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首页> 外文期刊>Cell biology international. >A novel alkali-tolerant Yarrowia lipolytica strain for dissecting Na+-coupled phosphate transport systems in yeasts.
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A novel alkali-tolerant Yarrowia lipolytica strain for dissecting Na+-coupled phosphate transport systems in yeasts.

机译:一种新颖的耐碱耶氏酵母解脂耶氏酵母菌株,用于剖析酵母中Na +偶联的磷酸盐转运系统。

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摘要

The newly isolated osmo-, salt- and alkali-tolerant Yarrowia lipolytica yeast strain is remarkable by its capacity to grow at alkaline pH values (pH 9.7), which makes it an excellent model system for studying Na(+)-coupled phosphate transport systems in yeast cells grown at alkaline conditions. In cells Y. lipolytica grown at pH 9.7, phosphate uptake was mediated by several kinetically discrete Na(+)-dependent systems that are specifically activated by Na(+) ions. One of these, a low-affinity transporter, operated at high-phosphate concentrations. The other two, derepressible, high-affinity, high-capacity systems, functioned during phosphate starvation. Both H(+)- and Na(+)-coupled high-affinity phosphate transport systems of Y. lipolytica cells were under the dual control of the prevailing extracellular phosphate concentrations and pH values. The contribution of the Na(+)/P(i)-cotransport systems into the total cellular phosphate uptake activity was progressively increased with increasing pH, reachingits maximum at pH > or = 9.
机译:新分离的耐渗透,耐盐和耐碱的解脂耶氏酵母酵母菌株以在碱性pH值(pH 9.7)下生长的能力而著称,这使其成为研究Na(+)偶联的磷酸盐转运系统的出色模型系统在碱性条件下生长的酵母细胞中在pH 9.7下生长的解脂耶氏酵母细胞中,磷酸盐的吸收是由Na(+)离子特异性激活的几种动力学离散的Na(+)依赖性系统介导的。其中之一,低亲和力转运蛋白,在高磷酸盐浓度下运行。另外两个可抑制的,高亲和力,高容量的系统在磷酸盐饥饿期间起作用。解脂耶氏酵母细胞的H(+)-和Na(+)-偶联的高亲和力磷酸盐转运系统都在主要的细胞外磷酸盐浓度和pH值的双重控制下。 Na(+)/ P(i)-共转运系统对总细胞磷酸盐吸收活性的贡献随着pH的增加而逐渐增加,在pH≥9时达到最大值。

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