inhibition of soil enzyme activity than single Cd pollution, while Cdand Pb combined pollution showed similar changing tendency with single Cd pollution. As shown in theordination graph by DCCA, All soil enzymes variables showed big change at first axis. In a certain extent, Cdand Pb complex variables could reflect the soil enzymes activities change law at first axis due to their biggerprojections at first axis. The ordination diagrams also expressed the relationship between all remediationmethod variables and soil enzyme activity variables by their distance and position. Most of remediationmethods had positive effects on enhancing soil enzyme activities, except on sucrase activity. The soil enzymeactivities of hyperaccumulator treated with fungus were similar to that of treated with citric acid. Allremediation methods had significant positive effects on enhancing dehydrogenase, urease and catalaseactivities, whereas phosphatase and amylase activities were reduced at low heavy metal concentration andincreased at high concentration as a result of remediation in soil. All remediation methods showed negativeeffects on sucrase activity.

摘要： A greenhouse pot experiment was conducted to evaluate the effect of fungus, of citric acid, or of acombination of both, in the phytoremediation of a heavy metal contaminated soil, affected by Cd and Pb. Sixenzyme activities (namely dehydrogenase, phosphatase, urease, catalase, sucrase and amylase) in soil weredetermined in this study. Detrended Canonical Correspondence Analysis (DCCA) was taken to express therelationship between all remediation methods and soil enzyme activities in the ordination diagram to assessthe remediation effect in soil health degree. Results showed that soil enzyme activities were graduallyinhibited with increasing heavy metal concentration. In the samples without any remediation, single Pbpollution showed smaller influence on inhibition of soil enzyme activity than single Cd pollution, while Cdand Pb combined pollution showed similar changing tendency with single Cd pollution. As shown in theordination graph by DCCA, All soil enzymes variables showed big change at first axis. In a certain extent, Cdand Pb complex variables could reflect the soil enzymes activities change law at first axis due to their biggerprojections at first axis. The ordination diagrams also expressed the relationship between all remediationmethod variables and soil enzyme activity variables by their distance and position. Most of remediationmethods had positive effects on enhancing soil enzyme activities, except on sucrase activity. The soil enzymeactivities of hyperaccumulator treated with fungus were similar to that of treated with citric acid. Allremediation methods had significant positive effects on enhancing dehydrogenase, urease and catalaseactivities, whereas phosphatase and amylase activities were reduced at low heavy metal concentration andincreased at high concentration as a result of remediation in soil. All remediation methods showed negativeeffects on sucrase activity.

摘要： A greenhouse pot experiment was conducted to evaluate the effect of fungus, of citric acid, or of acombination of both, in the phytoremediation of a heavy metal contaminated soil, affected by Cd and Pb. Sixenzyme activities (namely dehydrogenase, phosphatase, urease, catalase, sucrase and amylase) in soil weredetermined in this study. Detrended Canonical Correspondence Analysis (DCCA) was taken to express therelationship between all remediation methods and soil enzyme activities in the ordination diagram to assessthe remediation effect in soil health degree. Results showed that soil enzyme activities were graduallyinhibited with increasing heavy metal concentration. In the samples without any remediation, single Pbpollution showed smaller influence on inhibition of soil enzyme activity than single Cd pollution, while Cdand Pb combined pollution showed similar changing tendency with single Cd pollution. As shown in theordination graph by DCCA, All soil enzymes variables showed big change at first axis. In a certain extent, Cdand Pb complex variables could reflect the soil enzymes activities change law at first axis due to their biggerprojections at first axis. The ordination diagrams also expressed the relationship between all remediationmethod variables and soil enzyme activity variables by their distance and position. Most of remediationmethods had positive effects on enhancing soil enzyme activities, except on sucrase activity. The soil enzymeactivities of hyperaccumulator treated with fungus were similar to that of treated with citric acid. Allremediation methods had significant positive effects on enhancing dehydrogenase, urease and catalaseactivities, whereas phosphatase and amylase activities were reduced at low heavy metal concentration andincreased at high concentration as a result of remediation in soil. All remediation methods showed negativeeffects on sucrase activity.

