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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Cytokine chemokine expression in contused rat spinal cord.
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Cytokine chemokine expression in contused rat spinal cord.

机译：挫伤大鼠脊髓中细胞因子趋化因子的表达。

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摘要

Spinal cord injury within the first few hours, is complicated by inflammatory mechanisms, including the influx of monocyte/macrophages as well as the activation of resident spinal microglia and astrocytes. Numerous studies have suggested that the initial infiltration of the hematogenous cells may be due to the secretion of cytokines and chemokines in the injured CNS. In order to elucidate which chemotactic factors may be expressed following traumatic spinal cord contusion, the presence of mRNA for a number of cytokines, chemokines and growth factors was examined in contused rat spinal cord by reverse transcriptase-polymerase chain reaction and immunohistochemistry. Spinal injury was accompanied by an increase in glial fibrillary acidic protein mRNA suggesting astrocyte activation and astrogliosis. TNFalpha message levels were upregulated as early as 1 h post injury and returned to baseline levels by 3 days post injury (DPI). By immunocytochemistry, staining for TNFalpha increased at 1 and 3 dpi and was predominantly diffuse in the necrotic tissue. The chemokines IP-10, MCP-1, and MIP-1alpha were also detected in the injured spinal cord. mRNA levels of IP-10 peaked around 6 h post injury and were upregulated up to 7 dpi. MCP-1 mRNA was detected at 1 h post injury and its levels returned to baseline by 14 dpi. An increase in MCP-1 staining was observed from 1 to 7 dpi. The staining was also diffuse in the necrotic tissue and also localized to cells near the site of injury. The presence of aFGF and bFGF was also detected in the injured spinal cord. mRNA for aFGF was detected at 0 time, increased at 6 h post injury, peaked at 3 days, and remained elevated up to 21 days. bFGF mRNA was initially detected at 1 h post injury, increased between 6 h and 3 days, declined thereafter and returned to baseline levels by 21 days.
机译：在最初的几个小时内，脊髓损伤会因炎症机制而复杂化，包括单核细胞/巨噬细胞的大量涌入以及驻留的脊髓小胶质细胞和星形胶质细胞的活化。大量研究表明，血源性细胞的最初浸润可能是由于受损的CNS中细胞因子和趋化因子的分泌所致。为了阐明在创伤性脊髓挫伤后可能表达哪些趋化因子，通过逆转录酶-聚合酶链反应和免疫组织化学检查了挫伤大鼠脊髓中多种细胞因子，趋化因子和生长因子的mRNA的存在。脊柱损伤伴随着胶质纤维酸性蛋白mRNA的增加，提示星形胶质细胞活化和星形胶质细胞增生。 TNFalpha信息水平最早在受伤后1小时被上调，并在受伤后3天（DPI）恢复到基线水平。通过免疫细胞化学，TNFα的染色在1和3 dpi时增加，并且主要在坏死组织中扩散。在受损的脊髓中也检测到了趋化因子IP-10，MCP-1和MIP-1alpha。 IP-10的mRNA水平在受伤后约6小时达到峰值，并被上调至7 dpi。受伤后1小时检测到MCP-1 mRNA，并且其水平在14 dpi后恢复到基线。从1到7 dpi观察到MCP-1染色增加。染色在坏死组织中也弥散，并且也位于损伤部位附近的细胞中。在受伤的脊髓中也检测到aFGF和bFGF的存在。在第0天检测到aFGF的mRNA，在受伤后6小时增加，在第3天达到峰值，直到21天仍然升高。 bFGF mRNA最初在受伤后1小时被检测到，在6小时至3天之间增加，此后下降，并在21天后恢复到基线水平。

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