...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Biotechnology >Recombinant baculovirus-based multiple protein expression platform for Drosophila S2 cell culture
【24h】

Recombinant baculovirus-based multiple protein expression platform for Drosophila S2 cell culture

机译：基于重组杆状病毒的果蝇S2细胞培养多蛋白表达平台

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A platform for selective and controllable expression of multiple foreign protein types was developed in insect cell culture. Based on the fact that baculovirus cannot replicate in nonpermissive Drosophila melanogaster Schneider line 2 (S2) cells, S2 cells that stably express human erythropoietin (hEPO) under the control of the S2-derived inducible metallothionein (MT) promoter were infected with three types of recombinant baculoviruses, each of which expressed a different fluorescent protein gene under the control of MT promoter. Addition of copper sulfate as an inducer to infected, stably transfected S2 cells resulted in simultaneous expression of hEPO and three fluorescent proteins. Expression profiles and levels of the three induced fluorescent proteins were similar in all single infected cells. Importantly, expression profiles and levels of hEPO were similar in both non-infected and infected cells, indicating that baculovirus expressed recombinant proteins do not adversely affect expression of host cell recombinant proteins. Expressions of the three fluorescent proteins were able to be selectively regulated by altering combination ratios of the three types of recombinant baculoviruses. Collectively, these data indicate that the baculovirus/stably transfected S2 cell system can be successfully used to express multiple foreign proteins in a controlled and selective manner without the burden of additional selection markers. Such a system would be expected to be attractive as a multiple protein expression platform for engineering metabolic or glycosylation pathways.
机译：在昆虫细胞培养中开发了选择性和可控制地表达多种外源蛋白类型的平台。基于杆状病毒不能在非许可的黑腹果蝇施耐德线2（S2）细胞中复制的事实，在S2衍生的诱导型金属硫蛋白（MT）启动子的控制下稳定表达人促红细胞生成素（hEPO）的S2细胞感染了三种类型的重组杆状病毒，每个杆状病毒在MT启动子的控制下表达不同的荧光蛋白基因。将硫酸铜作为诱导剂添加到感染的，稳定转染的S2细胞中，可同时表达hEPO和三种荧光蛋白。在所有单个感染的细胞中，三种诱导的荧光蛋白的表达谱和水平相似。重要的是，在未感染和感染的细胞中，hEPO的表达谱和水平都相似，这表明杆状病毒表达的重组蛋白不会对宿主细胞重组蛋白的表达产生不利影响。三种荧光蛋白的表达能够通过改变三种重组杆状病毒的组合比例来选择性地调节。总体而言，这些数据表明杆状病毒/稳定转染的S2细胞系统可以成功地以受控和选择性的方式成功表达多种外源蛋白，而无其他选择标记的负担。这种系统有望作为用于工程化代谢或糖基化途径的多蛋白表达平台而具有吸引力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号