Comparison of impact of EMCV replication on the nuclear apparatus of NIH 3T3 and HEp-2 cells.

Karalyan ZA; Jaghatspanyan NG; Gasparyan MH; Hakobyan LA; Abroyan LO; Ter Pogossyan ZR; Kamalyan LA; Karalova EM

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Comparison of impact of EMCV replication on the nuclear apparatus of NIH 3T3 and HEp-2 cells.

机译：比较EMCV复制对NIH 3T3和HEp-2细胞核装置的影响。

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We have investigated differences between the actions of encephalomyocarditis virus (EMCV) on cytometric indices in cultured NIH 3T3 and HEp-2 cells, which are characterized by different levels of transformation. HEp-2 cells surviving 48 h after EMCV infection showed lower nuclear ploidy, reduced nuclear area, fewer nucleoli and a higher percentage of euploid cells. There was a significant increase of nucleolaruclear DNA 6-24 h after EMCV infection. However, EMCV had markedly different effects on NIH 3T3 cells: there was a consistent increase in population ploidy, but the average number of nucleoli and the number of euploid cells in the population remained constant. The nucleolaruclear DNA ratio was almost unchanged. These different viral effects might be explained by the contrasting levels of differentiation of the cultured cell lines. The number of nucleoli does not depend on the amount of nuclear DNA in either viral-infected or intact cells but on the euploidy-to-aneuploidy ratio. The ratio of the sums of the nucleolar perimeters to the nuclear perimeter increases linearly with the number of nucleoli per nucleus in both intact and virus-infected cells. In both cell lines, the amount of DNA per nucleolus decreases as the number of nucleoli increases.

机译：我们研究了脑心肌炎病毒（EMCV）对培养的NIH 3T3和HEp-2细胞的细胞计数指标的作用之间的差异，这些差异的特征在于不同的转化水平。在EMCV感染后存活48小时的HEp-2细胞显示出较低的核倍性，减少的核面积，较少的核仁和较高的整倍体细胞百分比。在EMCV感染后6-24小时，核仁/核DNA显着增加。但是，EMCV对NIH 3T3细胞的作用明显不同：群体倍性不断增加，但群体中核仁的平均数目和整倍体细胞的数目保持恒定。核仁/核DNA的比例几乎没有变化。这些不同的病毒作用可以通过培养的细胞系分化水平的对比来解释。核仁的数量不取决于病毒感染或完整细胞中核DNA的数量，而是取决于整倍体与非整倍体的比率。在完整的和感染病毒的细胞中，核仁周长与核周长之比随每个核中核仁数线性增加。在两种细胞系中，每个核仁的DNA量随着核仁数量的增加而减少。

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《Cell biology international.》 |2005年第7期|共7页
作者
Karalyan ZA; Jaghatspanyan NG; Gasparyan MH; Hakobyan LA; Abroyan LO; Ter Pogossyan ZR; Kamalyan LA; Karalova EM;
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正文语种 eng
中图分类细胞生物学;
关键词
Encephalomyocarditis virus; DNA; replication; Viral;

机译：脑心肌炎病毒;DNA;复制;病毒;

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1. Comparison of impact of EMCV replication on the nuclear apparatus of NIH 3T3 and HEp-2 cells. [J] . Karalyan ZA, Jaghatspanyan NG, Gasparyan MH, Cell biology international. . 2005,第7期

机译：比较EMCV复制对NIH 3T3和HEp-2细胞核装置的影响。
2. Murine FGF-inducible kinase is rapidly degraded via the nuclear ubiquitin-proteosome system when overexpressed in NIH 3T3 cells. [J] . Alberts GF, Winkles JA Cell cycle . 2004,第5期

机译：当NIH 3T3细胞中过表达时，鼠FGF诱导型激酶通过核泛素-蛋白体系统迅速降解。
3. Glycoprotein isolated from Ulmus davidiana Nakai inhibits TPA-induced apoptosis through nuclear factor-kappa B in NIH/3T3 cells. [J] . Lee SJ, Heo KS, Oh PS, Toxicology Letters: An International Journal Providing a Forum for Original and Pertinent Contributions in Toxicology Research . 2004,第2期

机译：分离自Namus alvid davidiana的糖蛋白通过NIH / 3T3细胞中的核因子-κB抑制TPA诱导的凋亡。
4. Comparison of WI-38 and NIH/3T3 Cells as Standards for the Evaluation of the Inhibition of Pancreatic Cancer Cell Lines by Organotin Polymers Containing Histamine and Phentolamine [C] . Michael R. Roner, Charles E. Carraher, Alisa Moric, American Chemical Society., Division of Polymeric Materials : Science and Engineering., Meeting . 2013

机译：WI-38和NIH / 3T3细胞作为含有组胺和芬兰的有机素聚合物评估胰腺癌细胞系抑制的标准的标准
5. Vav3 and Rho GTPases, involved in the regulation of cell transformation, migration and invasion in NIH 3T3 fibroblasts and prostate cancer cells. [D] . Gong, Jianli. 2007

机译：Vav3和Rho GTPases参与NIH 3T3成纤维细胞和前列腺癌细胞的细胞转化，迁移和侵袭的调控。
6. Core binding factor beta-smooth muscle myosin heavy chain chimeric protein involved in acute myeloid leukemia forms unusual nuclear rod-like structures in transformed NIH 3T3 cells. [O] . C Wijmenga, P E Gregory, A Hajra, 1996

机译：参与急性髓性白血病的核心结合因子β-平滑肌肌球蛋白重链嵌合蛋白在转化的NIH 3T3细胞中形成异常的核棒状结构。
7. Core binding factor beta-smooth muscle myosin heavy chain chimeric protein involved in acute myeloid leukemia forms unusual nuclear rod-like structures in transformed NIH 3T3 cells. [O] . Wijmenga, C, Gregory, P E, Hajra, A, 1996

机译：参与急性髓细胞白血病的核心结合因子β-平滑肌肌球蛋白重链嵌合蛋白在转化的NIH 3T3细胞中形成异常的核棒状结构。
8. Comparison of the Radiological Impacts of Thorium and Uranium Nuclear Fuel Cycles. [R] . Meyer, H. R., Witherspoon, J. P., McBride, J. P., 1982

机译：钍和铀核燃料循环放射性影响的比较。

Comparison of impact of EMCV replication on the nuclear apparatus of NIH 3T3 and HEp-2 cells.

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