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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >The flavonoid baicalin counteracts ischemic and oxidative insults to retinal cells and lipid peroxidation to brain membranes.
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The flavonoid baicalin counteracts ischemic and oxidative insults to retinal cells and lipid peroxidation to brain membranes.

机译：黄酮类黄ical苷可抵消视网膜细胞的缺血性和氧化性损伤以及脂质对脑膜的过氧化作用。

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摘要

The purpose of the present study was to determine whether the flavonoid, baicalin is effective at blunting the negative influence of ischemia/reperfusion to the rat retina in situ and of various insults to a transformed retinal ganglion cells (RGC-5 cells) in culture. Baicalin was administered intraperitoneally just before and after an ischemic insult to retina of one eye of a rat. Ischemia was delivered by raising the intraocular pressure above the systolic blood pressure for 50min. Seven days after ischemia, retinas were analysed for the localisation of various antigens. Retinal extracts were also analysed for various mRNAs. Moreover, the content of specific proteins was deduced in retinal and optic nerve extracts. Also, RGC-5 cells in culture were given one of three different insults, light (1000lx for 2 days), hydrogen peroxide (200microM H(2)O(2) for 24h) or serum deprivation (48h) where cell survival and reactive oxygen species (ROS) formation was assayed. Moreover, a lipid peroxidation assay wasused to compare the antioxidant capacity of baicalin with the flavonoid, epigallocatechin gallate (EGCG). Ischemia/reperfusion to the retina affected the localisation of Thy-1 and choline acetyltransferase (ChAT) and the content of various proteins (optic nerve and retina) and mRNAs (retina). Importantly, baicalin statistically blunted most of the effects induced by ischemia/reperfusion. Only the increase in caspase-8 and caspase-3 mRNAs caused by ischemia/reperfusion were unaffected by baicalin treatment. Baicalin also attenuated significantly the negative insult of light, hydrogen peroxide and serum withdrawal to RGC-5 cells. In the lipid peroxidation studies, baicalin was also found to be equally effective as EGCG to act as an antioxidant. Significantly, the negative insult of serum withdrawal on RGC-5 cell survival was blunted by baicalin but not by EGCG revealing the different properties of the two flavonoids.
机译：本研究的目的是确定黄酮类化合物，黄ical苷是否对减轻局部缺血/再灌注对大鼠视网膜的不良影响以及培养物中对转化的视网膜神经节细胞（RGC-5细胞）的各种损伤是否有效。在对大鼠一只眼的视网膜进行缺血性损伤之前和之后，腹膜内施用黄ical苷。通过将眼内压升高至收缩压以上50分钟来递送局部缺血。缺血7天后，分析视网膜中各种抗原的定位。还分析了视网膜提取物的各种mRNA。此外，在视网膜和视神经提取物中推导了特定蛋白质的含量。此外，对培养中的RGC-5细胞进行了三种不同的损伤之一：光照（1000lx，持续2天），过氧化氢（200microM H（2）O（2），持续24h）或血清剥夺（48h），其中细胞存活和反应性测定氧（ROS）的形成。此外，使用脂质过氧化测定法比较黄ical苷与类黄酮，表没食子儿茶素没食子酸酯（EGCG）的抗氧化能力。视网膜缺血/再灌注影响Thy-1和胆碱乙酰转移酶（ChAT）的定位以及各种蛋白质（视神经和视网膜）和mRNA（视网膜）的含量。重要的是，黄ical苷在统计学上减弱了缺血/再灌注诱导的大多数效应。黄ical素治疗仅影响由缺血/再灌注引起的caspase-8和caspase-3 mRNA的增加。黄ical苷还显着减轻了光，过氧化氢和血清撤回对RGC-5细胞的负面影响。在脂质过氧化研究中，黄ical苷也可作为抗氧化剂与EGCG等效。值得注意的是，黄ical苷抑制了血清撤药对RGC-5细胞存活的负面影响，而EGCG却没有，这揭示了两种类黄酮的不同性质。

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