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首页> 外文期刊>Cell biology international. >PROLIFERATION OF CHICK PRIMORDIAL GERM CELLS CULTURED ON STROMA CELLS FROM THE GERMINAL RIDGE
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PROLIFERATION OF CHICK PRIMORDIAL GERM CELLS CULTURED ON STROMA CELLS FROM THE GERMINAL RIDGE

机译：在初乳中，在基质细胞上培养的雏形胚生殖细胞的增殖

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摘要

Fluorescent reagent-labelled PGCs isolated from the blood of 2-day-old chick embryos were cultured on stroma cells derived from 5-day-old germinal ridge in Medium 199 supplemented with 10% FBS, human IGF-1, bovine FGF-b, and murine LIF. In 7 experiments, the number of MCs increased by an average of 4.8 fold in 4 days. Intrinsic PGCs in the 5-day embryonic germinal ridge were observed loosely attached to the stroma cells, and they also increased 3.8 fold during culture for 4 days. These results indicate the possibility of applying this culture method to the production of transgenic chickens.
机译：在补充有10％FBS，人IGF-1，牛FGF-b的199培养基中，将从2天大的鸡胚血液中分离出的荧光试剂标记的PGC培养在5天大的生发脊的基质细胞上。和鼠类LIF。在7个实验中，MC的数量在4天内平均增加了4.8倍。观察到在5天胚胎生发脊中的内在PGC松散地附着在基质细胞上，并且在培养4天的过程中它们也增加了3.8倍。这些结果表明将该培养方法应用于转基因鸡的生产的可能性。

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