摘要： A greenhouse pot experiment was conducted to evaluate the effect of fungus, of citric acid, or of acombination of both, in the phytoremediation of a heavy metal contaminated soil, affected by Cd and Pb. Sixenzyme activities (namely dehydrogenase, phosphatase, urease, catalase, sucrase and amylase) in soil weredetermined in this study. Detrended Canonical Correspondence Analysis (DCCA) was taken to express therelationship between all remediation methods and soil enzyme activities in the ordination diagram to assessthe remediation effect in soil health degree. Results showed that soil enzyme activities were graduallyinhibited with increasing heavy metal concentration. In the samples without any remediation, single Pbpollution showed smaller influence on inhibition of soil enzyme activity than single Cd pollution, while Cdand Pb combined pollution showed similar changing tendency with single Cd pollution. As shown in theordination graph by DCCA, All soil enzymes variables showed big change at first axis. In a certain extent, Cdand Pb complex variables could reflect the soil enzymes activities change law at first axis due to their biggerprojections at first axis. The ordination diagrams also expressed the relationship between all remediationmethod variables and soil enzyme activity variables by their distance and position. Most of remediationmethods had positive effects on enhancing soil enzyme activities, except on sucrase activity. The soil enzymeactivities of hyperaccumulator treated with fungus were similar to that of treated with citric acid. Allremediation methods had significant positive effects on enhancing dehydrogenase, urease and catalaseactivities, whereas phosphatase and amylase activities were reduced at low heavy metal concentration andincreased at high concentration as a result of remediation in soil. All remediation methods showed negativeeffects on sucrase activity.

摘要： A greenhouse pot experiment was conducted to evaluate the effect of fungus, of citric acid, or of acombination of both, in the phytoremediation of a heavy metal contaminated soil, affected by Cd and Pb. Sixenzyme activities (namely dehydrogenase, phosphatase, urease, catalase, sucrase and amylase) in soil weredetermined in this study. Detrended Canonical Correspondence Analysis (DCCA) was taken to express therelationship between all remediation methods and soil enzyme activities in the ordination diagram to assessthe remediation effect in soil health degree. Results showed that soil enzyme activities were graduallyinhibited with increasing heavy metal concentration. In the samples without any remediation, single Pbpollution showed smaller influence on inhibition of soil enzyme activity than single Cd pollution, while Cdand Pb combined pollution showed similar changing tendency with single Cd pollution. As shown in theordination graph by DCCA, All soil enzymes variables showed big change at first axis. In a certain extent, Cdand Pb complex variables could reflect the soil enzymes activities change law at first axis due to their biggerprojections at first axis. The ordination diagrams also expressed the relationship between all remediationmethod variables and soil enzyme activity variables by their distance and position. Most of remediationmethods had positive effects on enhancing soil enzyme activities, except on sucrase activity. The soil enzymeactivities of hyperaccumulator treated with fungus were similar to that of treated with citric acid. Allremediation methods had significant positive effects on enhancing dehydrogenase, urease and catalaseactivities, whereas phosphatase and amylase activities were reduced at low heavy metal concentration andincreased at high concentration as a result of remediation in soil. All remediation methods showed negativeeffects on sucrase activity.

