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首页> 外文期刊>Cell biology international. >ION TRANSPORT IN PRIMARY CULTURES FROM HUMAN SWEAT GLAND COILS STUDIED WITH X-RAY MICROANALYSIS
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ION TRANSPORT IN PRIMARY CULTURES FROM HUMAN SWEAT GLAND COILS STUDIED WITH X-RAY MICROANALYSIS

机译：X射线显微分析研究人汗腺螺旋原生培养中的离子迁移。

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摘要

Chloride secretion in primary cultures of cells originating from om the secretory coil of human sweat glands was investigated by electron probe X-ray microanalysis. The total intracellular CI concentration was lowered by muscarinic agonists (carbachol and acetylcholine), as well as by the calcium ionophore A23187. The muscarinic agonists also lowered the cellular K concentration. Cl- secretion induced by these agonists could be inhibited by the chloride channel blocker NPPB. After cAMP stimulation, the frequency distribution of the Cl concentration changed from Gaussian to bimodal, indicating that cAMP induces Cl- secretion only from a subpopulation of the cells. Also ATP stimulated Cl- secretion, indicating the presence of purinergic receptors. The results suggest that some of the cells in addition to Ca2+-regulated Cl- channels also possess cAMP-activated Cl- channels. Hence, the primary cultures still possess the Cl- transport mechanisms known to be present in intact glands. It can, however, not be excluded that some coil cells have acquired ductal characteristics during culture.
机译：通过电子探针X射线显微分析研究了源自人类汗腺分泌线圈的细胞原代培养物中的氯化物分泌。毒蕈碱激动剂（咔唑和乙酰胆碱）以及钙离子载体A23187降低了总细胞内CI浓度。毒蕈碱激动剂也降低了细胞的钾浓度。这些激动剂诱导的Cl分泌可被氯离子通道阻滞剂NPPB抑制。在cAMP刺激后，Cl浓度的频率分布从高斯变为双峰，表明cAMP仅从细胞亚群诱导Cl分泌。 ATP也刺激Cl分泌，表明存在嘌呤能受体。结果表明，除了Ca2 +调节的Cl-通道外，某些细胞还具有cAMP激活的Cl-通道。因此，原代培养物仍具有已知存在于完整腺体中的Cl转运机制。但是，不能排除某些线圈细胞在培养过程中具有导管特性。

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