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首页> 外文期刊>Cell biology international. >ACETYLATION OF ALPHA-TUBILIN IN DIFFERENT BOVINE CELL TYPES - IMPLICATIONS FOR MICROTUBULE DYNAMICS IN INTERPHASE AND MITOSIS
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ACETYLATION OF ALPHA-TUBILIN IN DIFFERENT BOVINE CELL TYPES - IMPLICATIONS FOR MICROTUBULE DYNAMICS IN INTERPHASE AND MITOSIS

机译：牛小管素在不同类型牛细胞中的乙酰化作用-对微管动力学的影响

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Previous work on five cell types isolated from the bovine corpus luteum showed that the mass of acetylated microtubules (acet-MTs) in interphase differed. Endothelial cells, termed type 3, showed few acet-MTs, whereas the interphase cytoskeleton of granulosal-like cells, termed type 5, was rich in acet-MTs. lit the present study, these cultured cells were used to determine whether the degree of alpha-tubulin acetylation in interphase had consequences on mitosis. To this end, the distribution of acet-MTs was determined throughout the cell cycle using a monoclonal antibody, 6-11B-1, directed against acetylated alpha-tubulin. For comparison, tyrosinated MTs were visualized with another monoclonal antibody, YL1/2, detecting tyrosinated alpha-tubulin. Although the amount of acet-MTs in interphase differed significantly between both cell types, major differences in the appearance of acet-MTs during mitosis were only apparent in prophase and during transition from late telophase to interphase. Thus, irrespective of different alpha-tubulin acetylation in interphase, spindle structure is uniform. Since acetylation of alpha-tubulin is believed to indicate the presence of relatively stable MTs, we conclude that MT dynamics is differently controlled in interphase and mitosis. Thereby interphase cells are able to carry out functions which involve stable MTs and the cells progress through mitosis in the presence of more dynamic MTs.
机译：从牛黄体分离的五种细胞类型的先前研究表明，相间乙酰化微管（acet-MTs）的质量不同。称为3型的内皮细胞显示很少的acet-MT，而称为5型的颗粒状样细胞的相间细胞骨架富含acet-MT。在本研究中，这些培养的​​细胞用于确定相间α-微管蛋白的乙酰化程度是否对有丝分裂有影响。为此，使用针对乙酰化α-微管蛋白的单克隆抗体6-11B-1确定了整个细胞周期中acet-MT的分布。为了进行比较，用另一种单克隆抗体YL1 / 2观察酪氨酸MT，检测酪氨酸α-微管蛋白。尽管两种细胞类型间期的acet-MTs量存在显着差异，但有丝分裂期间acet-MTs外观的主要差异仅在前期和末期至末期过渡期间才明显。因此，无论相间的α-微管蛋白乙酰化程度如何，纺锤体结构都是均匀的。由于α-微管蛋白的乙酰化被认为表明存在相对稳定的MT，因此我们得出结论，在相间和有丝分裂中，MT动力学受到不同的控制。因此，相间细胞能够执行涉及稳定MT的功能，并且在存在更多动态MT的情况下，细胞通过有丝分裂进行。

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