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首页> 外文期刊>Cell biology international. >Immunological identification of types I and III collagen in bovine lens epithelium and its anterior lens capsule.
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Immunological identification of types I and III collagen in bovine lens epithelium and its anterior lens capsule.

机译：牛晶状体上皮及其晶状体前囊中I型和III型胶原的免疫学鉴定。

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摘要

To define the molecular structure of bovine lens epithelium and its anterior lens capsule, we investigated the composition of lens capsule basement membrane proteins. Immunofluorescence and immunogold techniques were used to demonstrate the presence of type I and type III collagen in the lens capsule and in primary explant epithelial cultures grown on protein-binding membranes. Immunofluorescence staining with specific antibodies indicated that type I and type III collagen were constituents of lens basement membrane. We observed that deposition of type III collagen was more than type I collagen. The synthesis of fibrillar collagen by lens epithelium and its deposition in the lens capsule was established by localization of fibrillar collagen by transmission immunoelectron microscopy. These results demonstrate for the first time that normal lens epithelium synthesize fibrillar collagen which is an intrinsic component of the anterior lens capsule basement membrane.
机译：为了确定牛晶状体上皮及其前晶状体囊的分子结构，我们研究了晶状体囊基底膜蛋白的组成。免疫荧光和免疫金技术用于证明晶状体囊膜和蛋白结合膜上生长的原代外植体上皮培养物中I型和III型胶原蛋白的存在。用特异性抗体进行的免疫荧光染色表明I型和III型胶原是晶状体基底膜的成分。我们观察到，III型胶原蛋白的沉积超过了I型胶原蛋白。晶状体上皮纤维状胶原的合成及其在晶状体囊中的沉积是通过透射免疫电子显微镜对纤维状胶原的定位来确定的。这些结果首次证明正常晶状体上皮合成纤维状胶原，其是晶状体前囊基底膜的固有成分。

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