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首页> 外文期刊>Cell biology international. >RECONSTITUTED HUMAN NORMAL BREAST IN NUDE MICE USING COLLAGEN GEL OF MATRICEL
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RECONSTITUTED HUMAN NORMAL BREAST IN NUDE MICE USING COLLAGEN GEL OF MATRICEL

机译：胶体凝胶胶体重建人体正常乳腺

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Human breast epithelial cells dissociated from reduction mammoplasty specimens were embedded in two commonly used extracellular matrices, type I collagen gel or Matrigel, and subsequently transplanted subcutaneously into athymic nude mice. Histological sections from both types of recovered gels showed epithelial structures arranged as short tubules with-some branching as well as preservation of epithelial cell polarity. Proliferation was studied in vivo by 5-bromo-2'-deoxy-uridine labeling followed by immunostaining of sections from recovered gels. Human breast epithelia embedded in collagen gel or Matrigel had similar prolifemtive activity. Cholera toxin, 17 beta-estradiol, and epidermal growth factor, when tested singly, were growth promoting, and in combination 17, beta-estradiol and cholera toxin had an additive effect but 17, beta-estradiol and epidermal growth factor were not additive. Our model system provides a means to study the endocrine control of normal human breast development.
机译：从还原乳腺成形术标本中分离出来的人乳腺上皮细胞被包埋在两种常用的细胞外基质中，即I型胶原凝胶或基质胶，然后皮下移植到无胸腺裸鼠中。两种类型的回收凝胶的组织学切片均显示上皮结构排列成具有一些分支的短小管，并保留了上皮细胞的极性。通过5-溴-2'-脱氧尿苷标记在体内研究增殖，然后对回收凝胶的切片进行免疫染色。嵌入胶原凝胶或基质胶中的人乳房上皮细胞具有相似的增殖活性。单独测试时，霍乱毒素，17β-雌二醇和表皮生长因子具有促进生长的作用，在组合17中，β-雌二醇和霍乱毒素具有累加作用，而17-β-雌二醇和表皮生长因子则无累加作用。我们的模型系统提供了研究正常人乳房发育的内分泌控制的方法。

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