Fibrin clots keep non-adhering living cells in place on glass for perfusion or fixation.

Forer A; Pickett Heaps J

首页> 外文期刊>Cell biology international. >Fibrin clots keep non-adhering living cells in place on glass for perfusion or fixation.

【24h】

Fibrin clots keep non-adhering living cells in place on glass for perfusion or fixation.

机译：纤维蛋白凝块将非粘附性活细胞固定在玻璃上以进行灌注或固定。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We describe a method to hold living cells in place that ordinarily do not adhere to glass coverslips. The method, developed for insect spermatocytes but with application to other cell types, consists of embedding cells in a fibrin clot that forms after the enzyme thrombin cleaves the blood protein fibrinogen. The method permits continuous observation of living cells as they are treated with and recover from drug or other treatments: when held in the clot the living cells remain in place and keep their shapes when perfused with drugs that ordinarily cause drastic shape changes, and they remain in place and keep their shapes through lysis/fixation procedures. We describe how to place live cells in a fibrin clot and how subsequently to perfuse them.

机译：我们描述了一种将活细胞固定在通常不粘附在玻璃盖玻片上的方法。该方法是为昆虫的精子细胞开发的，但也适用于其他细胞类型，该方法包括将细胞包埋在血纤维蛋白凝块中，血凝块是在酶凝血酶裂解血蛋白纤维蛋白原后形成的。该方法可在用药物或其他方法处理活细胞并从中恢复时连续观察活细胞：将活细胞保存在血凝块中后，其位置保持不变，并在灌注通常会引起剧烈形状变化的药物时保持其形状，并且它们仍然存在固定并通过裂解/固定程序保持其形状。我们描述了如何将活细胞放置在纤维蛋白凝块中以及随后如何灌注它们。

著录项

来源
《Cell biology international.》 |2005年第9期|共10页
作者
Forer A; Pickett Heaps J;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类细胞生物学;
关键词
Places; livin; g lt; 3gt; Psychological fixation; Glass; 玻璃;

机译：Places;livin;g 3;Psychological fixation;Glass;玻璃;

相似文献

外文文献
中文文献
专利

1. Fibrin clots keep non-adhering living cells in place on glass for perfusion or fixation. [J] . Forer A, Pickett Heaps J Cell biology international. . 2005,第9期

机译：纤维蛋白凝块将非粘附性活细胞固定在玻璃上以进行灌注或固定。
2. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte- and platelet-rich fibrin (L-PRF) clot and membrane [J] . Ehrenfest David M. Dohan, Pinto Nelson R., Pereda Andrea, Platelets . 2018,第2期

机译：离心机特性和离心方案对白细胞和富含白细胞和血小板纤维蛋白（L-PRF）凝块和膜的细胞，生长因子和纤维蛋白结构的影响
3. Bioactivity of the vascular endothelial growth factor trapped in fibrin clots: production of IL-6 and IL-8 in monocytes by fibrin clots. [J] . Tezono K, Sarker KP, Kikuchi H, Haemostasis . 2001,第2期

机译：捕获在纤维蛋白凝块中的血管内皮生长因子的生物活性：纤维蛋白凝块在单核细胞中产生IL-6和IL-8。
4. A synthetic heparin antidote with negligible effect on fibrin(ogen), clotting and clot morphology [C] . Manu Thomas Kalathottukaren, Benjamin FL Lai, Libin Abraham, World biomaterials congress . 2016

机译：合成的肝素解毒剂对血纤蛋白（原），凝血和血块形态的影响可忽略不计
5. The role of different cell types on thrombin generation and fibrin clot formation and structure. [D] . Campbell, Robert A. 2009

机译：不同细胞类型对凝血酶生成和纤维蛋白凝块形成与结构的作用。
6. Role of clot-associated (-derived) thrombin in cell proliferation induced by fibrin clots in vitro [O] . E Gandossi, C Lunven, C N Berry 2000

机译：凝块相关（衍生）凝血酶在纤维蛋白凝块体外诱导细胞增殖中的作用
7. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte- and platelet-rich fibrin (L-PRF) clot and membrane [O] . Dohan Ehrenfest, David M, Pinto, Nelson R, Pereda, Andrea, 2017

机译：离心特性和离心方案对富含白细胞和血小板的纤维蛋白（L-PRF）凝块和膜的细胞，生长因子和纤维蛋白结构的影响

Fibrin clots keep non-adhering living cells in place on glass for perfusion or fixation.

摘要

著录项

相似文献

相关主题

期刊订阅