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首页> 外文期刊>Cell biology international. >GERM LINE CHIMERA PRODUCED BY TRANSFER OF CULTURED CHICK PRIMORDIAL GERM CELLS
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GERM LINE CHIMERA PRODUCED BY TRANSFER OF CULTURED CHICK PRIMORDIAL GERM CELLS

机译：通过培养小鸡原代生殖细胞产生的生殖系化学表达

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Intrinsic primordial germ cells (PGCs) from stage 27 (5-day-old) chick embryonic germinal ridges were cultured in vitro for a further 5 days, and shown to proliferate on stroma cells derived from the germinal ridge. To determine whether these cultured PGCs could colonize and contribute to the germ-line, PGCs were isolated by gentle pipetting, labeled with PKH26 fluorescent dye and injected into the blood stream of stage 17 (2.5-day-old) chick embryos. The recipient embryos were incubated until they reached stage 28. Thin sections of these embryos were analysed by fluorescent confocal laser microscopy. These analyses showed that the labeled donor PGCs had migrated into the germinal ridges of the recipient embryos, and transplanted PGCs had undergone at least 3-7 divisions. These results suggest that PGCs that had passed far beyond the migration stage in vivo were still able to migrate, colonize and proliferate in recipient chick embryonic gonads.
机译：将来自第27期（5天大）雏鸡胚胎生发脊的内源性原始生殖细胞（PGC）在体外再培养5天，并显示出在源自该发脊的基质细胞上增殖。为了确定这些培养的​​PGC是否能定殖并有助于种系，通过轻轻移液将PGC分离，用PKH26荧光染料标记，然后注入第17期（2.5天大）雏鸡胚胎的血流中。将受体胚胎孵育直至达到第28阶段。通过荧光共聚焦激光显微镜分析这些胚胎的薄片。这些分析表明标记的供体PGC已经迁移到受体胚胎的生发脊中，并且移植的PGC经历了至少3-7个分裂。这些结果表明，已经远远超过了体内迁移阶段的PGC仍然能够在受体雏鸡性腺中迁移，定植和增殖。

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