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首页> 外文期刊>Journal of Biotechnology >Natural endophytic association between Rhizobium etli and maize (Zea mays L.)
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Natural endophytic association between Rhizobium etli and maize (Zea mays L.)

机译：根瘤菌与玉米（Zea mays L.）之间的天然内生缔合

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Maize (Zea mays) and bean (Phaseolus vulgaris) have been traditionally grown in association for thousands of years in Mesoamerica. From surface sterilized maize roots, we have isolated over 60 Rhizobium strains that correspond to Rhizobium edi bv. phaseoli (the main symbiont of bean) on the basis of 16S rRNA gene restriction patterns, metabolic enzyme electropherotypes, organization of nif genes, and the ability to nodulate beans. The colonization capacity of some of the isolates was tested with an unimproved maize cultivar and with 30 maize land races. Increases in plant dry weight upon R. etli inoculation were recorded with some of the land races, and these increases may be related to plant growth promotion effects. Additionally, from within maize grown in monoculture we have also recovered R. etli isolates recognizable by their 16S rRNA gene types, which lack nif genes and are incapable of nodulating bean. These strains are presumed to correspond to the earlier described non-symbiotic R. etli obtained from bean rhizosphere.
机译：玉米（Zea mays）和豆类（菜豆（Phaseolus vulgaris））在中美洲传统上是联合栽培的，已有数千年的历史了。从表面灭菌的玉米根中，我们分离出了60多种对应于edi bv的根瘤菌菌株。菜豆（菜豆的主要共生体）基于16S rRNA基因的限制模式，代谢酶电特征，nif基因的组织以及结瘤的能力。使用未经改良的玉米品种和30个玉米陆地种测试了某些分离株的定殖能力。在某些陆生种族中，接种R. etli后记录到植物干重的增加，这些增加可能与促进植物生长的作用有关。此外，从单一培养的玉米中，我们还回收了可通过其16S rRNA基因类型识别的R. etli分离株，这些分离株缺乏nif基因且无法结瘤。推测这些菌株对应于较早描述的从豆根际获得的非共生R. etli。

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