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首页> 外文期刊>Journal of Biotechnology >Optimization of astaxanthin production by Phaffia rhodozyma throughfactorial design and response surface methodology
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Optimization of astaxanthin production by Phaffia rhodozyma throughfactorial design and response surface methodology

机译：通过因子设计和响应面法优化红发夫夫酵母生产虾青素的方法

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Sequential methodology based on the application of three types of experimental designs was used to optimize the astaxanthin production of the mutant strain 25-2 of Phaffia rhodozyma in shake flask cultures. The first design employed was a factorial design 2(5), where the factors studied were: pH, temperature, percent of inoculum, carbon and nitrogen concentrations, each one at two levels. This design was performed in two medium types: rich YM medium and minimal medium, based on date juice (Yucca medium). With this first design the most important factors were determined (carbon concentration and temperature) that were used in the second experimental strategy: the method of steepest ascent was applied in order to rapidly approach the optimum. Finally, a second-order response surface design was applied using temperature and carbon concentration as factors. The optimal conditions stimulating the highest astaxanthin production were: 19.7 degreesC temperature; 11.25 g l(-1) carbon concentration; 6.0 pH; 5% inoculum and 0.5 g l(-1) nitrogen concentration. Under these conditions the astaxanthin production was 8100 mug l(-1), 92% higher than the production under the initial conditions.
机译：使用基于三种类型的实验设计的顺序方法学来优化摇瓶培养中红发夫夫酵母突变株25-2的虾青素生产。所采用的第一个设计是析因设计2（5），其中研究的因素是：pH，温度，接种物百分比，碳和氮浓度，每一个处于两个水平。基于枣汁（Yucca培养基），在两种培养基类型中进行了这种设计：富YM培养基和极少培养基。通过第一个设计，确定了在第二个实验策略中使用的最重要的因素（碳浓度和温度）：采用最陡峭上升的方法以快速达到最佳状态。最后，以温度和碳浓度为因子，进行了二次响应表面设计。刺激虾青素产量最高的最佳条件是：温度19.7摄氏度; 11.25 g l（-1）的碳浓度; 6.0 pH;接种量为5％，氮浓度为0.5 g l（-1）。在这些条件下，虾青素的产量为8100马克杯l（-1），比初始条件下的产量高92％。

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