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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Stimulation by neurotensin of dopamine and 5-hydroxytryptamine (5-HT) release from rat prefrontal cortex: possible role of NTR1 receptors in neuropsychiatric disorders.
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Stimulation by neurotensin of dopamine and 5-hydroxytryptamine (5-HT) release from rat prefrontal cortex: possible role of NTR1 receptors in neuropsychiatric disorders.

机译：多巴胺和5-羟色胺（5-HT）的神经降压素刺激大鼠前额叶皮层释放：NTR1受体在神经精神疾病中的可能作用。

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摘要

The modulation of cortical dopaminergic and serotonergic neurotransmissions by neurotensin (NT) was studied by measuring the release of dopamine (DA) and 5-hydroxytryptamine (5-HT) from the prefrontal cortex (PFC) of freely moving rats. The samples were collected via transversal microdialysis. Dopamine and 5-HT levels in the dialysate were measured using high-performance liquid chromatography (HPLC) with an electrochemical detector. Local administration of neurotensin (1microM or 0.1microM) in the PFC via the dialysis probe produced significant, long-lasting, and concentration-dependent increase in the extracellular release of DA and 5-HT. The increase produced by 1microM neurotensin reached a maximum of about 210% for DA and 340% for 5-HT. A high-affinity selective neurotensin receptor (NTR1) antagonist {2-[(1-(7-chloro-4-quinolinyl)-5-(2,6-dimethoxyphenyl)pyrazol-3yl)carbonylamino tricyclo (3.3.1.1.(3.7)) decan-2-carboxylic acid} (SR 48692), perfused locally at a concentration of 0.1microM and 0.5microM in the PFC antagonized the effects of 1microM neurotensin. Our in vivo neurochemical results indicate, for the first time, that neurotensin is able to regulate cortical dopaminergic and serotonergic neuronal activity in freely moving rats. These effects are possibly mediated by interactions of neurotensin with neurons releasing DA or 5-HT, projecting to the PFC from the ventrotegmental area (VTA) and from the dorsal raphe nuclei (DRN), respectively. The potentiating effects of neurotensin on DA and 5-HT release in the PFC are regulated by NTR1 receptors, probably located on dopaminergic and serotonergic nerve terminals or axons.
机译：通过测量自由运动大鼠前额叶皮层（PFC）中多巴胺（DA）和5-羟色胺（5-HT）的释放，研究了神经降压素（NT）对皮质多巴胺能和5-羟色胺能神经传递的调节作用。通过横向微透析收集样品。使用带有电化学检测器的高效液相色谱（HPLC）测量透析液中的多巴胺和5-HT含量。通过透析探针在PFC中局部施用神经降压素（1microM或0.1microM）在DA和5-HT的细胞外释放中产生明显，持久且浓度依赖性的增加。 1microM神经降压素产生的增加最大程度是DA约为210％，5-HT高达340％。高亲和力选择性神经降压素受体（NTR1）拮抗剂{2-[（1-（7-氯-4-喹啉基）-5-（2,6-二甲氧基苯基）吡唑-3基）羰基氨基三环（3.3.1.1。（3.7 ））decan-2-羧酸}（SR 48692），在PFC中以0.1microM和0.5microM的浓度局部灌注，拮抗1microM神经降压素的作用。我们的体内神经化学结果首次表明，神经降压素能够调节自由运动大鼠的皮质多巴胺能和血清素能神经元活性。这些作用可能是由神经降压素与释放DA或5-HT的神经元相互作用介导的，DA或5-HT分别从腹膜区（VTA）和背脊核（DRN）投射到PFC。神经降压素对PFC中DA和5-HT释放的增强作用受NTR1受体的调节，该受体可能位于多巴胺能和血清素能神经末梢或轴突上。

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