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首页> 外文期刊>Cell biology international. >SECRETION OF TNF-alpha FROM MACROPHAGES FOLLOWING INDUCTION WITH A LIGNIN DERIVATIVE.
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SECRETION OF TNF-alpha FROM MACROPHAGES FOLLOWING INDUCTION WITH A LIGNIN DERIVATIVE.

机译：用木质素衍生物诱导后，巨噬细胞分泌TNF-α。

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摘要

Macrophages derived from rat bone marrow were treated with macrophage colony, stimulating factor (M-CSF) to obtain a sufficient number of cells for the tumor necrosis factor (TNF-alpha) assay. The present study has been designed to investigate whetherthe production of TNF-a, which induces multinucleated giant celt formation, is regulated by polyanions such as lignin derivatives. ELISA for TNF-a showed that the polyanion induced TNF-alpha production by macrophages. The secretion of TNF-alpha from thecells reached a maximum at 3-6 h, and then showed a slight decline. Northern blotting of TNF-a mRNA showed that the amount of TNF-alpha reached a maximum within 1 h of macrophage culture in the presence of a lignin derivative. On the other hand, TNF-alpha mRNA was undetectable in the control cells. It was concluded that stimuli such as that provided by lignin derivatives increases the amount of TNF-alpha mRNA, which is then followed by translation of TNF-alpha.
机译：用巨噬细胞集落刺激因子（M-CSF）处理源自大鼠骨髓的巨噬细胞，以获得足够数量的细胞用于肿瘤坏死因子（TNF-α）分析。本研究旨在调查TNF-α的产生是否会诱导多核巨细胞形成，并受到诸如木质素衍生物等聚阴离子的调节。 TNF-α的ELISA显示，聚阴离子诱导巨噬细胞产生TNF-α。细胞中TNF-α的分泌在3-6小时达到最大，然后显示出轻微的下降。 TNF-αmRNA的RNA印迹显示，在木质素衍生物存在的情况下，巨噬细胞培养1小时内TNF-α的量达到最大值。另一方面，在对照细胞中未检测到TNF-αmRNA。得出的结论是，诸如木质素衍生物提供的刺激会增加TNF-αmRNA的量，然后再翻译TNF-α。

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