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首页> 外文期刊>Cell biology international. >NO CHANGE IN BONE-SPECIFIC ALKALINE PHOSPHATASE ACTIVITIES IN CULTURED RAT OSTEOBLASTIC CELLS UNDER L-ASCORBATE AND BETA-GLYCEROPHOSPHATE-INDUCED MINERALIZATION
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NO CHANGE IN BONE-SPECIFIC ALKALINE PHOSPHATASE ACTIVITIES IN CULTURED RAT OSTEOBLASTIC CELLS UNDER L-ASCORBATE AND BETA-GLYCEROPHOSPHATE-INDUCED MINERALIZATION

机译：L-抗坏血酸和β-草甘膦诱导的矿化作用对培养的大鼠成骨细胞中碱性磷酸酶活性的影响没有变化

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摘要

Mineralization occurred both in fetal rat calvarial cells and UMR 106 osteoblastic cells when they were cultured in medium containing L-ascorbate and beta-glycerophosphate as evidenced by von Kossa staining as well as deposition of calcium ions and inorganic phosphate in the cells. When compared with corresponding non-mineralized cell cultures, both the mineralized cultures of calvarial cells and UMR 106 cells did not exhibit any change in intracellular bone-specific alkaline phosphatase activities which were measured by wheatgerm lectin precipitation method. Our results support the hypothesis that mineralization may not exert any direct negative feedback on matrix protein synthesis in osteoblasts during bone formation.
机译：当它们在含有L-抗坏血酸盐和β-甘油磷酸的培养基中培养时，胎儿大鼠颅盖细胞和UMR 106成骨细胞中都发生矿化作用，这由von Kossa染色以及钙离子和无机磷酸盐在细胞中的沉积证明。当与相应的非矿化细胞培养物比较时，颅骨细胞和UMR 106细胞的矿化培养物均未显示出通过小麦胚芽凝集素沉淀法测定的细胞内骨特异性碱性磷酸酶活性的任何变化。我们的结果支持以下假设：矿化可能不会对成骨细胞在骨形成过程中的基质蛋白合成施加任何直接的负反馈。

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