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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Simultaneous measurement of glutamate and dopamine release from isolated guinea pig cochlea.
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Simultaneous measurement of glutamate and dopamine release from isolated guinea pig cochlea.

机译：从分离的豚鼠耳蜗中同时测量谷氨酸和多巴胺的释放。

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摘要

Glutamate is proved to be a neurotransmitter in the mammalian cochlea, transmitting signals between the inner hair cells and the afferent cochlear nerve terminals. The transmission in this synapse is modulated by the lateral olivocochlear efferent fibers by releasing dopamine and other neurotransmitters. This study undertakes to measure simultaneously the release of dopamine and glutamate from isolated guinea pig cochleae. We combined the in vitro microvolume superfusion method, that uses liquid scintillation analysis, to measure [3H]dopamine with high pressure liquid chromatography (HPLC) to determine the glutamate content of the superfusate at rest and during stimulation. The release of both neurotransmitters was significantly increased when electrical field stimulation was applied at a 10 Hz rate. The nonselective sodium-channel inhibitor tetrodotoxin (TTX) at 1 microM completely blocked the effect of stimulation, indicating the neural origin of both dopamine and glutamate. The dopamine receptor antagonist sulpiride at 100 microM and the dopamine receptor agonist bromocriptine at 20 microM did not change the release of glutamate. In contrast, both bromocriptine and sulpiride significantly increased the stimulation-evoked release of dopamine. The effect of sulpiride is most likely due to the blockade of dopamine autoreceptor. Possible explanations why bromocriptine increased the release include: (1) its partional agonist activity; (2) desensitizations of dopamine autoreceptors; or (3) the higher D1 receptor activity of bromocriptine than sulpiride. This study could provide further insights about the role of dopamine and glutamate in cochlear neurotransmission.
机译：谷氨酸被证明是哺乳动物耳蜗的一种神经递质，可在内部毛细胞和传入的耳蜗神经末梢之间传递信号。通过释放多巴胺和其他神经递质，该突触中的传递受侧耳蜗出射纤维的调节。这项研究致力于同时测量分离的豚鼠耳蜗中多巴胺和谷氨酸的释放。我们结合了使用液体闪烁分析的体外微量混合法，以高压液相色谱法（HPLC）测定[3H]多巴胺，以测定静止和刺激过程中超融合产物的谷氨酸含量。当以10 Hz的频率施加电场刺激时，两种神经递质的释放均显着增加。 1 microM的非选择性钠通道抑制剂河豚毒素（TTX）完全阻断了刺激作用，表明多巴胺和谷氨酸的神经起源。 100 microM的多巴胺受体拮抗剂舒必利和20 microM的多巴胺受体激动剂溴隐亭不会改变谷氨酸的释放。相反，溴隐亭和舒必利均明显增加了刺激引起的多巴胺释放。舒必利的作用很可能是由于多巴胺自身受体的阻滞。溴隐亭为什么增加释放的可能解释包括：（1）其局部激动剂活性; （2）多巴胺自身受体脱敏;或（3）溴隐亭的D1受体活性高于舒必利。这项研究可以提供有关多巴胺和谷氨酸在耳蜗神经传递中的作用的进一步见解。

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