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首页> 外文期刊>Cell biology international. >In vitro differentiation of human dental follicle cells with dexamethasone and insulin.
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In vitro differentiation of human dental follicle cells with dexamethasone and insulin.

机译：地塞米松和胰岛素对人牙囊细胞的体外分化。

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The dental follicle is an ectomesenchymally derived connective tissue harboring precursor cells for the tooth supporting apparatus. In this study, we examined gene expression of freshly isolated human dental follicle cells during osteogenic differentiation in vitro. These plastic adherent fibroblastic cells express Notch-1, nestin and vimentin. We differentiated dental follicle cells with dexamethasone or insulin-based protocols into membrane-like structures containing mineralizing foci. An analysis of mineralized tissue with atomic force microscopy illustrated a bone and cementum-like structure. A real-time RT-PCR analysis was developed to investigate expression of typical osteoblast or cementoblast related genes during differentiation. Gene expressions of osteocalcin (OCN), bone morphogenic protein (BMP)-2 and nestin were increased during the both differentiation approaches. Our work demonstrates differentiation of dental follicle cells with an insulin-based protocol for the first time.
机译：牙囊是带有外胚层的结缔组织，带有结缔组织的前体细胞。在这项研究中，我们检查了体外分离的人牙囊细胞在成骨分化过程中的基因表达。这些塑料粘附的成纤维细胞表达Notch-1，巢蛋白和波形蛋白。我们将地塞米松或基于胰岛素的方案的牙囊细胞分化成含有矿化灶的膜状结构。用原子力显微镜对矿化组织的分析显示出骨骼和牙骨质样结构。开发了实时RT-PCR分析以研究分化过程中典型成骨细胞或成骨细胞相关基因的表达。在这两种分化方法中，骨钙蛋白（OCN），骨形态发生蛋白（BMP）-2和巢蛋白的基因表达均增加。我们的工作首次展示了基于胰岛素的方案对牙囊细胞的分化。

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