...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Biotechnology >Additional virulence genes in conjunction with efficient selection scheme, and compatible culture regime enhance recovery of stable transgenic plants in cowpea via Agrobacterium tumefaciens-mediated transformation
【24h】

Additional virulence genes in conjunction with efficient selection scheme, and compatible culture regime enhance recovery of stable transgenic plants in cowpea via Agrobacterium tumefaciens-mediated transformation

机译：通过高效农杆菌介导的转化，其他毒力基因与有效的选择方案和兼容的培养机制相结合，可提高cow豆中稳定转基因植物的回收率

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A critical step in the development of robust Agrobacterium tumefaciens-mediated transformation system in recalcitrant grain legume, cowpea is the establishment of optimal conditions for efficient T-DNA delivery into target tissue and recovery of transgenic plants. A dramatic increase in efficiency of T-DNA delivery was achieved by constitutive expression of additional vir genes in resident pSB1 vector in Agrobacterium strain LBA4404. A geneticin based selection system permitted rapid and efficient identification of transgenic shoots without interfering with their regeneration, and eliminated the bulk of escapes. Supplementation of 0.5gammaM kinetin to medium containing 5.0gammaM benzyl aminopurine after 1 week of culture followed by 3 weeks of culture were found critical for optimal multiplication and elongation of transformed shoots from cotyledonary node explants. Combining these three developments, we recovered fertile transgenic plants at a frequency of 1.64%, significantly higher than previous reports. The presence, integration, expression and inheritance of transgenes were confirmed by molecular analysis. The protocol developed for cultivar Pusa Komal will facilitate the transfer of desirable traits into cowpea.
机译：在顽固的谷物豆科植物中，发展强大的根癌农杆菌介导的转化系统的关键步骤是cow豆的建立，该条件是将T-DNA有效递送至目标组织和回收转基因植物的最佳条件。通过在农杆菌菌株LBA4404中的驻留pSB1载体中额外表达vir基因组成性表达，可实现T-DNA传递效率的显着提高。基于遗传蛋白的选择系统可快速有效地鉴定转基因芽，而不会干扰其再生，并消除了大量逃逸。培养1周后再培养3周后，向含有5.0 gM M苄基氨基嘌呤的培养基中补充0.5 gammaM激动素对于从子叶节外植体转化的芽的最佳繁殖和伸长至关重要。结合这三个方面的发展，我们以1.64％的频率回收了可育的转基因植物，大大高于以前的报道。通过分子分析证实了转基因的存在，整合，表达和遗传。为品种Pusa Komal开发的协议将促进将所需性状转移到cow豆中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号