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首页> 外文期刊>Cell biology international. >Phosphatidylinositol-4-phosphate 5-kinase gamma is associated with cell-cell junction in A431 epithelial cells.
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Phosphatidylinositol-4-phosphate 5-kinase gamma is associated with cell-cell junction in A431 epithelial cells.

机译：磷脂酰肌醇-4-磷酸5-激酶γ与A431上皮细胞中的细胞-细胞连接有关。

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摘要

Cell to cell contact in epithelial cells is crucial for tissue integrity and is maintained by junctional complexes, such as the adherens junction (AJ). Actin polymerization has been shown to be important for AJ formation; however, the molecular mechanisms have yet to be clarified. It has been shown that increased phosphatidylinositol-4,5-bisphosphate (PIP2) induces actin polymerization. It is thus of interest to know more about the production of PIP2 during cell-cell adhesion formation in epithelial cells. The distribution of phosphatidylinositol-4-phosphate 5-kinase gamma635 (PIP5Kgamma635), an isoform of the PIP2 synthesizing enzymes, was examined in epithelial cell line A431. It was found that, in non-contact cells, PIP5Kgamma635 was not concentrated at the plasma membrane. However, in cells that were in contact, PIP5Kgamma635 localized to the intercellular contact sites and colocalized with E-cadherin and beta-catenin, two components of AJ, and with polymerized actin, but did not colocalize with focal adhesion, integrin-mediated cell-substratum complex. Decreasing calcium ion concentration induced both disruption of intercellular adhesion and the dissociation of both PIP5Kgamma635 and actin from the contact site. These results suggest that PIP5K has an important role in actin polymerization in epithelial cell-cell adhesion.
机译：上皮细胞之间的细胞间接触对于组织的完整性至关重要，并通过连接复合物（如粘附连接（AJ））来维持。肌动蛋白聚合已被证明对AJ的形成很重要。然而，其分子机制尚未阐明。已经表明增加的磷脂酰肌醇-4，5-双磷酸酯（PIP2）诱导肌动蛋白聚合。因此，有兴趣了解更多关于上皮细胞中细胞间粘附形成过程中PIP2的产生的信息。在上皮细胞系A431中检查了磷脂酰肌醇-4-磷酸5-激酶γ635（PIP5Kg635）的分布，PIP2合成酶的同工型。发现在非接触细胞中，PIP5Kgamma635没有集中在质膜上。但是，在接触的细胞中，PIP5Kgamma635定位于细胞间接触位点，并与AJ的两个成分E-cadherin和β-catenin以及与聚合的肌动蛋白共定位，但与粘着斑并不整合，整合素介导的细胞基质复合体。钙离子浓度的降低引起细胞间粘附的破坏以及PIP5Kgamma635和肌动蛋白从接触部位的解离。这些结果表明，PIP5K在肌动蛋白聚合中上皮细胞-细胞粘附中具有重要作用。

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