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首页> 外文期刊>Cell biology international. >Variability of ribosomal RNA genes in Rauwolfia species: parallelism between tissue culture-induced rearrangements and interspecies polymorphism.
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Variability of ribosomal RNA genes in Rauwolfia species: parallelism between tissue culture-induced rearrangements and interspecies polymorphism.

机译:Rauwolfia物种中核糖体RNA基因的变异性:组织培养物引起的重排与种间多态性之间的平行性。

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摘要

An analysis of 18S-25S and 5S rRNA genes in intact plants and cultured tissues of some Rauwolfia species was performed to compare these sequences variability occurred as a result of the species evolution in nature and that induced by tissue culture. The restriction fragment length polymorphism of 18S-25S and 5S rDNA was found both in intact plants of various Rauwolfia species and in long-term Rauwolfia serpentina tissue cultures. In addition, changes in the amount of 18S-25S rRNA genes were observed in long-term R. serpentina tissue cultures. The results demonstrate that rDNA variability observed in intact plants as well as in long-term cultures is attributed to differences in the same regions of ribosomal RNA genes.
机译:对完整的植物和某些紫罗兰属物种的培养组织中的18S-25S和5S rRNA基因进行了分析,以比较这些序列的变异性,这些变异性是自然界物种进化和组织培养诱导的。 18S-25S和5S rDNA的限制性片段长度多态性在各种罗非鱼物种的完整植物中和长期在蛇形罗非鱼组织培养物中均被发现。另外,在长期的蛇纹螺旋体组织培养中观察到18S-25S rRNA基因数量的变化。结果表明,在完整植物以及长期培养物中观察到的rDNA变异性归因于核糖体RNA基因相同区域的差异。

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