...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Biotechnology >A semi-immobilizing system associated with microspectrofluorimetric and videoimaging analysis for intracytoplasmic calcium measurement in individual viable spermatozoa
【24h】

A semi-immobilizing system associated with microspectrofluorimetric and videoimaging analysis for intracytoplasmic calcium measurement in individual viable spermatozoa

机译：半固定系统与微光谱荧光和视频影像分析相关，用于单个活精子的胞浆内钙测量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We describe a fast and simple method to monitor variations of intracytoplasmic calcium concentrations in very small and motile cells such as mammalian spermatozoa during measurement time. The method combines a procedure of sperm cells semi-immobilization with microspectrofluorimetric measurement of intracytoplasmic calcium concentrations supported by videoimaging that allows also a constant monitoring of viability during the time of calcium recording. In this paper we show that a semi-immobilization of individual viable spermatozoa can be obtained preparing the agar matrix in a two step procedure. The cell immobilizing system proposed, associated with the microspectrofluorimetric analysis supported by videoimaging, is a simple, rapid and useful tool for those studies having the goal of correlating the presence and cellular distribution of ion channels with their functional status and their response to physiologic and/or pharmacological molecules.
机译：我们描述了一种快速而简单的方法来监测在测量时间内非常小的活动细胞（例如哺乳动物的精子）中胞浆内钙浓度的变化。该方法结合了将精子细胞半固定化的过程与通过视频成像支持的细胞质内钙离子浓度的微光谱荧光测量相结合的方法，该方法还可以在钙记录期间持续监测生存力。在本文中，我们表明可以通过两步操作制备琼脂基质，获得单个活精子的半固定化。所提议的细胞固定系统与视频成像支持的微光谱荧光分析相关联，对于那些旨在将离子通道的存在和细胞分布与其功能状态及其对生理和/或反应相关联的目标的研究而言，是一种简单，快速且有用的工具或药理分子。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号