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首页> 外文期刊>Cell biology international. >Transfection of spermatozoa in bivalve molluscs using naked DNA.
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Transfection of spermatozoa in bivalve molluscs using naked DNA.

机译：使用裸露的DNA转染双壳软体动物中的精子。

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摘要

Spermatozoa from the bivalve molluscs Mytilus galloprovincialis, Mytilus chilensis and Chamelea gallina were transfected in vitro using the p-GeneGrip construct, which encodes green fluorescent protein. The efficiency of transfection after brief incubation was assessed by fluorescence and confocal laser microscopy, and was about 58.5-70.01% in the species used. The foreign gene was principally located in the sperm nuclei, as demonstrated by laser confocal serial sections. In some spermatozoa, mitochondria, which are grouped in the base of the nucleus, also appeared to be transfected. Polymerase chain reaction and Southern blot analyses suggested that the foreign DNA had been integrated into the nuclear genome in Mytilus galloprovincialis spermatozoa. This simple method for spermatozoon transfection in molluscs of commercial interest could have biotechnological applications.
机译：使用编码绿色荧光蛋白的p-GeneGrip构建体在体外转染双壳软体动物Mytilus galloprovincialis，智利Mytilus chilensis和Chamelea gallina的精子。短暂孵育后的转染效率通过荧光和共聚焦激光显微镜评估，在所用物种中约为58.5-70.01％。激光共聚焦系列切片显示，外源基因主要位于精子细胞核中。在一些精子中，线粒体也被转染了，这些线粒体位于细胞核的底部。聚合酶链反应和Southern印迹分析表明，外来DNA已被整合入Mytilus galloprovincialis spermatozoa的核基因组中。这种在商业上感兴趣的软体动物中进行精子转染的简单方法可能具有生物技术应用。

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