摘要： A greenhouse pot experiment was conducted to evaluate the effect of fungus, of citric acid, or of acombination of both, in the phytoremediation of a heavy metal contaminated soil, affected by Cd and Pb. Sixenzyme activities (namely dehydrogenase, phosphatase, urease, catalase, sucrase and amylase) in soil weredetermined in this study. Detrended Canonical Correspondence Analysis (DCCA) was taken to express therelationship between all remediation methods and soil enzyme activities in the ordination diagram to assessthe remediation effect in soil health degree. Results showed that soil enzyme activities were graduallyinhibited with increasing heavy metal concentration. In the samples without any remediation, single Pbpollution showed smaller influence on inhibition of soil enzyme activity than single Cd pollution, while Cdand Pb combined pollution showed similar changing tendency with single Cd pollution. As shown in theordination graph by DCCA, All soil enzymes variables showed big change at first axis. In a certain extent, Cdand Pb complex variables could reflect the soil enzymes activities change law at first axis due to their biggerprojections at first axis. The ordination diagrams also expressed the relationship between all remediationmethod variables and soil enzyme activity variables by their distance and position. Most of remediationmethods had positive effects on enhancing soil enzyme activities, except on sucrase activity. The soil enzymeactivities of hyperaccumulator treated with fungus were similar to that of treated with citric acid. Allremediation methods had significant positive effects on enhancing dehydrogenase, urease and catalaseactivities, whereas phosphatase and amylase activities were reduced at low heavy metal concentration andincreased at high concentration as a result of remediation in soil. All remediation methods showed negativeeffects on sucrase activity.

摘要： A greenhouse pot experiment was conducted to evaluate the effect of fungus, of citric acid, or of acombination of both, in the phytoremediation of a heavy metal contaminated soil, affected by Cd and Pb. Sixenzyme activities (namely dehydrogenase, phosphatase, urease, catalase, sucrase and amylase) in soil weredetermined in this study. Detrended Canonical Correspondence Analysis (DCCA) was taken to express therelationship between all remediation methods and soil enzyme activities in the ordination diagram to assessthe remediation effect in soil health degree. Results showed that soil enzyme activities were graduallyinhibited with increasing heavy metal concentration. In the samples without any remediation, single Pbpollution showed smaller influence on inhibition of soil enzyme activity than single Cd pollution, while Cdand Pb combined pollution showed similar changing tendency with single Cd pollution. As shown in theordination graph by DCCA, All soil enzymes variables showed big change at first axis. In a certain extent, Cdand Pb complex variables could reflect the soil enzymes activities change law at first axis due to their biggerprojections at first axis. The ordination diagrams also expressed the relationship between all remediationmethod variables and soil enzyme activity variables by their distance and position. Most of remediationmethods had positive effects on enhancing soil enzyme activities, except on sucrase activity. The soil enzymeactivities of hyperaccumulator treated with fungus were similar to that of treated with citric acid. Allremediation methods had significant positive effects on enhancing dehydrogenase, urease and catalaseactivities, whereas phosphatase and amylase activities were reduced at low heavy metal concentration andincreased at high concentration as a result of remediation in soil. All remediation methods showed negativeeffects on sucrase activity.

摘要： 通过盆栽模拟试验研究了甘蔗长期受重金属镉(Cd)胁迫的生理响应。结果表明甘蔗生物量、茎径和茎节长随着土壤中Cd浓度的增加而降低，Cd浓度越高，抑制效应越明显;叶片细胞质膜透性与土壤中CA浓度呈显著正相关性(P<0.05)，相关系数为0.867：叶片中叶绿素a、叶绿素b、过氧化氢酶(CAT)活性及蔗茎中可溶性糖含量与土壤中Cd浓度呈显著负相关性(P<0.05)，相关系数分别为0.901、0.940、0.841和0.795：甘蔗能在Cd浓度为1000mg/kg的土壤中存活。研究结果提示，甘蔗对Cd胁迫有较好的耐性，具有植物修复Cd污染土壤的潜力。

摘要： 通过盆栽模拟试验研究了甘蔗长期受重金属镉(Cd)胁迫的生理响应。结果表明甘蔗生物量、茎径和茎节长随着土壤中Cd浓度的增加而降低，Cd浓度越高，抑制效应越明显;叶片细胞质膜透性与土壤中CA浓度呈显著正相关性(P<0.05)，相关系数为0.867：叶片中叶绿素a、叶绿素b、过氧化氢酶(CAT)活性及蔗茎中可溶性糖含量与土壤中Cd浓度呈显著负相关性(P<0.05)，相关系数分别为0.901、0.940、0.841和0.795：甘蔗能在Cd浓度为1000mg/kg的土壤中存活。研究结果提示，甘蔗对Cd胁迫有较好的耐性，具有植物修复Cd污染土壤的潜力。

