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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Repeated immobilization stress alters rat hippocampal and prefrontal cortical morphology in parallel with endogenous agmatine and arginine decarboxylase levels.
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Repeated immobilization stress alters rat hippocampal and prefrontal cortical morphology in parallel with endogenous agmatine and arginine decarboxylase levels.

机译：反复的固定应激会改变大鼠海马和前额叶皮层的形态，并与内源性胍丁胺和精氨酸脱羧酶水平平行。

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摘要

Agmatine, an endogenous amine derived from decarboxylation of L-arginine catalyzed by arginine decarboxylase, has been proposed as a neurotransmitter or neuromodulator in the brain. In the present study, we examined whether agmatine has neuroprotective effects against repeated immobilization-induced morphological changes in brain tissues and possible effects of immobilization stress on endogenous agmatine levels and arginine decarboxylase expression in rat brains. Sprague-Dawley rats were subjected to 2h immobilization stress daily for 7 days. This paradigm significantly increased plasma corticosterone levels, and the glutamate efflux in the hippocampus as measured by in vivo microdialysis. Immunohistochemical staining with beta-tubulin III showed that repeated immobilization caused marked morphological alterations in the hippocampus and medial prefrontal cortex that were prevented by simultaneous treatment with agmatine (50mg/kg/day), i.p.). Likewise, endogenous agmatine levels measured by high-performance liquid chromatography in the prefrontal cortex, hippocampus, striatum and hypothalamus were significantly increased by immobilization, as compared to controls. The increased endogenous agmatine levels, ranging from 92 to 265% of controls, were accompanied by a significant increase of arginine decarboxylase protein levels in the same regions. These results demonstrate that the administration of exogenous agmatine protects the hippocampus and medial prefrontal cortex against neuronal insults caused by repeated immobilization. The parallel increase in endogenous brain agmatine and arginine decarboxylase protein levels triggered by repeated immobilization indicates that the endogenous agmatine system may play an important role in adaptation to stress as a potential neuronal self-protection mechanism.
机译：精氨酸，一种由精氨酸脱羧酶催化的L-精氨酸脱羧衍生的内源性胺，已经被提出作为大脑中的神经递质或神经调节剂。在本研究中，我们检查了胍丁胺是否对反复固定化引起的脑组织形态变化具有神经保护作用，以及固定化应激对大鼠脑中内源性胍丁胺水平和精氨酸脱羧酶表达的可能影响。每天对Sprague-Dawley大鼠进行2h固定应激，持续7天。通过体内微透析测量，该范例显着增加了血浆皮质酮水平和海马中的谷氨酸外排。用β-微管蛋白III进行的免疫组织化学染色显示，反复固定可引起海马和前额内侧皮层的明显形态学改变，同时使用胍丁胺（50mg / kg /天）腹膜内注射可预防这种改变。同样，与对照相比，通过高效液相色谱法测定的前额叶皮层，海马，纹状体和下丘脑中的内源胍丁胺水平显着提高。内源性胍丁胺水平的升高，从对照的92％到265％不等，同时在相同区域中精氨酸脱羧酶蛋白水平也显着增加。这些结果表明，外源性胍丁胺的给药保护了海马和前额内侧皮层免受反复固定引起的神经元损伤。反复固定化触发内源性大脑胍丁胺和精氨酸脱羧酶蛋白水平的平行增加表明，作为潜在的神经元自我保护机制，内源性胍丁胺系统可能在适应压力中起重要作用。

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