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首页> 外文期刊>Cell biology international. >IS THE RECOVERY OF MICROTUBULE ORIENTATION IN PEA ROOTS DEPENDENT ON THE CELL WALL
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IS THE RECOVERY OF MICROTUBULE ORIENTATION IN PEA ROOTS DEPENDENT ON THE CELL WALL

机译:是依赖于细胞壁的豌豆根中微管取向的恢复

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摘要

This study tested several aspects of a model proposed by Williamson (1990, 1991) in which stresses in plant cell walls, detected by stress-receptive portions of inelastic cellulose microfibrils, orient microtubules via interactions with cell wall-linked transmembrane proteins. Young expanding cells of pea root tips have highly ordered transverse arrays of microtubules oriented perpendicular to the direction of cell expansion. The recovery of these ordered MT arrays after depolymerisation with oryzalin was assessed. It was shown that treating roots with disrupters of microfibril synthesis (2,6-dichlorobenzonitrile and calcofluor white) or the disruption of Arg-Gly-Asp (RGD)-mediated wall-membrane links did not affect the orientation of recovering microtubule arrays. Furthermore, cell wall stresses themselves appeared unnecessary for regeneration of transverse arrays. The relevance of these findings to Williamson's hypothesis is discussed.
机译:这项研究测试了由Williamson(1990,1991)提出的模型的几个方面,在该模型中,通过无弹性纤维素微纤维的应力接受部分检测到的植物细胞壁应力,通过与细胞壁连接的跨膜蛋白相互作用来定向微管。豌豆根尖的年轻膨胀细胞具有高度有序的微管横向排列,其方向垂直于细胞膨胀的方向。评估了用米杂菌素解聚后这些有序MT阵列的回收率。结果表明,用微纤维合成破坏剂(2,6-二氯苄腈和钙氟白色)处理根或破坏Arg-Gly-Asp(RGD)介导的壁膜连接不会影响回收微管阵列的方向。此外,细胞壁应力本身似乎对于横向阵列的再生是不必要的。讨论了这些发现与威廉姆森假设的相关性。

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