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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >5-Hydroxytryptamine2B receptors stimulate Ca2+ increases in cultured astrocytes from three different brain regions.
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5-Hydroxytryptamine2B receptors stimulate Ca2+ increases in cultured astrocytes from three different brain regions.

机译：5-羟色胺2B受体刺激来自三个不同大脑区域的星形胶质细胞中Ca2 +的增加。

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摘要

The expression of 5-hydroxytryptamine-2B (5-HT2B) receptor mRNA has recently been shown in cultured astrocytes. Here the expression of functional 5-HT2B receptors has been studied in cultured astrocytes from rat cerebral cortex, hippocampus, and brain stem. Fluo-3- and fura-2-based microspectrofluorometry was used for measuring changes in intracellular free calcium concentrations ([Ca2+]i). The 5-HT2B agonist alpha-methyl 5-HT (40 nM) produced rapid transient increases in [Ca2+]i in astrocytes from all three brain regions studied, and these responses were blocked by the selective 5-HT2B antagonist rauwolscine (1 microM). The specificity of the responses to alpha-methyl 5-HT was further demonstrated by the failure of 4-(4-fluorobenzoyl)-1-(4-phenylbutyl)-piperidine oxalate (1 microM), a specific 5-HT2A/5-HT2C antagonist, to block these responses. The 5-HT2B-induced increases in [Ca2+]i persisted in Ca2+-free buffer, indicating that the increase in [Ca2+]i results from mobilization of intracellular Ca2+ stores. The expression of 5-HT2B receptors on astroglial cells was further verified immunohistochemically and by Western blot analysis. These results provide evidence of the existence of 5-HT2B receptors on astrocytes in primary culture.
机译：最近在培养的星形胶质细胞中显示了5-羟色胺-2B（5-HT2B）受体mRNA的表达。在这里，已经研究了在大鼠大脑皮层，海马和脑干培养的星形胶质细胞中功能性5-HT2B受体的表达。基于Fluo-3-和fura-2-的微光谱荧光法用于测量细胞内游离钙浓度（[Ca2 +] i）的变化。 5-HT2B激动剂α-甲基5-HT（40 nM）在研究的所有三个大脑区域的星形胶质细胞中产生[Ca2 +] i快速瞬时增加，并且这些反应被选择性5-HT2B拮抗剂rauwolscine（1 microM）阻断。对4-甲基（4-氟苯甲酰基）-1-（4-苯基丁基）-哌啶草酸酯（1 microM），一种特定的5-HT2A / 5-的失败进一步证明了对α-甲基5-HT反应的特异性。 HT2C拮抗剂，以阻断这些反应。 5-HT2B诱导的[Ca2 +] i的增加在无Ca2 +的缓冲液中持续存在，这表明[Ca2 +] i的增加是由于细胞内Ca2 +存储动员的结果。免疫组化和Western印迹分析进一步证实了星形胶质细胞上5-HT2B受体的表达。这些结果提供了在原代培养中星形胶质细胞上存在5-HT2B受体的证据。

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