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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Differential expression of hypothalamic CART mRNA in response to body weight change following different dietary interventions.
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Differential expression of hypothalamic CART mRNA in response to body weight change following different dietary interventions.

机译：下丘脑CART mRNA在不同饮食干预下对体重变化的响应差异表达。

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摘要

Cocaine- and amphetamine-regulated transcript (CART) peptide is widely expressed in the hypothalamus and is involved in the central regulation of energy balance. Using in situ hybridization, this study examined the roles of CART peptide in the hypothalamus of diet-induced obese (DIO) or diet-resistant (DR) mice under different dietary interventions including high-fat (HF), low-fat (LF) and pair-feeding (PF) diet for 6 weeks. Pair feeding the energy intake of the DIO and DR mice was used to determine whether there is an inherent difference in baseline CART expression that may cause the DIO and DR phenotypes. The results demonstrated that CART mRNA expression in the hypothalamus of the DIO mice responded differently on the high-fat diet compared to DR mice. The arcuate nucleus and paraventricular nucleus showed a significant reduction in CART mRNA expression in DIO mice compared to DR mice on the HF diet (-19.6%, p=0.019; -26.1%, p=0.003); whilst a profound increase in CART mRNA expression was observed in the dorsomedial nucleus and lateral hypothalamic area (+44.5%, p=0.007; +37.4%, p=0.033). Our study suggests that the decrease of CART mRNA expression in Arc and PVN regions of DIO mice may contribute to the development of high-fat diet-induced obesity. In addition, CART in the dorsomedial nucleus (DM) of hypothalamus and lateral hypothalamus (LH) may be involved in the activation of an orexigenic effect. Since pair feeding of the high-fat diet eliminated both the body weight and CART mRNA differences between the DIO and DR mice, it is likely that their alterations in gene expression were a consequence of their dissimilar body weight levels.
机译：可卡因和苯丙胺调节的转录（CART）肽在下丘脑中广泛表达，并参与能量平衡的中央调节。本研究使用原位杂交技术，在不同饮食干预措施（包括高脂（HF），低脂（LF））下，研究了CART肽在饮食诱导的肥胖（DIO）或饮食抵抗性（DR）小鼠下丘脑中的作用。和双喂（PF）饮食6周。配对喂养DIO和DR小鼠的能量摄入用于确定基线CART表达是否存在固有差异，这可能会导致DIO和DR表型。结果表明，与DR小鼠相比，DIO小鼠下丘脑中CART mRNA表达对高脂饮食的反应不同。与使用HF饮食的DR小鼠相比，弓形核和室旁核在DIO小鼠中的CART mRNA表达显着降低（-19.6％，p = 0.019; -26.1％，p = 0.003）;而在背核和下丘脑外侧区域观察到CART mRNA表达的显着增加（+ 44.5％，p = 0.007; + 37.4％，p = 0.033）。我们的研究表明，DIO小鼠的Arc和PVN区域CART mRNA表达的降低可能有助于高脂饮食诱导的肥胖症的发展。此外，下丘脑背侧核（DM）和下丘脑外侧（LH）的CART可能与致食性作用的激活有关。由于高脂饮食的配对喂养消除了DIO和DR小鼠的体重和CART mRNA差异，因此它们基因表达的改变很可能是其体重水平不同的结果。

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