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首页> 外文期刊>Journal of Biotechnology >Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori
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Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori

机译：利用家蚕启动子在昆虫细胞系中构建基因表达系统

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摘要

The promoter regions of the Bombyx mori HSC70-4 and B. mori TCTP genes characterized previously were used for the construction of a series of constitutive gene expression systems active in cultured cells. The relative abilities of these promoters were evaluated by comparing those of a silkworm actin A3 (BmActin3) promoter, which is used widely as the first choice. A series of constitutive expression systems constructed were assayed for the transcription efficiency by connecting four reporter cDNAs, firefly luciferase, 3GFP, Ds-Red, and beta-galactosidase gene using the Gateway LR reaction. The insertion of an intron enhancer into the site between the TCTP promoter and gene increased the transcription of the BmTCTP promoter by 10-fold. The insertion of the IE-1 gene and HR3 enhancer to the all three promoters were found to increase the transcription up to 560 times.
机译：先前表征的家蚕HSC70-4和家蚕TCTP基因的启动子区域用于构建在培养细胞中具有活性的一系列组成型基因表达系统。通过比较被广泛用作首选的家蚕肌动蛋白A3（BmActin3）启动子的能力，评估了这些启动子的相对能力。通过使用Gateway LR反应连接四个报告基因cDNA，萤火虫荧光素酶，3GFP，Ds-Red和β-半乳糖苷酶基因，测定了一系列构建的组成型表达系统的转录效率。将内含子增强子插入TCTP启动子和基因之间的位点使BmTCTP启动子的转录增加了10倍。 IE-1基因和HR3增强子插入所有三个启动子被发现增加转录达560次。

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