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首页> 外文期刊>Journal of Biotechnology >Quantification of the contribution of biological nitrogen fixation to tropical green manure crops and the residual benefit to a subsequent maize crop using N-15-isotope techniques
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Quantification of the contribution of biological nitrogen fixation to tropical green manure crops and the residual benefit to a subsequent maize crop using N-15-isotope techniques

机译：使用N-15同位素技术量化固氮对热带绿肥作物的贡献以及对随后的玉米作物的残留效益

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摘要

In this study the contribution of biological N-2 fixation (BNF) to leguminous green manures was quantified in the field at different sites with different N-15 methodologies. In the first experiment, conducted on a Terra Roxa soil in Cuba, the BNF contribution to three legumes (Crotalaria juncea, Mucuna aterrima and Canavalia ensiformis) was quantified by applying N-15-labelled ammonium sulphate to the soil. The second experiment was planted in a very low fertility sandy soil near Rio de Janeiro, and the N-15 natural abundance technique was applied to quantify BNF in C. juncea, M. niveum and soybean. In both studies the advantages of using several non-N-2-fixing reference plants was apparent and despite the much greater accumulation of the C. juncea in the experiment performed on the fertile soil of Cuba, the above ground contributions of BNF at both sites were similar (40-80 kg N ha(-1)) and greater than for the other legumes. In a further experiment the possible contribution of root-derived N to the soil/plant system of two of the legumes was quantified using a N-15-leaf-labelling technique performed in pots. The results of this study suggested that total below-ground N could constitute as much as 39 to 49% of the total N accumulated by the legume crops.
机译：在这项研究中，使用不同的N-15方法在不同地点的田间量化了生物N-2固定（BNF）对豆科绿肥的贡献。在古巴的Terra Roxa土壤上进行的第一个实验中，通过将N-15标记的硫酸铵施用到土壤中来定量BNF对三种豆类（Crotalaria juncea，Mucuna aterrima和Canavalia ensiformis）的贡献。第二个实验种植在里约热内卢附近肥沃的低土壤土壤中，N-15天然丰度技术用于定量分析芥蓝，念珠菌和大豆中的BNF。在这两项研究中，使用几种非N-2固定参照植物的优势显而易见，尽管在古巴的肥沃土壤上进行的芥菜梭菌积累量更大，但BNF在两个地点的地上贡献相似（40-80 kg N ha（-1）），大于其他豆类。在进一步的实验中，使用在盆中进行的N-15叶标记技术定量了根系氮对两种豆科植物的土壤/植物系统的可能贡献。这项研究的结果表明，地下总氮量可能占豆类作物累积总氮量的39％至49％。

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