摘要： 通过盆栽模拟试验研究了甘蔗长期受重金属镉(Cd)胁迫的生理响应。结果表明甘蔗生物量、茎径和茎节长随着土壤中Cd浓度的增加而降低，Cd浓度越高，抑制效应越明显;叶片细胞质膜透性与土壤中CA浓度呈显著正相关性(P<0.05)，相关系数为0.867：叶片中叶绿素a、叶绿素b、过氧化氢酶(CAT)活性及蔗茎中可溶性糖含量与土壤中Cd浓度呈显著负相关性(P<0.05)，相关系数分别为0.901、0.940、0.841和0.795：甘蔗能在Cd浓度为1000mg/kg的土壤中存活。研究结果提示，甘蔗对Cd胁迫有较好的耐性，具有植物修复Cd污染土壤的潜力。

摘要： 本发明涉及与CD3特异性结合并与至少另一种抗原例如CD123特异性结合的抗体样结合蛋白。本发明还涉及与CD123特异性结合并与至少另一种抗原特异性结合的抗体样结合蛋白。本发明进一步涉及抗CD3抗体和抗CD123抗体。本发明还涉及包含本发明抗体样结合蛋白、抗CD3抗体或抗CD123抗体的药物组合物和其治疗癌症的用途。本发明进一步涉及包含编码所述抗体样结合蛋白、抗CD3或抗CD123抗体的序列的分离的核酸、载体和宿主细胞和所述抗CD123抗体作为诊断工具的用途。

摘要： 本发明涉及一种检测CD105、CD144、CD34、KDR、Annexin V和CD63的试剂在制备检测血液中的内皮及内皮祖细胞释放的细胞外囊泡的试剂中的应用，从循环系统中分别分离出EC‑MVs、EPC‑MVs、EC‑EXs和EPC‑EXs。该方法突破了以往用单特异性抗体的方法且提取出来的细胞MVs和EXs比现有技术的特异性和敏感性高。此外，本发明利用纳米微粒追踪分析术(NTS)针对细胞MVs和EXs进行特异性抗体micro‑beads方法结合荧光量子点(Q‑dots)方法进行检测。该方法能快速，准确，客观分析复杂样本例如血液中EC‑MVs,EPC‑MVs,EC‑EXs和EPC‑EXs的水平，有传统分析方法例如流式所不具备的优势。因此，本发明为进一步研究MVs和EXs作为疾病的生物标记物提供了特异和敏感的方法。

摘要： 本发明公开了一种同时高效扩增CD3+CD56+CIK细胞和CD3‑CD56+NK细胞的方法，步骤为：用含自体血浆的无血清培养基调整分离的PBMC浓度加入Anti‑CD16抗体、IL‑2、IL‑15后转入T175培养瓶中培养，后再加入Anti‑CD3抗体、Anti‑CD137抗体，根据细胞的生长情况，每隔2天补充含自体血浆、IL‑2和IL‑15的无血清培养基，控制细胞浓度约1.5×106/ml，培养14～21天后可同时获得大量较高纯度的CD3+CD56+CIK和CD3‑CD56+NK细胞，且总细胞数量能达到临床用于肿瘤过继性细胞免疫治疗所需细胞数量的有效值。本发明简便、有效，细胞杀伤活性高。

摘要： 本发明目的在于提供对各种疾病发挥优异的治疗效果的新型的间充质干细胞及包含该间充质干细胞的新型的药物组合物、以及它们的制备方法。本发明为表达选自由CD201、CD46、CD56、CD147和CD165组成的组中的至少1种细胞表面标记物的间充质干细胞。表达上述特定标记物的间充质干细胞为CD29、CD73、CD90、CD105和CD166阳性，维持了未分化性。

