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首页> 外文期刊>Journal of Biotechnology >AFLP fingerprinting as a tool to study the genetic diversity of Rhizobium galegae isolated from Galega orientalis and Galega officinalis
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AFLP fingerprinting as a tool to study the genetic diversity of Rhizobium galegae isolated from Galega orientalis and Galega officinalis

机译：AFLP指纹图谱作为研究东方侧柏和加里加银耳分离的半球形根瘤菌遗传多样性的工具

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摘要

AFLP fingerprints of Rhizobium galegae strains that infect Galega orientalis and Galega officinalis obtained from different geographical sources, and of taxonomically diverse rhizobia representing the recognized species, were generated. Comparisons of the fingerprints from fluorescent labeled AFLP products using capillary electrophoresis on ABI prism 310, slab gel electrophoresis on ABI prism 377 genetic analyzers and silver staining were in good agreement. All methods delineated the G. orientalis strains from G. officinalis strains, the G. orientalis strains formed a tight cluster whereas the G. officinalis strains seem to show a greater level of genetic diversity. Comparison of fluorescent AFLP with other detection methods revealed that fluorescent labeling is more sensitive and practical, in addition, the deleterious effect of radioactivity associated with 32P-labeling, the delicate process of blotting polyacrylamide gels or the tedious procedure of silver staining can be avoided. The automated system facilitated a large number of runs at a time and the subsequent analysis of the data by generating exportable raw data. The congruency of the experiments was analyzed using the Bionumerics software.
机译：产生了感染从不同地理来源获得的侧柏和厚朴的根瘤菌菌株的AFLP指纹图谱，以及代表公认物种的分类学上不同的根瘤菌的AFLP指纹图谱。使用ABI棱镜310上的毛细管电泳，ABI棱镜377遗传分析仪上的平板凝胶电泳和银染色比较荧光标记的AFLP产品的指纹。所有的方法都从厚皮草菌株中划定了侧柏菌株，侧柏菌株形成紧密的簇，而厚皮菌株似乎显示出更高水平的遗传多样性。荧光AFLP与其他检测方法的比较表明，荧光标记更为灵敏和实用，此外，可以避免与32P标记有关的放射性有害作用，聚丙烯酰胺凝胶印迹的精细过程或银染的繁琐程序。自动化系统通过生成可导出的原始数据，促进了一次大量运行以及随后的数据分析。使用生物数字软件分析实验的一致性。

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