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首页> 外文期刊>Journal of Biotechnology >Long-term preservation of rat pancreatic islets under physiologicalconditions
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Long-term preservation of rat pancreatic islets under physiologicalconditions

机译：生理条件下大鼠胰岛的长期保存

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摘要

In this study, we found that islet cells treated with polyphenol could be preserved for over 2 months under physiological conditions retaining their original function and maintaining their spherical shapes without any insulin secretion. When islets were treated at higher concentration than 250 mug ml(-1), these islets could retain their compact spherical shape over 65 days whereas non-treated islets were scattered ease to break within 2 weeks. The secretional capacity from treated islets in the initial stage is also lower than untreated islets. However, in the case of untreated islets, insulin release rapidly lowered with the progress in the culture time and secretion completely disappeared after 9 days. On the contrary, islets treated with polyphenol (250 mug ml(-1)) in RPMI culture medium showed significant enhancement of insulin secretion on 40th day. The secretional capacity of islets was greatly dependent on the treating concentration. Polyphenol treatment may be a useful method for preservation of mammalian islet cells. By changing the concentration of polyphenol, it is possible to control the preservation duration and insulin secretion of islets.
机译：在这项研究中，我们发现用多酚处理的胰岛细胞可以在生理条件下保存2个月以上，保持其原始功能并保持其球形，而无需任何胰岛素分泌。当以高于250毫升/ ml（-1）的浓度处理胰岛时，这些胰岛可以在65天之内保持其紧凑的球形形状，而未处理的胰岛则在2周内容易散开并破裂。初始阶段，处理过的胰岛的分泌能力也低于未处理过的胰岛。但是，对于未经处理的胰岛，随着培养时间的延长，胰岛素释放迅速降低，并且分泌在9天后完全消失。相反，在RPMI培养基中用多酚（250毫升（-1））处理的胰岛在第40天显示出胰岛素分泌的显着增强。胰岛的分泌能力在很大程度上取决于治疗浓度。多酚处理可能是保存哺乳动物胰岛细胞的有用方法。通过改变多酚的浓度，可以控制胰岛的保存时间和胰岛素分泌。

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