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首页> 外文期刊>Cell biology international. >GENOME SIZE AND CHROMOSOMES IN MARINE SPONGES [SUBERITES DOMUNCULA, GEODIA CYDONIUM]
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GENOME SIZE AND CHROMOSOMES IN MARINE SPONGES [SUBERITES DOMUNCULA, GEODIA CYDONIUM]

机译:海洋海绵中的基因组大小和染色体[大地亚藻类的地下亚线虫]

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摘要

The genome size of the marine sponges Suberites domuncula and Geodia cydonium has been determined by flow cytofluorometric analysis using diamidino-phenylindole [DAPI]. Using human lymphocytes as reference the amount of DNA in cells from S. domuncula has been determined to be 3.7 pg and that of G. cydonium 3.3 pg. While no chromosomes could be identified in G. cydonium, the karyotype of the Suberites domuncula is 32 chromsomes in the diploid state. The size of the chromosomes was between 0.25 and 1.0 mu m. No pronounced banding pattern was visible.
机译:通过使用二mid基苯基吲哚[DAPI]的流式细胞荧光分析确定了海洋海绵紫苏和狗形藻的基因组大小。使用人淋巴细胞作为参考,已确定来自domicula domuncula的细胞中DNA的量为3.7 pg,而犬蓝藻的DNA量为3.3 pg。尽管在C.cydonium中无法鉴定出染色体,但是Suberites domuncula的核型是二倍体状态的32个染色体。染色体的大小在0.25和1.0微米之间。没有明显的条纹图案可见。

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