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首页> 外文期刊>Cell biology international. >The nucleocytoplasmic microfilament network in protoplasts from cultured soybean cells is a plastic entity that pervades the cytoplasm except the central vacuole.
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The nucleocytoplasmic microfilament network in protoplasts from cultured soybean cells is a plastic entity that pervades the cytoplasm except the central vacuole.

机译：来自培养的大豆细胞的原生质体中的核质微丝网络是一个塑料实体，除了中央液泡外，它遍布细胞质。

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The microfilament network of cultured Glycine max cells (SB-1 line), and protoplasts was visualized with rhodamine-phalloidin under conditions that lysed the protoplast and changed the cell shape. The whole cell had the typical microfilament distribution of a "cage" around the nucleus, from which the large subcortical cables and transvacuolar strands radiated towards the cortex until it reached the cortical microfilament network. Upon cell wall removal, the network conserved its compartmentalization. Thus, the redistribution of the shape where the vacuole becomes a central entity, made the cytoplasm displace peripherally, but the network distribution was conserved. When protoplasts were lysed in a low osmotic medium, the vacuoles were gradually released intact. Under these conditions, the F-actin staining remained within the ghost of the cell, but none was detected in either emerging or almost completely released vacuoles. Most of the released F-actin was found in debris from the cell lysate in the form of microfilaments. When the ghosts were constrained in a coverslip with an air bubble, the shape of the ghost changed accordingly, but the microfilament network distribution remained constant. These results provide further evidence that the vacuole of plant cells does not have detectable associated F-actin. In addition, we demonstrate that the actin microfilament network is a moldable entity that can change its shape but keeps its distribution under constant conditions, in these cultured cells.
机译：在裂解原生质体并改变细胞形状的条件下，用若丹明-鬼笔环肽可视化培养的大豆最大细胞（SB-1系）和原生质体的微丝网络。整个细胞在核周围有一个典型的“笼子”微丝分布，大的皮层下电缆和透泡链从那里向皮质辐射，直到到达皮质微丝网络。除去细胞壁后，网络保留了其分隔性。因此，液泡成为中心实体的形状的重新分布使细胞质在外周移位，但网络分布得以保留。当原生质体在低渗透介质中裂解时，液泡逐渐完整释放。在这些条件下，F-肌动蛋白染色仍保留在细胞的阴影中，但在出现的或几乎完全释放的液泡中均未检测到。在细胞裂解物中的碎片中，以微丝的形式发现了大多数释放的F-肌动蛋白。当将鬼影约束在带有气泡的盖玻片中时，鬼影的形状相应地发生了变化，但是微丝网络的分布却保持不变。这些结果提供了进一步的证据，表明植物细胞的液泡没有可检测的相关F-肌动蛋白。另外，我们证明了肌动蛋白微丝网络是可模制的实体，可以在这些培养的​​细胞中改变其形状，但在恒定条件下保持其分布。

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