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首页> 外文期刊>Cell biology international. >Hydrogen peroxide increases the activities of soxRS regulon enzymes and the levels of oxidized proteins and lipids in Escherichia coli.
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Hydrogen peroxide increases the activities of soxRS regulon enzymes and the levels of oxidized proteins and lipids in Escherichia coli.

机译：过氧化氢可增加大肠杆菌中soxRS regulon酶的活性以及氧化蛋白和脂质的水平。

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摘要

The effects of hydrogen peroxide treatments on Escherichia coli KS400 and AB1157 cells were assessed by monitoring the accumulation of oxidative damage products, carbonyl proteins and thiobarbituric acid-reactive substances (TBARS), as well as the activities of selected antioxidant enzymes. H(2)O(2) treatment stimulated increases in both TBARS and carbonyl protein levels in dose- and time-dependent manners in KS400 cells. The accumulation of TBARS was much more variable with H(2)O(2) treatment; TBARS content was significantly increased in response to 5 microM H(2)O(2), whereas a significant increase in carbonyl protein content occurred at 100 microM H(2)O(2). Similarly, treatment with 20 microM hydrogen peroxide for different lengths of time resulted in peak TBARS accumulation by 20 min, whereas carbonyl protein levels were significantly elevated only after 60 min. In AB1157 cells, treatment with 20 microM hydrogen peroxide for 20 min led to strong increases in both carbonyl protein and TBARS levels. This treatment also triggered increased activities of enzymes of the oxyR regulon (catalase, peroxidase, and glutathione reductase) in both strains. In the AB1157 strain, H(2)O(2) exposure also increased the activities of two enzymes of the soxRS regulon (superoxide dismutase and glucose-6-phosphate dehydrogenase) by 50-60%. The data show differential variability of lipids versus proteins to oxidative damage induced by H(2)O(2,) as well as strain-specific differences in the accumulation of damage products and the responses by antioxidant enzymes to H(2)O(2) stress.
机译：通过监测氧化损伤产物，羰基蛋白和硫代巴比妥酸反应性物质（TBARS）的积累以及所选抗氧化剂的活性，评估了过氧化氢处理对大肠杆菌KS400和AB1157细胞的影响。 H（2）O（2）治疗在KS400细胞中以剂量和时间依赖性方式刺激了TBARS和羰基蛋白水平的增加。 TBARS的积累与H（2）O（2）处理相比变化更大。 TBARS含量显着增加响应5 microM H（2）O（2），而羰基蛋白质含量显着增加发生在100 microM H（2）O（2）。同样，用20 microM过氧化氢处理不同时间长度会导致20分钟内TBARS达到峰值积累，而羰基蛋白水平仅在60分钟后才显着升高。在AB1157细胞中，用20 microM过氧化氢处理20分钟会导致羰基蛋白和TBARS含量均大幅增加。该处理还触发了两种菌株中oxyR regulon酶（过氧化氢酶，过氧化物酶和谷胱甘肽还原酶）的活性增加。在AB1157菌株中，H（2）O（2）的暴露也使soxRS调节剂的两种酶（超氧化物歧化酶和葡萄糖6-磷酸脱氢酶）的活性增加了50-60％。数据显示脂质与蛋白质对H（2）O（2）诱导的氧化损伤的差异变异性，以及损伤产物积累和抗氧化酶对H（2）O（2）的响应中的应变特异性差异）压力。

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