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首页> 外文期刊>Journal of Biotechnology >Expression of arabinogalactan proteins involved in Taxol production by immobilized Taxus cuspidata cells
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Expression of arabinogalactan proteins involved in Taxol production by immobilized Taxus cuspidata cells

机译：固定化红豆杉细胞表达涉及紫杉醇的阿拉伯半乳聚糖蛋白的表达

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摘要

The expression of arabinogalactan-proteins (AGPs), known as extracellular signal molecules, in immobilized T. cuspidata cells was investigated by immunofluorescence localization and Western blot analysis. It was found that the relative intensity of JIM13-reactive AGPs and Taxol production by T. cuspidata cells was increased 1.43-fold and 2.2-fold by immobilized cultures on day 25, respectively. Particularly, the expression levels of JIM13-reactive AGPs were much higher in the cells located in central and middle zones of the immobilized support matrices than these in the outer zone or in the suspension. Whether in immobilized T. cuspidata cells or in suspended T. cuspidata cells, the expression level of JIM13-reactive AGPs and Taxol production after two to three subcultures had no significant changes, but the immobilized cells always kept high-level expression of JIM13-reactive AGP and Taxol production during subcultures. Moreover, the enhancement of Taxol production was accompanied with a high-level expression of JIM13-reactive AGPs by T. cuspidata cells after treatment with 200 microM methyl jasmonate. Taken together, these results implicate that the AGPs in T. cuspidata cells may be taken as potential signal molecular involved in regulating the Taxol production by immobilized T. cuspidata cells.
机译：通过免疫荧光定位和Western印迹分析研究了阿拉伯半乳聚糖蛋白（AGP），称为细胞外信号分子，在固定的虎杖细胞中的表达。发现通过固定培养在第25天，虎杖T.cuspidata细胞的JIM13反应性AGP和紫杉醇产生的相对强度分别增加了1.43倍和2.2倍。特别地，在固定化支持基质的中央和中间区域中的细胞中，JIM13反应性AGP的表达水平比外部区域或悬浮液中的高得多。无论在固定的虎杖细胞中还是在悬浮的虎杖细胞中，经过两至三次传代培养，JIM13反应性AGP的表达水平和紫杉醇的产量均无显着变化，但固定化细胞始终保持高表达的JIM13反应性亚培养期间的AGP和紫杉醇生产。此外，在用200 microM茉莉酸甲酯处理后，由T. cuspidata细胞高表达JIM13反应性AGP伴随着紫杉醇的产生。两者合计，这些结果暗示，可将虎杖细胞中的AGP视为潜在的信号分子，参与通过固定的虎杖细胞调节紫杉醇的产生。

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