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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Expression of interleukin-1 alpha, tumor necrosis factor alpha and interleukin-6 genes in astrocytes under ischemic injury.
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Expression of interleukin-1 alpha, tumor necrosis factor alpha and interleukin-6 genes in astrocytes under ischemic injury.

机译：缺血性损伤星形胶质细胞中白细胞介素-1α，肿瘤坏死因子α和白介素-6基因的表达。

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Astrocytes form an integral part of the blood brain barrier and are the first cell type in the central nervous system to encounter insult if there is an ischemic attack. The immunologic reaction of astrocytes to an ischemic insult would be affective to the subsequent responses of other nerve cells. We previously showed that ischemia caused an increase in the levels of interleukin 1alpha (IL-1alpha), tumor necrosis factor alpha (TNF alpha), and interleukin 6 (IL-6) in the culture medium of mouse cerebral cortical astrocyte. We did not have evidence on the source of these cytokines. This study aimed to investigate the expressions of these cytokine mRNAs in the astrocytes under ischemia. Results demonstrated that ischemia could induce necrosis and apoptosis in astrocytes. By using the RT-PCR method, we demonstrated for the first time that the mRNA levels of IL-1alpha, TNF alpha and IL-6 in normal astrocyte was very low, but their expressions could be induced quickly under ischemia. These cytokines might be interactive as indicated by the difference in time course of their expressions, with IL-1alpha being the earliest and IL-6 being the latest. The result provided some understanding of the induction and progression of these immunologic responses in astrocytes under ischemia. It also supported our previous findings that astrocytes contributed to the cytokines released under ischemia.
机译：星形胶质细胞是血脑屏障的组成部分，是中枢神经系统中第一种在发生缺血性发作时会受到侮辱的细胞类型。星形胶质细胞对缺血性损伤的免疫反应将影响其他神经细胞的后续反应。我们以前表明缺血导致小鼠脑皮质星形胶质细胞培养基中白介素1α（IL-1alpha），肿瘤坏死因子α（TNF alpha）和白介素6（IL-6）的水平升高。我们没有这些细胞因子来源的证据。这项研究旨在调查缺血下星形胶质细胞中这些细胞因子mRNA的表达。结果表明，缺血可以诱导星形胶质细胞坏死和凋亡。通过RT-PCR方法，我们首次证明正常星形胶质细胞中IL-1α，TNFα和IL-6的mRNA水平很低，但是在缺血下可以迅速诱导它们的表达。这些细胞因子可能是相互作用的，如它们的表达时程不同所表明，IL-1alpha最早，IL-6最晚。结果提供了对缺血下星形胶质细胞中这些免疫应答的诱导和进展的一些理解。这也支持了我们先前的发现，即星形胶质细胞促成缺血下释放的细胞因子。

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