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首页> 外文期刊>Cell biology international. >Effects of coumarin and 7OH-coumarin on bcl-2 and Bax expression in two human lung cancer cell lines in vitro.
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Effects of coumarin and 7OH-coumarin on bcl-2 and Bax expression in two human lung cancer cell lines in vitro.

机译：香豆素和7OH-香豆素对两种人肺癌细胞体外bcl-2和Bax表达的影响。

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摘要

Coumarin and its derivative 7-hydroxycoumarin (7-OHC) have antitumor and antimetastatic properties. The purpose of this study was to investigate the possible effects of these compounds on expression of the bcl-2 and Bax oncoproteins in two human lung cancer cell lines, A427 and Calu-1. The cells were cultured in vitro for 24 h in RPMI 1640 with 1.5% (v/v) ethanol, 1.0 mM ethanolic coumarin or 1.0 mM ethanolic 7-OHC. Viability was determined in each cell line by an MTT assay. Total protein was extracted from cell lysates and the bcl-2 and Bax oncoproteins were identified. Western blotting showed a decrease in bcl-2 and an increase in Bax in A427 cells cultured with coumarin or 7-OHC. Neither drug changed bcl-2 expression in Calu-1 cells compared to solvent controls, and Bax expression was only slightly increased by coumarin. We conclude that 7-OHC is a more potent inhibitor of cell proliferation than coumarin and has more marked effects on oncoprotein expression. Also, the A427 cell line was more sensitive to the drugs than Calu-1.
机译：香豆素及其衍生物7-羟基香豆素（7-OHC）具有抗肿瘤和抗转移的特性。这项研究的目的是研究这些化合物对两种人类肺癌细胞系A427和Calu-1中bcl-2和Bax癌蛋白表达的可能影响。将细胞在含有1.5％（v / v）乙醇，1.0 mM乙醇香豆素或1.0 mM乙醇7-OHC的RPMI 1640中体外培养24小时。通过MTT测定法确定每种细胞系中的生存力。从细胞裂解物中提取总蛋白，并鉴定bcl-2和Bax癌蛋白。 Western印迹显示，香豆素或7-OHC培养的A427细胞中bcl-2减少，Bax增加。与溶剂对照组相比，这两种药物都没有改变Calu-1细胞中的bcl-2表达，香豆素仅使Bax表达略有增加。我们得出的结论是7-OHC比香豆素是更有效的细胞增殖抑制剂，并且对癌蛋白表达的影响更大。而且，A427细胞系比Calu-1对药物更敏感。

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