...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Biotechnology >Effects of acid concentration and solvent choice on enzymatic acrylation by Candida antarctica lipase B
【24h】

Effects of acid concentration and solvent choice on enzymatic acrylation by Candida antarctica lipase B

机译：酸浓度和溶剂选择对南极假丝酵母脂肪酶B酶促丙烯酸化的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lipase-mediated acrylation is an attractive alternative to more traditional chemical processes, since it provides specific catalysis under mild conditions. A detailed study of the effects of solvent choice and substrate concentrations on the acrylation of octanol by Candida antarctica lipase B (Novozym((R)) 435) is presented. Acrylic acid was found to have a pronounced inhibitory effect. Partial neutralisation of the acid substrate by addition of an organo-soluble base markedly altered the activity profile, indicating the inhibitory mechanism to be related to acid-base interactions. The concentration of acrylic acid to be employed was found to be important in the choice of an appropriate solvent. At low acrylic acid concentrations, the highest rates and conversions were obtained using hydrophobic solvents, whereas at higher acrylic acid concentrations more polar solvents were advantageous.
机译：脂肪酶介导的丙烯酸化是较传统的化学方法的一种有吸引力的替代方法，因为它在温和的条件下提供特定的催化作用。提出了关于溶剂选择和底物浓度对南极假丝酵母脂肪酶B（Novozym（435））辛醇丙烯酸化的影响的详细研究。发现丙烯酸具有明显的抑制作用。通过添加有机可溶性碱来部分中和酸底物会显着改变活性谱，表明抑制机制与酸碱相互作用有关。发现所用丙烯酸的浓度在选择合适的溶剂中很重要。在低丙烯酸浓度下，使用疏水性溶剂可获得最高的转化率和转化率，而在较高丙烯酸浓度下，更多极性溶剂是有利的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号