摘要： 本发明涉及抗CD3抗体、抗CD123抗体和与CD3和/或CD123特异性结合的双特异性抗体，具体涉及与CD3特异性结合并与至少另一种抗原例如CD123特异性结合的抗体样结合蛋白。本发明还涉及与CD123特异性结合并与至少另一种抗原特异性结合的抗体样结合蛋白。本发明进一步涉及抗CD3抗体和抗CD123抗体。本发明还涉及包含本发明抗体样结合蛋白、抗CD3抗体或抗CD123抗体的药物组合物和其治疗癌症的用途。本发明进一步涉及包含编码所述抗体样结合蛋白、抗CD3或抗CD123抗体的序列的分离的核酸、载体和宿主细胞和所述抗CD123抗体作为诊断工具的用途。

摘要： 本发明涉及抗CD3抗体、抗CD123抗体和与CD3和/或CD123特异性结合的双特异性抗体，具体涉及与CD3特异性结合并与至少另一种抗原例如CD123特异性结合的抗体样结合蛋白。本发明还涉及与CD123特异性结合并与至少另一种抗原特异性结合的抗体样结合蛋白。本发明进一步涉及抗CD3抗体和抗CD123抗体。本发明还涉及包含本发明抗体样结合蛋白、抗CD3抗体或抗CD123抗体的药物组合物和其治疗癌症的用途。本发明进一步涉及包含编码所述抗体样结合蛋白、抗CD3或抗CD123抗体的序列的分离的核酸、载体和宿主细胞和所述抗CD123抗体作为诊断工具的用途。

摘要： 本发明涉及结合CD3和CD137(4‑1BB)的抗原结合分子，包含所述抗原结合分子的组合物及其使用方法。本发明提供了抗原结合分子，其包含能够结合CD3和CD137(4‑1BB)但不同时结合CD3和CD137的抗体可变区，以及与不同于CD3和CD137的第三抗原结合的可变区。这些抗原结合分子通过与三种不同抗原的结合而表现出由这些抗原结合分子诱导的增强的T细胞依赖性细胞毒活性。

摘要： 本发明涉及与CD3特异性结合并与至少另一种抗原例如CD123特异性结合的抗体样结合蛋白。本发明还涉及与CD123特异性结合并与至少另一种抗原特异性结合的抗体样结合蛋白。本发明进一步涉及抗CD3抗体和抗CD123抗体。本发明还涉及包含本发明抗体样结合蛋白、抗CD3抗体或抗CD123抗体的药物组合物和其治疗癌症的用途。本发明进一步涉及包含编码所述抗体样结合蛋白、抗CD3或抗CD123抗体的序列的分离的核酸、载体和宿主细胞和所述抗CD123抗体作为诊断工具的用途。

摘要： 本发明总体涉及特异性结合至少CD3的抗体、可活化抗体、多特异性抗体和多特异性可活化抗体，以及制备这些抗体、可活化抗体、多特异性抗体和/或多特异性可活化抗体和在各种治疗、诊断和预防适应症中使用这些抗体、可活化抗体、多特异性抗体和/或多特异性可活化抗体的方法。

摘要： 本发明公开了一种同时高效扩增CD3+CD56+CIK细胞和CD3-CD56+NK细胞的方法，步骤为：用含自体血浆的无血清培养基调整分离的PBMC浓度加入Anti-CD16抗体、IL-2、IL-15后转入T175培养瓶中培养，后再加入Anti-CD3抗体、Anti-CD137抗体，根据细胞的生长情况，每隔2天补充含自体血浆、IL-2和IL-15的无血清培养基，控制细胞浓度约1.5×106/ml，培养14～21天后可同时获得大量较高纯度的CD3+CD56+CIK和CD3-CD56+NK细胞，且总细胞数量能达到临床用于肿瘤过继性细胞免疫治疗所需细胞数量的有效值。本发明简便、有效，细胞杀伤活性高。