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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Mitochondrial malic enzyme activity is much higher in mitochondria from cortical synaptic terminals compared with mitochondria from primary cultures of cortical neurons or cerebellar granule cells.
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Mitochondrial malic enzyme activity is much higher in mitochondria from cortical synaptic terminals compared with mitochondria from primary cultures of cortical neurons or cerebellar granule cells.

机译：与来自皮层神经元或小脑颗粒细胞原代培养的线粒体相比，皮层突触末端的线粒体中的线粒体苹果酶活性高得多。

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Most of the malic enzyme activity in the brain is found in the mitochondria. This isozyme may have a key role in the pyruvate recycling pathway which utilizes dicarboxylic acids and substrates such as glutamine to provide pyruvate to maintain TCA cycle activity when glucose and lactate are low. In the present study we determined the activity and kinetics of malic enzyme in two subfractions of mitochondria isolated from cortical synaptic terminals, as well as the activity and kinetics in mitochondria isolated from primary cultures of cortical neurons and cerebellar granule cells. The synaptic mitochondrial fractions had very high mitochondrial malic enzyme (mME) activity with a Km and a Vmax of 0.37 mM and 32.6 nmol/min/mg protein and 0.29 mM and 22.4 nmol/min mg protein, for the SM2 and SM1 fractions, respectively. The Km and Vmax for malic enzyme activity in mitochondria isolated from cortical neurons was 0.10 mM and 1.4 nmol/min/mg protein and from cerebellar granule cells was 0.16 mM and 5.2 nmol/min/mg protein. These data show that mME activity is highly enriched in cortical synaptic mitochondria compared to mitochondria from cultured cortical neurons. The activity of mME in cerebellar granule cells is of the same magnitude as astrocyte mitochondria. The extremely high activity of mME in synaptic mitochondria is consistent with a role for mME in the pyruvate recycling pathway, and a function in maintaining the intramitochondrial reduced glutathione in synaptic terminals.
机译：脑中大多数苹果酸酶活性都存在于线粒体中。这种同功酶可能在丙酮酸循环途径中起关键作用，该途径利用二羧酸和底物（如谷氨酰胺）提供丙酮酸，以在葡萄糖和乳酸含量较低时维持TCA循环活性。在本研究中，我们确定了从皮质突触末端分离的线粒体的两个亚部分中苹果酸酶的活性和动力学，以及从皮质神经元和小脑颗粒细胞的原代培养物中分离的线粒体的活性和动力学。突触线粒体组分具有很高的线粒体苹果酸酶（mME）活性，SM2和SM1组分的Km和Vmax分别为0.37 mM和32.6 nmol / min / mg蛋白和0.29 mM和22.4 nmol / min mg蛋白。 。从皮层神经元分离的线粒体中苹果酸酶活性的Km和Vmax为0.10 mM和1.4 nmol / min / mg蛋白，从小脑颗粒细胞分离出的线粒体中苹果酸的Km和Vmax为0.16 mM和5.2 nmol / min / mg蛋白。这些数据表明，与培养的皮质神经元的线粒体相比，皮质突触线粒体中的mME活性高度丰富。小脑颗粒细胞中mME的活性与星形胶质细胞线粒体的活性相同。 mME在突触线粒体中的极高活性与mME在丙酮酸循环途径中的作用以及在突触末端维持线粒体内还原型谷胱甘肽的功能相一致。

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